Advanced glycation end products of bovine serum albumin affect the cell growth of human umbilical vein endothelial cells via modulation of MEG3/miR-93/p21 pathway

The aim of this study was to investigate the effects of advanced glycation end products (AGEs) on the expression and activity of matrix metalloproteinases-2 (MMP-2) in human umbilical vein endothelial cells (HUVECs). Cultured HUVECs were incubated with various concentrations of AGEs-modified albumin or unmodified albumin for different time periods. Protein and gene expression of MMP-2 and the receptor for AGEs (RAGE) were measured by Western blot and reverse transcription-polymerase chain reaction, respectively. The activity of MMP-2 in the conditioned medium was measured by gelatin zymography. The AGE-modified albumin inhibited MMP-2 but increased RAGE protein and gene expression in HUVECs in a concentration- and time-dependent manner. An inhibition of MMP-2 activity was also detected in the conditioned medium of HUVECs incubated with AGEs-modified albumin. In conclusion, AGEs inhibited the expression and activity of MMP-2 in HUVECs; this may be mediated through upregulation of RAGE.

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Thiamine corrects delayed replication and decreases production of lactate and advanced glycation end-products in bovine retinal and human umbilical vein endothelial cells cultured under high glucose conditions

Diabetologia ◽

10.1007/s001250050568 ◽

1996 ◽

Vol 39 (11) ◽

pp. 1263-1268 ◽

Cited By ~ 59

Author(s):

M. La Selva ◽

E. Beltramo ◽

F. Pagnozzi ◽

E. Bena ◽

P. A. Molinatti ◽

...

Keyword(s):

Endothelial Cells ◽

Advanced Glycation End Products ◽

High Glucose ◽

Umbilical Vein ◽

Advanced Glycation ◽

Human Umbilical Vein ◽

Glycation End Products ◽

End Products ◽

Umbilical Vein Endothelial Cells ◽

Cells Cultured

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Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells

Journal of Diabetes Research ◽

10.1155/2013/105780 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Jing Liu ◽

Liping Yan ◽

Ruilan Niu ◽

Limin Tian ◽

Qi Zhang ◽

...

Keyword(s):

Nitric Oxide ◽

Endothelial Cells ◽

Bovine Serum Albumin ◽

Advanced Glycation End Products ◽

Bovine Serum ◽

Advanced Glycation ◽

Protection Effect ◽

Glycation End Products ◽

End Products ◽

Oxide Synthase

Endomorphins (EMs) have a very important bridge-function in cardiovascular, endocrinological, and neurological systems. This study is to investigate the effects of EMs on the synthesis and secretion of vasoactive substances induced by advanced glycation end products in primary cultured human umbilical vein endothelial cells (HUVECs). Firstly, HUVECs were stimulated with AGEs-bovine serum albumin (AGEs-BSA), bovine serum albumin (BSA), or both AGEs-BSA and EMs together, respectively. Then, HUVEC survival rate was calculated by MTT assay, the levels of NO, endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) were detected by colorimetric analysis, and the contents of endothelin-1 (ET-1) were detected by ELISA. The mRNA levels of eNOS and ET-1 were measured by RT-PCR. The expression of p38 mitogen-activated protein kinase (p38 MAPK) was detected by immunofluorescence assay. The results showed that the mRNA expression and secretion of eNOS were significantly enhanced after incubation with EMs compared to those with AGEs-BSA, while the secretion of NO and iNOS, mRNA expression, and secretion of ET-1 had opposite changes. The fluorescence intensity of p38MAPK in nuclear was decreased after pretreatment with EMs compared to incubation with AGEs-BSA.Conclusion. The present study suggests that EMs have certain protection effect on AGEs-BSA-induced injury in HUVEC.

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