A Slide Latex-Particle Agglutination Test for Trichinosis

1962 ◽  
Vol 37 (2_ts) ◽  
pp. 227-231 ◽  
Author(s):  
Thelma F. Muraschi ◽  
Norman Bloomfield ◽  
Robert B. Newman
PEDIATRICS ◽  
1982 ◽  
Vol 69 (4) ◽  
pp. 466-471
Author(s):  
Robert S. Daum ◽  
George R. Siber ◽  
Jill S. Kamon ◽  
Rebecca R. Russell

The effectiveness of a commercially available latex particle agglutination test (Bactogen) in the diagnosis of invasive Haemophilus influenzae type b infection was evaluated. Bactogen correctly diagnosed all 27 patients with bacteriologically proven H influenzae type b infection (sensitivity 100%). Two of 39 patients with proven, non-H influenzae type b infections had false-postive tests (specificity 95%). One of 103 sera and 0 of 55 urine specimens from hospitalized adults contained detectable H influenzae type b antigen. Bactogen is a sensitive, specific, commercially available test for rapid diagnosis of H influenzae type b infection.


2016 ◽  
Vol 1 (1) ◽  
pp. 10-12 ◽  
Author(s):  
Nurjahan Begum ◽  
Iftikhar Ahmed ◽  
Md. Abdus Salam ◽  
Shahanara Begum ◽  
KM Faisal Alam

A total  of 45 clinically suspected  cases of meningitis  of all  ages,  admitted at Rajshahi Medical  College  Hospital(RMCH),  were  studied to  find  out the etiological  agents of meningitis. Among  the cases,  32 (7l.1oh)  were diagnosed  asaseptic  meningitis  and  L3 (ZS.Sgoh)  as  bacterial meningitis by the  conventional  CSF  study. But  out of total  45 cases,lg  (40oh) were diagnosed as bacterial  meningitis  by  Latex  particle  agglutination test (LPAT).  The  LPAT  is  widelyused in  clinical  microbiology laboratories  and can  be considered  as an important  supplement  to the  culture  andGram stain of CSF specimens. Rapid  antigen  detection  tests may  provide positive results  when  culture  and  Gramstain results  are  negative for  meningitis patients  who have  received antimicrobial  therapy.  So, in our country, where empirical  broad-spectrum antibiotic is  randomly  used, LPAT may  be considered  as an adjunct  to establish  thediagnosis  of  bacterial  meningitis.Bangladesh  J Med Microbiol  2007;01(01):10-12


1968 ◽  
Vol 49 (1) ◽  
pp. 96-102 ◽  
Author(s):  
Milton Huppert ◽  
E. Taylor Peterson ◽  
S. H. Sun ◽  
A. Chitjian Patricia ◽  
J. Derrevere Westlyn

2016 ◽  
Vol 1 (1) ◽  
pp. 17-20
Author(s):  
Shahin Ara Begum ◽  
Aliya Rashid ◽  
Md. Abdur Rhman ◽  
Akhtaruzzman Chowdhury ◽  
Sufi HZ Rahman ◽  
...  

Streptococcus pneumoniae and Haemophilus influenzae type b are the main causes of community-acquired bacterial pneumonia in developing countries including Bangladesh. This study was carried out to find a rapid diagnostic method for early detection of bacterial pneumonia. Latex Particle Agglutination Test (LPAT) was done in urine for quick detection of capsular antigens of S. pneumoniae and H. influenzae type b. Three hundred children were enrolled based on clinical criteria as defined by the WHO. Among them, 70 pneumonic children, who had total white blood cell counts around  20000/ mm3, Neutrophils ? 75% and consolidation on chest X-rays, were selected for LPAT in urine. Some 20 children of the same age group, who had chest diseases other than pneumonia, were selected as sick control group. Among 70 pneumonic cases, 36 (51.43%) were positive by LPAT in urine. Out of 36 LPAT-positive cases, 19 (52.7%) were positive for S. pneumoniae and 17 (47.3%) were positive for H. influenzae type b by specific anti-sera. The LPAT is thus appears as a quick and reliable test for the diagnosis of bacterial pneumonia cases caused by S. pneumoniae and H. influenzae type b.Bangladesh J Med Microbiol 2007; 01 (01):17-20 


1977 ◽  
Vol 37 (02) ◽  
pp. 183-191 ◽  
Author(s):  
Victor J. Marder ◽  
Genuina O. Cruz ◽  
Bruce R. Schumer

SummaryA new latex particle agglutination test (FIBROTEX®) has been compared with the Thrombo-Wellcotest® and the tanned red cell hemagglutination inhibition immunoassay (TRCHII) for sensitivity to purified fibrinogen and plasmic degradation products and for reactivity with 31 normal sera and 170 sera from patients with thrombotic disorders. The latex particles in the FIBROTEX® are coated with antibodies against fibrinogen and fragment E (anti-FE), in contrast with the anti-fragment D and anti-fragment E antibodies which coat the latex particles of the Thrombo-Wellcotest® (anti-DE). The relative sensitivities of the TRCHII and the anti-FE and anti-DE latex tests were, respectively, 0.5, 2.5 and 7.5 μg/ml for fibrinogen; 0.5, 0.5 and 4.0 μg/ml for fragment X; and 1.25, > 30 and 8.0 μg/ml for fragment D. Compared with anti-DE, the anti-FE particles demonstrated fewer false-positive results with sera and urines of normals and higher titers, fewer false negatives and better overall correlation with the TRCHII results (r = 0.89 vs. 0.75) on pathologic sera. The FIBROTEX® is simple and rapid (5 minutes) and provides a more sensitive and accurate determination of serum fibrin degradation products than the Thrombo-Wellcotest®.


2007 ◽  
Vol 0 (0) ◽  
Author(s):  
Maria Jussara F. Fontes ◽  
Flávia B. Bottrel ◽  
Maria Tereza M. Fonseca ◽  
Laura B. Lasmar ◽  
Rosângela Diamante ◽  
...  

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