scholarly journals A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos

Zebrafish ◽  
2020 ◽  
Vol 17 (4) ◽  
pp. 268-270
Author(s):  
Julie Somkhit ◽  
Roxane Loyant ◽  
Alexandre Brenet ◽  
Rahma Hassan-Abdi ◽  
Constantin Yanicostas ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Zebrafish Embryos ◽  
Measure Oxygen Consumption

Oxygen requirements of the dyskinetic myocardial segment

1989 ◽  
Vol 257 (4) ◽  
pp. H1184-H1191 ◽  
Author(s):  
P. A. Gayheart ◽  
J. Vinten-Johansen ◽  
W. E. Johnston ◽  
T. O. Hester ◽  
A. R. Cordell
Keyword(s):  
Blood Flow ◽  
Oxygen Consumption ◽  
Coronary Arteries ◽  
Dose Response ◽  
Contractile Function ◽  
Myocardial Segment ◽  
Response Curves ◽  
Intracoronary Infusion ◽  
Oxygen Requirements ◽  
Measure Oxygen Consumption

Oxygen requirements of a noncontracting myocardial segment subjected to passive systolic stretch (dyskinesis) have not been well described. The purpose of this study was to measure oxygen consumption (MVO2) of a myocardial segment made dyskinetic by intracoronary infusion of lidocaine. In 12 anesthetized open-chest dogs, segmental shortening was measured sonomicrometrically in regions perfused by the left anterior descending (LAD) and circumflex (Cfx) coronary arteries. MVO2 was measured by arterial-venous oxygen content differences and transmural blood flow. Dose-response curves to intracoronary lidocaine showed that complete dyskinesis was achieved by a 0.25-mg/ml dose of lidocaine, whereas the Cfx region maintained a constant level of segmental shortening. MVO2 of the LAD segment was similar to that of the Cfx segment under control conditions. With lidocaine-induced dyskinesis, MVO2 in the arrested segment was reduced by 33% (P less than 0.05), despite the loss of contractile function. When bulging was prevented by ventricular unloading, MVO2 in the arrested segment decreased to 2.65 ml O2.min-1.100 g-1 (i.e., basal oxygen requirements). In conclusion, MVO2 in a pharmacologically arrested myocardial segment undergoing systolic bulging is paradoxically high relative to both basal requirements and MVO2 in the normally contracting segment.

Physical and Chemical Characteristics of Cultus Lake, British Columbia.

1937 ◽  
Vol 3 (4) ◽  
pp. 363-402 ◽  
Author(s):  
William E. Ricker
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Content ◽  
Heat Budget ◽  
Clear Lake ◽  
Equable Climate ◽  
Low Degree ◽  
Seasonal Circulation ◽  
Measure Oxygen Consumption ◽  
Physical And Chemical

Cultus lake is a rather small, fairly deep, clear lake of the western Cascade mountains, and is the home of one of the populations of Fraser river sockeye. The equable climate of the region is reflected in the low annual heat budget of the lake and its normal lack of an ice cover in winter, with continued complete or partial circulation of its water throughout that season. Judged by the low degree of hypolimnial oxygen consumption in summer, the lake is to be classed as distinctly oligotrophic. The oxygen content at all depths and seasons is sufficient for the respiration of salmonoid fishes. The water is moderately hard and slightly alkaline. Quantities of dissolved nitrates and phosphates in the water appear to be of the order characteristic of oligotrophic lakes. Silicates are present much in excess of the requirements of the lake's diatom populations. The number of adult sockeye returning to the lake in normal years is insufficient to add to its supply of nutritive salts in significant amount. Temperature observations and oxygen determinations, taken at frequent intervals for several years, have contributed to the knowledge of certain questions of general limnological interest. Such include (1) the manner in which the hypolimnion of a lake is warmed in summer; (2) the classification of the seasonal circulation periods in temperate lakes; and (3) the oxygen content of a lake at the close of vernal circulation, and its use as a standard from which to measure oxygen consumption throughout the summer.

scholarly journals Growth of Spiny Lobster Panulirus homarus Fed with Moist Diet

2018 ◽  
Vol 3 (2) ◽  
pp. 95
Author(s):  
Asep Ridwanudin ◽  
Varian Fahmi ◽  
Idham Sumarto Pratama
Keyword(s):  
Oxygen Consumption ◽  
Dissolved Oxygen ◽  
Spiny Lobster ◽  
Growth And Survival ◽  
Oxygen Levels ◽  
Hypoxic Conditions ◽  
National University ◽  
Oxygen Conditions ◽  
Measure Oxygen Consumption

Oxygen is a vital parameter in aquaculture activities. The decrease of dissolved oxygen levels in aquaculture media should be highly observed, since very low dissolved oxygen conditions (hypoxia) could negatively affect to the growth and survival of fish. Therefore, research on the condition of hypoxia is very important to be studied. This study was conducted in January-February 2013 at the Laboratorium of Fish Reproductive Physiology, Pukyong National University, South Korea to measure oxygen consumption in tilapia Oreochromis niloticus (total length: 14.2 ± 1.4 cm, weight: 31.3 ± 2.0 g) under normal conditions (normoxia) and hypoxia. Measurement was conducted using respirometer (dimension: 20 × 17,5 × 10 cm; volume: 3,5 L) every 10 minutes during four hours of observation. Experiment was conducted with three replications. The results showed that oxygen consumption of tilapia fingerlings in hypoxia conditions (12.09 ± 3.20 mg O2/kg/h) was lower than normoxia (35.67 ± 4.19 mg O2/kg/h) (P<0.01). Continuous hypoxic conditions could negatively affect fish movements, which could ultimately lead to mortality when dissolved oxygen levels are very low. Meanwhile, the results on determination of critical oxygen levels for tilapia showed a dissolved oxygen range of 1.9 ± 0.5 mg/L

scholarly journals Indirect Calorimetry Overestimates Oxygen Consumption in Young Children: Caution is Advised Using Direct Fick Method as a Reference Method in Cardiac Output Comparison Studies

2019 ◽  
Vol 41 (1) ◽  
pp. 149-154
Author(s):  
Theodor S. Sigurdsson ◽  
Lars Lindberg
Keyword(s):  
Cardiac Output ◽  
Oxygen Consumption ◽  
Young Children ◽  
Indirect Calorimetry ◽  
Method Comparison ◽  
Reference Method ◽  
Percentage Error ◽  
Measure Oxygen Consumption ◽  
A Minor ◽  
Method Comparison Study

AbstractDirect Fick method is considered a standard reference method for estimation of cardiac output. It relies on indirect calorimetry to measure oxygen consumption. This is important as only a minor measurement error in oxygen consumption can result in false estimation of cardiac output. A number of studies have shown that indirect calorimetry overestimates oxygen consumption in adults. The aim of this prospective single center observational method comparison study was to compare the determination of oxygen consumption by indirect calorimetry and reverse Fick method in pediatric patients. Forty-two children mean age 352 days (range 30 to 1303 days) and mean weight 7.1 kg (range 2.7–13.6 kg) undergoing corrective cardiac surgery were included in the study. The mean (standard deviation) oxygen consumption by reverse Fick method was 43.5 (16.2) ml/min and by indirect calorimetry 49.9 (18.8) ml/min (p < 0.001). Indirect calorimetry overestimated the reverse Fick oxygen consumption by 14.7%. Bias between methods was 6.5 (11.3) ml/min, limits of agreement (LOA) − 15.7 and 28.7 ml/min and percentage error of 47.7%. A significant bias and large percentage error indicates that the methods are not interchangeable. Indirect calorimetry and the direct Fick method should be used with caution as a reference method in cardiac output comparison studies in young children.

Sign in / Sign up

Export Citation Format

Share Document