porcine origin
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2021 ◽  
Vol 19 (suplemento) ◽  
Author(s):  
M J Ruiz

Lactobacillus plantarum has a great capacity to adapt to environmental and have antibacterial capacity against different pathogen. This is a potential alternative for the control of pathogen in food coming slaughter animal. The objective of this work was to determine the antibacterial effect of L. plantarum strains against C. coli strains. L. plantarum LP1, LP3, LP5 and LP7 of porcine origin and a reference strain of Campylobacter coli NCTC 11366 were used. C. coli and L. plantarum was reactivated. Lyophilized ELC and ELCn were obtained. C. coli was distributed on the surface of mCCDA by swab and the ELC and the ELCn were inoculated in wells previously made on the agar. After 48 h, the diameter of the zone of inhibition was measured. The inhibition halos produced were 23.2 mm. The ELCn showed less antagonistic activity. The LP5 ELC generated higher inhibition halos and ELCn showed no differences between the four strains studied. L. plantarum strains, isolated from the pig production chain, could potentially be applied to control C. coli. This data is added to the scarce scientific evidence of the inhibitory effect of L. plantarum before this zoonotic pathogen of importance in public health.


Author(s):  
Erick Kipkirui ◽  
Margaret Koech ◽  
Abigael Ombogo ◽  
Ronald Kirera ◽  
Janet Ndonye ◽  
...  

Abstract Background Enterotoxigenic Escherichia coli (ETEC) is one of the leading causes of infectious diarrhea in children. There are no licensed vaccines against ETEC. This study aimed at characterizing Escherichia coli for ETEC enterotoxins and colonization factors from children < 5 years with acute diarrhea and had not taken antibiotics prior to seeking medical attention at the hospital. Methods A total of 225 randomly selected archived E. coli strains originally isolated from 225 children with acute diarrhea were cultured. DNA was extracted and screened by multiplex polymerase chain reaction (PCR) for three ETEC toxins. All positives were then screened for 11 colonization factors by PCR. Results Out of 225 E. coli strains tested, 23 (10.2%) were ETEC. Heat-stable toxin (ST) gene was detected in 16 (69.6%). ETEC isolates with heat-stable toxin of human origin (STh) and heat-stable toxin of porcine origin (STp) distributed as 11 (68.8%) and 5 (31.2%) respectively. Heat-labile toxin gene (LT) was detected in 5 (21.7%) of the ETEC isolates. Both ST and LT toxin genes were detected in 2 (8.7%) of the ETEC isolates. CF genes were detected in 14 (60.9%) ETEC strains with a majority having CS6 6 (42.9%) gene followed by a combination of CFA/I + CS21 gene detected in 3 (21.4%). CS14, CS3, CS7 and a combination of CS5 + CS6, CS2 + CS3 genes were detected equally in 1 (7.1%) ETEC isolate each. CFA/I, CS4, CS5, CS2, CS17/19, CS1/PCFO71 and CS21 genes tested were not detected. We did not detect CF genes in 9 (39.1%) ETEC isolates. More CFs were associated with ETEC strains with ST genes. Conclusion ETEC strains with ST genes were the most common and had the most associated CFs. A majority of ETEC strains had CS6 gene. In 9 (39.1%) of the evaluated ETEC isolates, we did not detect an identifiable CF.


Author(s):  
Simona Fioriti ◽  
Sonia Nina Coccitto ◽  
Gianluca Morroni ◽  
Serena Simoni ◽  
Marzia Cinthi ◽  
...  
Keyword(s):  

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 272
Author(s):  
Javier Aragoneses ◽  
Ana Suárez ◽  
Cinthia Rodríguez ◽  
Juan Manuel Aragoneses

This research aims to evaluate the clinical and histological parametric differences concerning keratinized tissue that result from two regeneration techniques, the subepithelial autologous connective tissue graft (ACTG) and the acellular dermal matrix (MD) of porcine origin, performed on surgical beds on edentulous spaces in an animal model. The parameters of the MD and ACTG groups were compared with samples of the control group (CG) after 15, 45, and 90 days. Nine female white pigs (Sus scrofa domestica) were used, and each animal provided 20 study areas (12 MD and 8 ACTG). At 15 days, the keratin layer thickness in the MD group was greater than those of the ACTG (25.27 vs. 19.95 μm) and the CG (21.2 μm). After 45 days, the MD and ACTG thickness values decreased but were higher than the CG. At 90 days, MD (19.46 μm) obtained a value close to that of CG, and the ACTG decreased to CG (15.53 μm, p < 0.001). The use of an MD may be a viable alternative to the ACTG because of its ability to provide increased keratinized tissue in comparison to the ACTG.


2021 ◽  
Vol 9 (1) ◽  
pp. 186-198
Author(s):  
Rita Sobreiro-Almeida ◽  
Maria Elena Melica ◽  
Laura Lasagni ◽  
Hugo Osório ◽  
Paola Romagnani ◽  
...  

Decellularized matrices are attractive substrates, being able to retain growth factors and proteins present in the native tissue.


2020 ◽  
Vol 8 (6) ◽  
pp. 896 ◽  
Author(s):  
Tiziana Zingali ◽  
Toni A. Chapman ◽  
John Webster ◽  
Piklu Roy Chowdhury ◽  
Steven P. Djordjevic

Antibiotic resistance genes (ARGs) including those from the blaCTX-M family and mcr-1 that encode resistance to extended spectrum β–lactams and colistin, respectively, have been linked with IncHI2 plasmids isolated from swine production facilities globally but not in IncHI2 plasmids from Australia. Here we describe the first complete sequence of a multiple drug resistance Australian IncHI2-ST4 plasmid, pTZ41_1P, from a commensal E. coli from a healthy piglet. pTZ41_1P carries genes conferring resistance to heavy-metals (copper, silver, tellurium and arsenic), β-lactams, aminoglycosides and sulphonamides. The ARGs reside within a complex resistance locus (CRL) that shows considerable sequence identity to a CRL in pSDE_SvHI2, an IncHI2:ST3 plasmid from an enterotoxigenic E. coli with serotype O157:H19 of porcine origin that caused substantial losses to swine production operations in Australia in 2007. pTZ41_1P is closely related to IncHI2 plasmids found in E. coli and Salmonella enterica from porcine, avian and human sources in Europe and China but it does not carry genes encoding resistance to clinically-important antibiotics. We identified regions of IncHI2 plasmids that contribute to the genetic plasticity of this group of plasmids and highlight how they may readily acquire new resistance gene cargo. Genomic surveillance should be improved to monitor IncHI2 plasmids.


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