scholarly journals The Role of the Kinesin Motor KipA in Microtubule Organization and Polarized Growth ofAspergillus nidulans

2005 ◽  
Vol 16 (2) ◽  
pp. 497-506 ◽  
Author(s):  
Sven Konzack ◽  
Patricia E. Rischitor ◽  
Cathrin Enke ◽  
Reinhard Fischer
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Lateral Movement ◽  
Polarized Growth ◽  
Microtubule Organization ◽  
Kinesin Motor ◽  
Ofaspergillus Nidulans ◽  
Green Fluorescent ◽  
Intrinsic Motor

Polarized growth in filamentous fungi requires the integrity of the microtubule (MT) cytoskeleton. We found that growing MTs in Aspergillus nidulans merge at the center of fast growing tips and discovered that a kinesin motor protein, KipA, related to Tea2p of Schizosaccharomyces pombe, is required for this process. In a ΔkipA strain, MT plus ends reach the tip but show continuous lateral movement. Hyphae lose directionality and grow in curves, apparently due to mislocalization of the vesicle supply center (Spitzenkörper) in the apex. Green fluorescent protein (GFP)-KipA accumulates at MT plus ends, whereas a KipA rigor mutant protein, GFP-KipAG223E, coated MTs evenly. These findings suggest that KipA requires its intrinsic motor activity to reach the MT plus end. Using KipA as an MT plus-end marker, we found bidirectional organization of MTs and determined the locations of microtubule organizing centers at nuclei, in the cytoplasm, and at septa.

Defining the Role of Arginine 96 in Green Fluorescent Protein Fluorophore Biosynthesis†,‡

Biochemistry ◽  
2005 ◽  
Vol 44 (49) ◽  
pp. 16211-16220 ◽  
Author(s):  
Timothy I. Wood ◽  
David P. Barondeau ◽  
Chiharu Hitomi ◽  
Carey J. Kassmann ◽  
John A. Tainer ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals ROLE OF GREEN-FLUORESCENT-PROTEIN-EXPRESSING T CELLS IN EXPERIMENTAL AUTOIMMUNE MYOCARDITIS

2011 ◽  
Vol 57 (14) ◽  
pp. E228
Author(s):  
Tomoyoshi Yanagisawa ◽  
Takayuki Inomata ◽  
Ichiro Watanabe ◽  
Emi Maekawa ◽  
Tomohiro Mizutani ◽  
...  
Keyword(s):  
T Cells ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Autoimmune Myocarditis ◽  
Experimental Autoimmune Myocarditis ◽  
Green Fluorescent ◽  
Experimental Autoimmune

scholarly journals Experimental Model of Human Body Louse Infection Using Green Fluorescent Protein-Expressing Bartonella quintana

2001 ◽  
Vol 69 (3) ◽  
pp. 1876-1879 ◽  
Author(s):  
Pierre-Edouard Fournier ◽  
Michael F. Minnick ◽  
Hubert Lepidi ◽  
Eric Salvo ◽  
Didier Raoult
Keyword(s):  
Green Fluorescent Protein ◽  
Human Body ◽  
Fluorescent Protein ◽  
Rabbit Model ◽  
Bartonella Quintana ◽  
Laboratory Colony ◽  
Green Fluorescent ◽  
Human Body Louse ◽  
Body Lice

ABSTRACT A laboratory colony of human body lice was experimentally infected by feeding on rabbits made artificially bacteremic with a green fluorescent protein-expressing Bartonella quintana. B. quintana was detected in the gut and feces until death but not in the eggs. The life span of the lice was not modified. The rabbit model should provide valuable clues to the role of lice in the transmission of B. quintana.

Deletion of bem46 retards spore germination and may be related to the polar growth of Aspergillus fumigatus

2019 ◽  
Vol 58 (5) ◽  
pp. 690-697
Author(s):  
Yan Ma ◽  
Ying Ji ◽  
Jing Yang ◽  
Wen Li ◽  
Jiajuan Li ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Laser Scanning ◽  
Fluorescent Protein ◽  
Morphological Changes ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Polarized Growth ◽  
Bud Emergence ◽  
Green Fluorescent

Abstract Bud emergence 46 (BEM46), a member of the α/β hydrolase superfamily, has been reported to be essential for polarized growth in Neurospora crassa. However, the role of BEM46 in aspergillus fumigatus (A. fumigatus) remains unclear. In this study, we constructed an A. fumigatus strain expressing BEM46 fused with enhanced green fluorescent protein, and a Δbem46 mutant, to explore the localization and the role of growth of BEM46 in A. fumigatus, respectively. Confocal laser scanning microscopy revealed that BEM46 was dominantly expressed in the sites where hyphae germinated from conidia in A. fumigatus. When compared with the control strain, the Δbem46 mutant exhibited insignificant morphological changes but delayed germination. No significant changes were found regarding the radial growth of both strains in response to various antifungal agents. These results suggest that BEM46 plays an essential role in timely germination in A. fumigatus. From the observation of fluorescence localization, we infer that that BEM46 might be involved in polarized growth in A. fumigatus.

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