A New Technique for the Light and Electron Microscopic Study of Individual Cerebro-Spinal Fluid Cells in a Mona Plane Layer

Plane Layer ◽

Ultrastructural Level ◽

Spinal Fluid ◽

Electron Microscopic ◽

Easy Method ◽

Cell Pellet ◽

A New Technique

A new method has been developed which readily offers the microscopist a possibility for both light and electron microscopic study of selected cells from the cerebrospinal fluid. Previous attempts to examine these cells in the spinal fluid at the ultrastructural level were based on modifications of cell pellet techniques developed for peripheral blood. These earlier methods were limited in application by the number of cells in spinal fluid required to obtain a sufficient size pellet and by the lack of an easy method of cellular identification between the light and electron microscopic level. The newly developed method routinely employs microscope slides coated with Siliclad and tungsten oxide for duplicate cytocentrifuge preparations of diagnostic spinal fluid specimens. Work done by Kushida and Suzuki provided a basis for our use of the metal oxide.

A Comparative Electron Microscopic Study of Bone Repair After Internal Fracture, Osteotomy, and Perforation of Rat Tibia

Medicina ◽

10.3390/medicina49040031 ◽

2013 ◽

Vol 49 (4) ◽

pp. 31

Author(s):

Piret Hussar ◽

Ülo Hussar ◽

Inoue Kouji ◽

Tetsuji Sato

Keyword(s):

Microscopic Study ◽

Bone Repair ◽

Repair Process ◽

Inhibitory Effect ◽

Microscopic Level ◽

Electron Microscopic ◽

New Information ◽

Internal Fracture

Background and Objective. Although previous studies have provided new information on bone repair, there are still gaps in knowledge about resorptive and formative processes during bone repair at the electron microscopic level. The aim of this study was to compare bone repair after the internal fracture, osteotomy, and bicortical perforation of the tibia by means of electron microscopy. Material and Methods. An electron microscopic study of bone repair after the internal fracture, osteotomy, and bicortical perforation of the tibia was performed on 72 male Wistar rats. Rats undergoing osteotomy and perforation were further subdivided into the control and immobilization subgroups. Bone repair was observed during the first posttraumatic weeks. Results. Although bone repair in general had similar bone healing stages in all the groups, the repair process depended on the mode and degree of injury thus being different in the experimental groups. After the internal fracture, indirect ossification was observed; after osteotomy, primary periosteal, secondary endosteal ossification was noted; and after perforation, primary endosteal, secondary periosteal ossification was documented. Immobilization had an inhibitory effect on bone repair. Conclusions. The results of the present study gave new information at the electron microscopic level about intracellular changes and intercellular matrix synthesis during different types of posttraumatic bone repair and confirmed our previous reports on similar posttraumatic bone repair in histomorphometric and immunohistochemical studies.

Culture of cells in beem capsules: A new technique for electron microscopic study of monolayer cultures

In Vitro ◽

10.1007/bf02832795 ◽

1976 ◽

Vol 12 (1) ◽

pp. 65-73 ◽

Cited By ~ 5

Author(s):

John J. Eppig ◽

Edward H. Leiter ◽

Charity Waymouth

Keyword(s):

Microscopic Study ◽

New Technique ◽

Electron Microscopic ◽

Monolayer Cultures ◽

A New Technique

A specific ultrastructural method to reveal DNA: the NAMA-Ur.

Journal of Histochemistry & Cytochemistry ◽

10.1177/39.10.1719069 ◽

1991 ◽

Vol 39 (10) ◽

pp. 1427-1438 ◽

Cited By ~ 41

Author(s):

P S Testillano ◽

M A Sanchez-Pina ◽

A Olmedilla ◽

M A Ollacarizqueta ◽

C J Tandler ◽

...

Keyword(s):

Ultrastructural Level ◽

Microscopic Level ◽

Electron Microscopic ◽

En Bloc ◽

Easy Method ◽

Transmission Electron ◽

Pre Treatment ◽

Alkali Hydrolysis

We have developed a new, simple, and reproducible cytochemical method to specifically stain DNA at the electron microscopic level: the NAMA-Ur. It is based on the extraction of RNA and phosphate groups from phosphoproteins by a weak alkali hydrolysis (NA) which does not affect DNA, followed by blockage of the amino and carboxyl groups by methylation and acetylation (MA). Finally, sections are stained by uranyl (Ur), which can bind only to DNA. The efficiency of the pre-treatment (NA and MA) was demonstrated by X-ray microanalysis at the transmission electron microscopic level. The NAMA-Ur method has been successfully performed en bloc and on Lowicryl sections in mammalian and plant cells. A specific contrast is observed in the DNA-containing structures after this method, whose sensitivity allows visualization of electron-dense chromatin fibers of 10-12 nm composed of 3-nm DNA fibrils. This staining method has been combined with anti-DNA antibodies, providing complementary information to detect DNA in situ. We propose the NAMA-Ur as an easy method to investigate the chromatin organization in situ at the ultrastructural level.

The Growth of Herpesvirus mulatto in Human Fibroblast

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051232 ◽

1974 ◽

Vol 32 ◽

pp. 244-245

Author(s):

Glennelle Washington ◽

Philip P. McGrath ◽

Peter R. Graze ◽

Ivor Royston

Keyword(s):

Rhesus Monkey ◽

Microscopic Study ◽

Peripheral Blood ◽

Human Fibroblast ◽

Rhesus Monkeys ◽

Human Fibroblasts ◽

Electron Microscopic ◽

Specific Antisera ◽

Peripheral Blood Leucocytes

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065730 ◽

1971 ◽

Vol 29 ◽

pp. 338-339

Author(s):

M. J. Kramer ◽

Alan L. Coykendall

Keyword(s):

Cell Wall ◽

Dental Caries ◽

Streptococcus Mutans ◽

Microscopic Study ◽

Genetic Heterogeneity ◽

Morphological Characteristics ◽

Electron Microscopic ◽

Dna Reassociation ◽

Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

Vesicular system associated with spermiogenesis of bullfrog

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128377 ◽

1987 ◽

Vol 45 ◽

pp. 818-819

Author(s):

K. C. Liu ◽

S. F. Tsay

Keyword(s):

Electron Microscopy ◽

Microscopic Study ◽

Reproductive System ◽

Seminiferous Tubule ◽

Rana Catesbeiana ◽

Routine Procedure ◽

Electron Microscopic ◽

Twisted Tubules ◽

Electron Lucent

In the histologic and electron microscopic study of the male reproductive system of bullfrog, Rana catesbeiana, a vesicular system associated with spermiogenesis was observed. It appeared in the lumenal space of the seminiferous tubule (Fig. 1), in the heads of spermatids (Fig. 2), associated with the chromatins of the spermatid (Fig. 4). As deduced from sections, this vesicular system consisted of vesicles of various size or a large group of waving and twisted tubules (Fig. 3), After routine procedure of treatment for electron microscopy, the lumens of both of the vesicles and tubules were electron lucent.In human, vesicles and vesicular system associated with reproductive cell and tissue were reported. In abnormal spermiogenesis, flower-like body, actually vesicles, and giant vesicle associated with the head of spermatid were observed. In both cases the number of vesicle was limited from a single one to a few.

X-Ray Microanalysis of Nickel and Cobalt Intensified Peroxidase- Antiperoxidase For Verification of Reaction Sites

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119168 ◽

1985 ◽

Vol 43 ◽

pp. 468-469

Author(s):

A. Angel ◽

K. Miller ◽

V. Seybold ◽

R. Kriebel

Keyword(s):

Reaction Mechanisms ◽

Visual Discrimination ◽

Osmium Tetroxide ◽

Ultrastructural Level ◽

Microscopic Level ◽

Electron Microscopic ◽

X Ray ◽

Insoluble Polymer ◽

Cytochemical Reaction

Localization of specific substances at the ultrastructural level is dependent on the introduction of chemicals which will complex and impart an electron density at specific reaction sites. Peroxidase-antiperoxidase(PAP) methods have been successfully applied at the electron microscopic level. The PAP complex is localized by addition of its substrate, hydrogen peroxide and an electron donor, usually diaminobenzidine(DAB). On oxidation, DAB forms an insoluble polymer which is able to chelate with osmium tetroxide becoming electron dense. Since verification of reactivity is visual, discrimination of reaction product from osmiophillic structures may be difficult. Recently, x-ray microanalysis has been applied to examine cytochemical reaction precipitates, their distribution in tissues, and to study cytochemical reaction mechanisms. For example, immunoreactive sites labelled with gold have been ascertained by means of x-ray microanalysis.