Sources of variation in self-incompatibility in the Australian forest tree, Eucalyptus globulus

The mating system of the animal-pollinated forest tree species Eucalyptus globulus Labill. was investigated in a clonal seed orchard to determine the importance of self-incompatibility, floral timing, floral abundance and canopy position on explaining variation in outcrossing rates. Outcrossing rates were highly and significantly correlated with the degree of self-incompatibility of individual genotypes (R2 = 0.92). Outcrossing rates were also significantly higher in the upper than in the lower parts of tree canopies (0.78 ± 0.1 and 0.54 ± 0.1, respectively). In contrast, neither flowering time nor abundance of flowers on individual trees revealed consistent effects upon outcrossing rates, suggesting that these factors are either less important or inherently more unpredictable because their effects partly depend on complex interactions with animal pollinators. Screening seed orchards in order to identify trees with high self-incompatibility and restricting seed collection to these trees will ensure that a high proportion of the seed used for plantations is derived from outcrossing, thereby minimising the deleterious effects of inbreeding depression.

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Intraspecific variation in drought susceptibility in Eucalyptus globulus is linked to differences in leaf vulnerability

Functional Plant Biology ◽

10.1071/fp18077 ◽

2019 ◽

Vol 46 (3) ◽

pp. 286 ◽

Cited By ~ 5

Author(s):

Christopher J. Lucani ◽

Timothy J. Brodribb ◽

Greg Jordan ◽

Patrick J. Mitchell

Keyword(s):

Drought Tolerance ◽

Intraspecific Variation ◽

Eucalyptus Globulus ◽

Forest Tree ◽

Species Variation ◽

Important Species ◽

Phenotypic Differences ◽

Hydraulic Failure ◽

Xylem Vulnerability ◽

Alternative Approach

Understanding intraspecific variation in the vulnerability of the xylem to hydraulic failure during drought is critical in predicting the response of forest tree species to climate change. However, few studies have assessed intraspecific variation in this trait, and a likely limitation is the large number of measurements required to generate the standard ‘vulnerability curve’ used to assess hydraulic failure. Here we explore an alternative approach that requires fewer measurements, and assess within species variation in leaf xylem vulnerability in Eucalyptus globulus Labill., an ecologically and economically important species with known genetic variation in drought tolerance. Using this approach we demonstrate significant phenotypic differences and evidence of plasticity among two provenances with contrasting drought tolerance.

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A comparative analysis of population structure of a forest tree, Eucalyptus globulus (Myrtaceae), using microsatellite markers and quantitative traits

Tree Genetics & Genomes ◽

10.1007/s11295-005-0028-7 ◽

2006 ◽

Vol 2 (1) ◽

pp. 30-38 ◽

Cited By ~ 80

Author(s):

Dorothy A. Steane ◽

Natalie Conod ◽

Rebecca C. Jones ◽

René E. Vaillancourt ◽

Bradley M. Potts

Keyword(s):

Population Structure ◽

Comparative Analysis ◽

Microsatellite Markers ◽

Eucalyptus Globulus ◽

Quantitative Traits ◽

Forest Tree

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Partitioning and distribution of RAPD variation in a forest tree species, Eucalyptus globulus (Myrtaceae)

Heredity ◽

10.1038/hdy.1995.86 ◽

1995 ◽

Vol 74 (6) ◽

pp. 628-637 ◽

Cited By ~ 78

Author(s):

K A Nesbitt ◽

B M Potts ◽

R E Vaillancourt ◽

A K West ◽

J B Reid

Keyword(s):

Tree Species ◽

Eucalyptus Globulus ◽

Forest Tree ◽

Forest Tree Species ◽

Rapd Variation

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Self-incompatibility in Eucalyptus globulus ssp. globulus (Myrtaceae)

Australian Journal of Botany ◽

10.1071/bt01076 ◽

2002 ◽

Vol 50 (3) ◽

pp. 365 ◽

Cited By ~ 33

Author(s):

L. M. Pound ◽

M. A. B. Wallwork ◽

B. M. Potts ◽

M. Sedgley

Keyword(s):

Fluorescence Microscopy ◽

Seed Production ◽

Eucalyptus Globulus ◽

Pollen Tubes ◽

The Self ◽

Self Incompatibility ◽

Seed Maturity ◽

Significant Difference ◽

Site Of Action ◽

Ovule Penetration

Controlled pollinations with self- and cross-pollen were applied to individual flowers of five mature Eucalyptus globulus Labill. ssp. globulus trees to investigate the site of action of the self-incompatibility mechanism. Growth of pollen tubes in styles at 2 weeks after pollination and ovule penetration by pollen tubes at 2 and 4 weeks after pollination were investigated by fluorescence microscopy. Some pollinated flowers were left to develop to seed maturity, then harvested to quantify the level of self-incompatibility of each tree. Trees ranged from 76 to 100% self-incompatible. There was no significant difference in the number of pollen tubes in the style between treatments although variation was present between trees. The number of pollen tubes present was similar to the number of ovules present within flowers. Penetration of ovules by pollen tubes over all five trees combined revealed no difference between treatments at 2 weeks after pollination; however, there was slightly greater penetration by cross-pollen tubes at 4 weeks after pollination. This difference was not large enough to account for the near complete lack of selfed-seed production, suggesting late pre- or post-zygotic arrest of selfed ovules.

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Sources of variation in foliar secondary chemistry in a tropical forest tree community

Ecology ◽

10.1002/ecy.1689 ◽

2017 ◽

Vol 98 (3) ◽

pp. 616-623 ◽

Cited By ~ 38

Author(s):

Brian E. Sedio ◽

Juan C. Rojas Echeverri ◽

Cristopher A. Boya P. ◽

S. Joseph Wright

Keyword(s):

Tropical Forest ◽

Forest Tree ◽

Secondary Chemistry ◽

Tree Community ◽

Sources Of Variation

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Somatic Embryogenesis as a tool for forest tree improvement: a case- study in Eucalyptus globulus

BMC Proceedings ◽

10.1186/1753-6561-5-s7-p128 ◽

2011 ◽

Vol 5 (S7) ◽

Cited By ~ 4

Author(s):

Gisele Andrade ◽

Ravi Shah ◽

Sofie Johansson ◽

Gloria Pinto ◽

Ulrika Egertsdotter

Keyword(s):

Somatic Embryogenesis ◽

Eucalyptus Globulus ◽

Forest Tree ◽

Tree Improvement

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Self-sterility May Be Due to Prezygotic Late-acting Self-incompatibility and Early-acting Inbreeding Depression in Chinese Chestnut

Journal of the American Society for Horticultural Science ◽

10.21273/jashs04634-18 ◽

2019 ◽

Vol 144 (3) ◽

pp. 172-181 ◽

Cited By ~ 2

Author(s):

Huan Xiong ◽

Feng Zou ◽

Sujuan Guo ◽

Deyi Yuan ◽

Genhua Niu

Keyword(s):

Inbreeding Depression ◽

Fruit Set ◽

Paraffin Section ◽

Forest Tree ◽

The Self ◽

Self Incompatibility ◽

Double Fertilization ◽

Chinese Chestnut ◽

Section Technique ◽

Ovule Penetration

Chinese chestnut (Castanea mollissima), which is native to China, has been cultivated as a nontimber forest tree species for 4000 years. This species has been found to display self-sterility, which results in a significantly lower seed set following self-pollination (SP) compared with that following cross-pollination (CP). Self-sterility can be induced by prezygotic or postzygotic late-acting self-incompatibility (LSI) or early-acting inbreeding depression (EID). To elucidate the causes of self-sterility in chestnut, we investigated pollen–pistil interactions, fertilization, and early ovule development following SP and CP by using a paraffin section technique and fluorescence microscopy. The fruit set percentage and seed characteristics also were evaluated among different pollination treatments. The results show that there were no significant differences in pollen tube behavior following SP vs. CP, regardless of the stigmatic or stylar level. Double fertilization was significantly greater following CP (18.09%) than SP (2.58%). The significantly lower percentages of ovule penetration and double fertilization in the selfed vs. crossed ovules support a prezygotic LSI mechanism in C. mollissima. The fruit set resulting from chase-pollination (CHP; 53.85% to 63.64%) was greater than that resulting from SP (12.12% to 14.00%). In addition, the distribution of aborted seed sizes after SP showed a widely clumped pattern. Abortion occurred at different stages during seed development rather than at a uniform stage, which supported the idea that EID was operating in C. mollissima. Levels of self-sterility in the chinese chestnut trees ranged from 88.2% to 90.5%. Thus, partial prezygotic LSI and EID contributed to self-sterility in the C. mollissima ‘Yanshanzaofeng’, with prezygotic LSI rejecting part of the self-pollen in the ovary and EID aborting part of the self-fertilized seeds.

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