scholarly journals Clinical and analytical accuracy of a 523 gene panel next-generation sequencing (NGS) assay on formalin-fixed paraffin-embedded (FFPE) solid tumour samples

2019 ◽  
Vol 30 ◽  
pp. v575-v576
Author(s):  
I. Deras ◽  
T. Du ◽  
C. Zhao ◽  
N. Haseley ◽  
A. Yazdanparast ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Solid Tumour ◽  
Gene Panel ◽  
Next Generation ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Analytical Accuracy ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Formalin Fixed

scholarly journals Robustness of Next Generation Sequencing on Older Formalin-Fixed Paraffin-Embedded Tissue

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0127353 ◽  
Author(s):  
Danielle Mercatante Carrick ◽  
Michele G. Mehaffey ◽  
Michael C. Sachs ◽  
Sean Altekruse ◽  
Corinne Camalier ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Generation Sequencing ◽  
Formalin Fixed

scholarly journals Improved next-generation sequencing pre-capture library yields and sequencing parameters using on-bead PCR

BioTechniques ◽  
2020 ◽  
Vol 68 (1) ◽  
pp. 48-51 ◽  
Author(s):  
Christopher R McEvoy ◽  
Timothy Semple ◽  
Bhargavi Yellapu ◽  
David Y Choong ◽  
Huiling Xu ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Variant Calling ◽  
Limiting Factor ◽  
Next Generation ◽  
Tumor Biopsy ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Next Generation Sequencing Ngs ◽  
Tumor Dna ◽  
Generation Sequencing

Tumor DNA sequencing results can have important clinical implications. However, its use is often limited by low DNA input, owing to small tumor biopsy size. To help overcome this limitation we have developed a simple improvement to a commonly used next-generation sequencing (NGS) capture-based library preparation method using formalin-fixed paraffin-embedded-derived tumor DNA. By using on-bead PCR for pre-capture library generation we show that library yields are dramatically increased, resulting in decreased sample failure rates. Improved yields allowed for a reduction in PCR cycles, which translated into improved sequencing parameters without affecting variant calling. This methodology should be applicable to any NGS system in which input DNA is a limiting factor.

scholarly journals Amplicon-Based Next-Generation Sequencing for Detection of Fungi in Formalin-Fixed, Paraffin-Embedded Tissues

2020 ◽  
Vol 22 (10) ◽  
pp. 1287-1293
Author(s):  
Paige M.K. Larkin ◽  
Katy L. Lawson ◽  
Deisy A. Contreras ◽  
Catherine Q. Le ◽  
Marisol Trejo ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Generation Sequencing ◽  
Formalin Fixed
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