Tumor Marker Expression in Breast Carcinomas and Relationship to Prognosis: An Immunohistochemical Study

Experimental pancreatic carcinoma induced in Golden Syrian Hamsters by subcutaneous injections of N-nitrosobis-(2-oxopropyl) amine (BOP) was studied during the different phases of its development by determination of serum CA 19-9 levels and tissue labeling with CA 19-9 TM. Significant differences were observed in the CA 19-9 values between the controls and animals with carcinoma (p < 0.01) and between the latter and animals with cystic or cystic papillary lesions (p < 0.01). Distribution of the deposits of CA 19-9 TM was different in the initial tumoral lesions and in the longer-developed tumors. An important correlation was observed between tissue deposits and serum levels of this tumor marker.

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Immunohistochemical study of p185 HER2 and DF3 in primary breast cancer and correlation with CA-15-3 serum tumor marker

International Journal of Gynecological Cancer ◽

10.1136/ijgc-00009577-200201000-00012 ◽

2002 ◽

Vol 12 (1) ◽

pp. 74-79

Author(s):

C Dimas ◽

M Frangos-Plemenos ◽

E Kouskouni ◽

A Kondis-Pafitis

Keyword(s):

Breast Cancer ◽

Tumor Marker ◽

Primary Breast Cancer ◽

Immunohistochemical Study ◽

Prognostic Significance ◽

Growth Factor Receptor ◽

Progesterone Receptors ◽

Her2 Overexpression ◽

Serum Tumor Marker ◽

Degree Of Differentiation

Abstract.Dimas C, Frangos-Plemenos M, Kouskouni E, Kondis-Pafitis A. Immunohistochemical study of p185 HER2 and DF3 in primary breast cancer and correlation with CA-15-3 serum tumor marker.Human epidermal growth factor receptor 2 (p185 HER2) oncoprotein immunohistochemical expression and DF3 antigen distribution were evaluated in 129 patients with primary breast cancer. p185 HER2 overexpession was positively correlated with the degree of differentiation, metastatic disease, progesterone receptors, and cytoplasmic distribution of DF3 antigen. p185 HER2 overexpression had prognostic significance for the disease-free interval.

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Immunohistochemical Study of the BCAR1/p130Cas Protein in Non-Malignant and Malignant Human Breast Tissue

The International Journal of Biological Markers ◽

10.1177/172460080101600303 ◽

2001 ◽

Vol 16 (3) ◽

pp. 172-178 ◽

Cited By ~ 12

Author(s):

S. van der Flier ◽

T.H. van der Kwast ◽

C.J.C. Claassen ◽

M. Timmermans ◽

A. Brinkman ◽

...

Keyword(s):

Breast Cancer ◽

Epithelial Cells ◽

Tumor Cells ◽

Intracellular Signaling ◽

Immunohistochemical Study ◽

Tamoxifen Treatment ◽

Breast Carcinomas ◽

Epithelial Tumor ◽

Variable Expression ◽

Malignant Breast

BCAR1/p130Cas is a docking protein involved in intracellular signaling pathways and in vitro resistance of estrogen-dependent breast cancer cells to antiestrogens. The BCAR1/p130Cas protein level in primary breast cancer cytosols was found to correlate with rapid recurrence of disease. A high BCAR1/p130Cas level was also associated with a higher likelihood of resistance to first-line tamoxifen treatment in patients with advanced breast cancer. Using antibodies raised against the rat p130Cas protein, we determined by immunohistochemical methods the BCAR1/p130Cas localization in primary breast carcinomas, in tumors of stromal origin, and in non-neoplastic breast tissues. The BCAR1/p130Cas protein was detected in the cytoplasm of non-malignant and neoplastic epithelial cells and in the vascular compartment of all tissue sections analyzed. Immunohistochemistry demonstrated variable intensity of BCAR1/p130Cas staining and variation in the proportion of BCAR1/p130Cas-positive epithelial tumor cells for the different breast carcinomas. Double immunohistochemical staining for BCAR1/p130Cas and estrogen receptor confirmed coexpression in non-malignant luminal epithelial cells and malignant breast tumor cells. The stromal cells in non-malignant tissues and tumor tissues as well as breast tumors of mesodermal origin did not stain for BCAR1/p130Cas. This immunohistochemical study demonstrates a variable expression of BCAR1/p130Cas in malignant and non-malignant breast epithelial cells, which may be of benefit for diagnostic purposes.

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