scholarly journals Epigenetic imbalance and the floral developmental abnormality of the in vitro-regenerated oil palm Elaeis guineensis

2011 ◽  
Vol 108 (8) ◽  
pp. 1453-1462 ◽  
Author(s):  
Estelle Jaligot ◽  
Sophie Adler ◽  
Émilie Debladis ◽  
Thierry Beulé ◽  
Frédérique Richaud ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Developmental Abnormality

scholarly journals Accelerated in vitro propagation of elite oil palm genotypes (Elaeis guineensis Jacq.) by substituting cytokinin with putrescine

2016 ◽  
Vol 15 (50) ◽  
pp. 2767-2775 ◽  
Author(s):  
Roseli Correa Thais ◽  
Yoshimitsu Motoike Sérgio ◽  
Paula de Souza Andrade Ana ◽  
Morra Coser Sara ◽  
Queiroz Vanessa ◽  
...  
Keyword(s):  
Oil Palm ◽  
In Vitro Propagation ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

A modeling approach of the in vitro conversion of oil palm (Elaeis guineensis) somatic embryos

2005 ◽  
Vol 84 (1) ◽  
pp. 100-113 ◽  
Author(s):  
Eugéne E. Konan ◽  
Tristan Durand-Gasselin ◽  
Justin Y. Kouadio ◽  
Albert Flori ◽  
Alain Rival
Keyword(s):  
Oil Palm ◽  
Somatic Embryos ◽  
Elaeis Guineensis ◽  
Modeling Approach

Evidence for an interaction effect during in vitro rooting of oil palm (Elaeis guineensis Jacq.) somatic embryo-derived plantlets

2007 ◽  
Vol 43 (5) ◽  
pp. 456-466 ◽  
Author(s):  
Eugène K. Konan ◽  
Justin Y. Kouadio ◽  
Albert Flori ◽  
Tristan Durand-Gasselin ◽  
Alain Rival
Keyword(s):  
Somatic Embryo ◽  
Interaction Effect ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
In Vitro Rooting ◽  
Elaeis Guineensis Jacq

In vitro human gastro-intestinal enzyme digestibility of globulin isolate from oil palm (Elaeis guineensis var. tenera) kernel meal and the bioactivity of the digest

RSC Advances ◽  
2016 ◽  
Vol 6 (24) ◽  
pp. 20219-20229 ◽  
Author(s):  
Arun Tapal ◽  
Gerd E. Vegarud ◽  
Ashoka Sreedhara ◽  
Prajna Hegde ◽  
Shashikala Inamdar ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Ex Vivo ◽  
Palm Kernel ◽  
Kernel Meal ◽  
Intestinal Enzyme ◽  
Enzyme Digestibility

Theex vivohydrolysate of oil palm kernel globulin isolate exhibit bioactivity.

scholarly journals Pembentukan akar in vitro planlet kelapa sawit (Elaeis guineensis Jacq.) dalam medium cair dengan penambahan auksin In vitro rooting of oil palm (Elaeis guineensis Jacq.) plantlets in a liquid medium supplemented with auxins

2016 ◽  
Vol 78 (1) ◽  
Author(s):  
Imron RIYADI ◽  
. SUMARYONO
Keyword(s):  
Liquid Medium ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Root Induction ◽  
Material Source ◽  
Shoot Height ◽  
Aerial Parts ◽  
Elaeis Guineensis Jacq ◽  
Root Quality

AbstractAuxin affects the growth and development of in vitro plantlets including root induction. An experiment was conducted to determine the combination and concentration of auxin for rooting of oil palm plantlets in liquid medium.Unrooted plantlets of oil palm MK 649 clone with height 6 – 7 cm and 2 – 3 leaves were used as material source. The plantlets were cultured in de Fossard liquid medium. The treatments used were combinations of NAA and IBA at 0, 5,10 and 20 μM. The results show that 10 μM NAA combined with 20 μM IBA gave the highest percentage of rooting of oil palm plantlets (73.3%) in 10 weeks. NAA and IBA concentration influenced significantly rooting percentageand root quality and there was a significant interaction between the two auxins. Root initiation response of oil palm plantlets to NAA was higher than to IBA. The best of oil palm root class which indicates root quality was obtained in a medium with 10 μM NAA + 20 μM IBA. The aerial parts of the plantlets grew well in term of shoot height, leaf number and shoot diameter especially in a medium with 10 μM NAA + 20 μM IBA. AbstrakAuksin berpengaruh terhadap pertumbuhan dan perkembangan planlet in vitro, termasuk terhadap induksi akar. Penelitian ini bertujuan untuk menentukan kombinasi dan konsentrasi auksin yang tepat dalam pembentukan akarplanlet kelapa sawit in vitro dalam medium cair. Bahan yang digunakan berupa planlet kelapa sawit klon MK 649 tanpa akar dengan tinggi 6 – 7 cm dan jumlah daun 2 – 3 helai. Planlet dikulturkan dalam medium de Fossard cair. Perlakuan yang digunakan adalah kombinasi NAA dan IBA dengan konsentrasi 0, 5, 10 dan 20 μM. Hasil penelitian menunjukkan bahwa perlakuan NAA 10 μM dikombinasikan dengan IBA 20 μM menghasilkan persentase pembentukan akar planlet kelapa sawit tertinggi yaitu 73,3% dalam waktu 10 minggu. Konsentrasi NAA dan IBA secara nyata mempengaruhi persentase pembentukan dan kualitas akar serta terdapat interaksi yang nyata antara kedua perlakuan auksin. Respons induksi akar kelapa sawit terhadap NAA lebih tinggi daripada IBA. Kelas akar planlet kelapa sawit terbaik yang menunjukkan kualitas perakaran, juga diperoleh pada NAA 10 μM dan IBA 20 μM. Pertumbuhan dan perkembangan organ bagian atas yang meliputi tinggi tunas, jumlah daun dan diameter tunas menunjukkan peningkatan yang cukup baik terutama pada perlakuan NAA 10 μM + IBA 20 μM.

scholarly journals In Vitro Wound Healing Potential of Flavonoid C-Glycosides from Oil Palm (Elaeis guineensis Jacq.) Leaves on 3T3 Fibroblast Cells

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 326 ◽  
Author(s):  
Mohamad Shazeli Che Zain ◽  
Soo Yee Lee ◽  
Murni Nazira Sarian ◽  
Sharida Fakurazi ◽  
Khozirah Shaari
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Fibroblast Cells ◽  
Crude Extracts ◽  
Elaeis Guineensis Jacq ◽  
And Migration ◽  
Proliferation And Migration

Oil palm (Elaeis guineensis Jacq.) leaves (OPL) are widely available at zero cost in Southeast Asia countries, especially in Malaysia and Indonesia due to large scale oil palm plantations. OPLs contain a large amount of flavonoids in particular flavonoid C-glycosides, which are known to possess useful biological properties including antioxidant and wound healing properties. The present study aimed at evaluating the wound healing efficacy of OPL in various solvent extracts and flavonoid enriched fractions and to determine the contribution of flavonoid C-glycosides (orientin, isoorientin, vitexin and isovitexin) using in-vitro scratch assay on 3T3 fibroblast cells. Solvent crude extracts with different polarity were screened and the most active extract was subjected to acid hydrolysis. The crude and acid hydrolysed extracts were further enriched using macroporous resins, XAD7HP. UHPLC-UV/PDA and LC-MS/MS analysis were applied for identification and confirmation of flavonoid C-glycosides. The wound healing properties comprised of cell viability, cell proliferation and cell migration were studied. Allantoin was used as a positive control to compare the efficacy among the tested samples. The results revealed all OPL crude extracts, flavonoid enriched fractions and flavonoid C-glycosides were non-toxic at concentrations below 25 µg/mL and showed better cell proliferation and migration activities at low concentrations than higher concentrations.. This study also demonstrated orientin, isoorientin, vitexin and isovitexin presented in OPL extracts and flavonoid enriched fractions stimulated proliferation and migration of 3T3 fibroblast cells. Hence, these findings may pose potential therapeutic bioactive agents for wound healing by enhancing fibroblast proliferation and migration.

INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ

2021 ◽  
Vol 45 ◽  
Author(s):  
Marlúcia Souza Pádua Vilela ◽  
Jéssica de Castro e Andrade ◽  
Raíssa Silveira Santos ◽  
Vanessa Cristina Stein ◽  
Patrick Callegari Magnani Santos Alves ◽  
...  
Keyword(s):  
Oil Palm ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Callus Formation ◽  
Leaf Explants ◽  
Dichlorophenoxyacetic Acid ◽  
In Vitro Cultivation ◽  
Low Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid

ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oil palm plants in low concentrations of auxins and to observe the maintenance of these characteristics during in vitro cultivation. Calluses were induced in 0.5 cm leaf explants in Y3 culture medium supplemented with Picloram (4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) or 2,4-D (2,4-dichlorophenoxyacetic acid), at concentrations of 0, 1, 3, 6, and 9 mg L-1. The callus with embryogenic appearance was subcultured and evaluated regarding maintenance of embryogenic characteristics by cytochemical analyses. The best treatment for induction of calluses was composed of 1mg.L-1 of Picloram, which led to 30% callus formation. The calluses were classified into4 types, based on color and morphology. The cells of calluses with nodular and beige appearance have embryogenic characteristics, and the embryogenic potential of the cell masses was maintained over the 20 months of cultivation. This differentiated adaptation to the protocol can allow the advance in the mass propagation of oil palm through tissue culture, indicating the importance of investigating the topics proposed by the research.

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