Electronic Counting of Spinal Fluid Cells

Today, in image analysis the broadest possible rationalization and economization have become desirable. Basically, there are two approaches for image analysis: The image analysis through the so-called scanning methods which are usually performed without the human eye and the systems of optical semiautomatic analysis completely relying on the human eye.The new MOP AM 01 opto-manual system (fig.) represents one of the very promising approaches in this field. The instrument consists of an electronic counting and storing unit, which incorporates a microprocessor and a keyboard for choice of measuring parameters, well designed for easy use.Using the MOP AM 01 there are three possibilities of image analysis:the manual point counting,the opto-manual point counting andthe measurement of absolute areas and/or length (size distribution analysis included).To determine a point density for the calculation of the corresponding volume density the intercepts lying within the structure are scanned with the light pen.

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A New Technique for the Light and Electron Microscopic Study of Individual Cerebro-Spinal Fluid Cells in a Mona Plane Layer

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100091792 ◽

1976 ◽

Vol 34 ◽

pp. 362-363

Author(s):

L.A. Dell

Keyword(s):

Microscopic Study ◽

Electron Microscopic Study ◽

Plane Layer ◽

Ultrastructural Level ◽

Spinal Fluid ◽

Electron Microscopic Level ◽

Electron Microscopic ◽

Easy Method ◽

Cell Pellet ◽

A New Technique

A new method has been developed which readily offers the microscopist a possibility for both light and electron microscopic study of selected cells from the cerebrospinal fluid. Previous attempts to examine these cells in the spinal fluid at the ultrastructural level were based on modifications of cell pellet techniques developed for peripheral blood. These earlier methods were limited in application by the number of cells in spinal fluid required to obtain a sufficient size pellet and by the lack of an easy method of cellular identification between the light and electron microscopic level. The newly developed method routinely employs microscope slides coated with Siliclad and tungsten oxide for duplicate cytocentrifuge preparations of diagnostic spinal fluid specimens. Work done by Kushida and Suzuki provided a basis for our use of the metal oxide.

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