scholarly journals Antiapoptotic activity of methyl (3R,4E,6Z,15E)-3-hydroxyoctadecatrienoate in human cervical carcinoma HeLa cells

2021 ◽  
Vol 85 (2) ◽  
pp. 228-232
Author(s):  
Osamu Ohno ◽  
Keigo Sato ◽  
Ryo Honma ◽  
Ruri Chiba ◽  
Kenji Matsuno
Keyword(s):  
Cell Death ◽  
Cervical Carcinoma ◽  
Column Chromatography ◽  
Hela Cells ◽  
Active Compound ◽  
Inhibitory Activity ◽  
Marine Organisms ◽  
Meoh Extract ◽  
Human Cervical Carcinoma ◽  
Antiapoptotic Activity

ABSTRACT Inhibitors of thapsigargin-induced cell death in human cervical carcinoma HeLa cells were screened among the metabolites of marine organisms. The MeOH extract of the cyanobacterium Rivularia sp. was found to exhibit inhibitory activity. Column chromatography purification was used to isolate methyl (3R,4E,6Z,15E)-3-hydroxyoctadecatrienoate (MHO) as the active compound. MHO was determined to inhibit apoptotic stimuli-induced cell death in HeLa cells.

Yuk-Hap-Tang Induces Apoptosis by Intervening Mn-SOD in Human Cervical Carcinoma HeLa Cells

2004 ◽  
Vol 32 (06) ◽  
pp. 883-895 ◽  
Author(s):  
H. J. Chae ◽  
J. M. Park ◽  
G. Y. Lee ◽  
H. R. Park ◽  
S. W. Chae ◽  
...  
Keyword(s):  
Cell Death ◽  
Cervical Carcinoma ◽  
Anticancer Agents ◽  
Hela Cells ◽  
Caspase 3 ◽  
Reduced Glutathione ◽  
Cleavage Product ◽  
P53 Expression ◽  
Human Cervical Carcinoma ◽  
Apoptotic Protein

Yuk-Hap-Tang (YHT) induces cell death in human cervical carcinoma HeLa cells. Caspase-3, -6 and -9 were markedly activated in HeLa cells treated with YHT. The preferred substrate for caspase-3 cysteine protease, PARP, was cleaved to its 85-kDa cleavage product. YHT increased the amount of the anti-apoptotic protein, Bcl-2, and the pro-apoptotic protein, Bax. Although p53 has been reported to accumulate in cancer cells in response to anticancer agents, the p53 expression level was not changed in HeLa cells treated with YHT. Manganese (Mn)-TBAP, a mitochondria-specific SOD mimetic agent and NAC/GSH (N-acetyl cysteine/reduced glutathione) reduced the YHT-induced cytotoxicity and decreased the number of the YHT-induced apoptotic cells. Furthermore, YHT reduced the expression of Mn-SOD protein and its activity in HeLa cells. The data demonstrate that YHT induces the apoptosis of human cervical carcinoma HeLa cells by intervening Mn-SOD.

Apoptosis induction by α-mangostin-loaded nanoparticles in human cervical carcinoma cells

2020 ◽  
Vol 75 (5-6) ◽  
pp. 145-151
Author(s):  
Minh H. Nguyen ◽  
Dat T. Nguyen ◽  
Phuong T.M. Nguyen
Keyword(s):  
Cell Death ◽  
Cervical Carcinoma ◽  
Hela Cells ◽  
Free Form ◽  
Apoptosis Induction ◽  
Garcinia Mangostana ◽  
Anticancer Properties ◽  
Human Cervical Carcinoma ◽  
Late Apoptosis ◽  
New Formula

AbstractThe compound α-mangostin (AMG) extracted from Garcinia mangostana L. has potent anticancer properties but its clinical application is limited because of its poor solubility. In this study, AMG-loaded nanoparticles (NMG) were synthesized using a new formula and their apoptosis activity against human cervical carcinoma (HeLa) cells was investigated in comparison with organic solvent-soluble AMG in free form. The NMG was successfully synthesized with a particle size of <50 nm, polydispersity index <0.3, and zeta potential of −35.2 mV. At a concentration of 10 μg/mL, AMG reduced cell survival by 60%, whereas NMG treatment resulted in >90% cell death (p < 0.05). The AMG- or NMG-treated cells also showed changes in the size and shape and exhibited enhanced intensity of blue-stained nuclei, as well as decreased cell density, especially in NMG-treated cells. After 24 h of incubation with AMG or NMG, the cells went through late apoptosis at a rate of approximately 34% in 20 μg/mL AMG treatment and 27% in 10 μg/mL NMG treatment (p < 0.05). Thus, HeLa cells underwent more pronounced cell death through apoptosis induction caused by the NMG treatment compared to that caused by AMG. Clearly, the new NMG improved AMG bioavailability while maintaining the desired activity.

Design, synthesis, and antitumor activity of novel paeonol derivatives containing the 1,4-benzoxazinone and 1,2,3-triazole moieties

2019 ◽  
Vol 43 (7-8) ◽  
pp. 241-247
Author(s):  
Tingting Yang ◽  
Xin Shi ◽  
Libing Guo ◽  
Shaohua Gu ◽  
Weiwei Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cervical Carcinoma ◽  
Cell Lung Cancer ◽  
Hela Cells ◽  
Inhibitory Activity ◽  
Small Cell ◽  
Small Cell Lung ◽  
Human Cervical Carcinoma

A new series of paeonol derivatives containing the 1,4-benzoxazinone and 1,2,3-triazole moieties were synthesized and evaluated for their cytotoxicity in vitro against human non-small cell lung cancer NCI-H1299 cells and human cervical carcinoma HeLa cells. Among them, compared with that of paeonol, compounds 8-acetyl-4-{[(1-(5-chloro-2-nitrophenyl)-1 H-1,2,3-triazol-4-yl]methyl}-5-methoxy-2 H-1,4-benzoxazin-3(4 H)-one, 8-acetyl-4-[(1-mesityl-1 H-1,2,3-triazol-4-yl)methyl]-5-methoxy-2 H-1,4-benzoxazin-3(4 H)-one, and 8-acetyl-5-methoxy-4-{[(1-(naphthalen-1-yl)-1 H-1,2,3-triazol-4-yl]methyl}-2 H-1,4-benzoxazin-3(4 H)-one exhibited significant inhibitory activity toward the human non-small cell lung cancer NCI-H1299 cells (IC50 = 13.36 ± 0.003, 19.75 ± 0.3, 15.79 ± 0.05 μg mL−1). The last compound also exhibited significant inhibitory activity toward the human cervical carcinoma HeLa cells (IC50 = 19.73 ± 1.0 μg mL−1).

scholarly journals Cytotoxic activity of proteins isolated from extracts of Corydalis cava tubers in human cervical carcinoma HeLa cells

2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Robert Nawrot ◽  
Maria Wolun-Cholewa ◽  
Wojciech Bialas ◽  
Danuta Wyrzykowska ◽  
Stanislaw Balcerkiewicz ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cervical Carcinoma ◽  
Hela Cells ◽  
Human Cervical Carcinoma

Wogonin induces G1 phase arrest through inhibiting Cdk4 and cyclin D1 concomitant with an elevation in p21Cip1 in human cervical carcinoma HeLa cells

2009 ◽  
Vol 87 (6) ◽  
pp. 933-942 ◽  
Author(s):  
Li Yang ◽  
Hai-wei Zhang ◽  
Rong Hu ◽  
Yong Yang ◽  
Qi Qi ◽  
...  
Keyword(s):  
Cyclin D1 ◽  
Cervical Carcinoma ◽  
Hela Cells ◽  
Therapeutic Potential ◽  
P53 Gene ◽  
Mrna Levels ◽  
Human Cervical Carcinoma ◽  
Phase Arrest

Wogonin, a naturally occurring flavonoid, has been shown to have tumor therapeutic potential both in vitro and in vivo. To better understand its anticancer mechanism, we examined the effect of wogonin on human cervical carcinoma HeLa cells. In this study, we observed that G1 phase arrest was involved in wogonin-induced growth inhibition in HeLa cells. Over a 24 h exposure of HeLa cells to 90 µmol·L–1 wogonin, the promoters of G1–S transition, including cyclin D1/Cdk4 and pRb, decreased within 12 h and E2F-1 depleted in the nucleus at the same time. As the G1 phase arrest developed, p53 and the Cdk inhibitor p21Cip1 elevated both at protein and mRNA levels. Furthermore, the up-regulation of p21Cip1 induced by wogonin was dramatically inhibited by siRNA-mediated p53 gene silencing. Collectively, our data suggested that wogonin induced G1 phase arrest in HeLa cells by modulating several key G1 regulatory proteins, such as Cdk4 and cyclin D1, as well as up-regulation of a p53-midiated p21Cip1 expression. This mechanism of wogonin may play an important role in the killing of cancerous cells and offer a potential mechanism for its anticancer action in vivo.

P53-mediated GSH depletion enhanced the cytotoxicity of NO in silibinin-treated human cervical carcinoma HeLa cells

2012 ◽  
Vol 46 (9) ◽  
pp. 1082-1092 ◽  
Author(s):  
Simiao Fan ◽  
Yang Yu ◽  
Min Qi ◽  
Zhongdong Sun ◽  
Lihua Li ◽  
...  
Keyword(s):  
Cervical Carcinoma ◽  
Hela Cells ◽  
Human Cervical Carcinoma

Effect of aspirin alone or combined with cisplatin on human cervical carcinoma HeLa cells

2010 ◽  
Vol 25 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Wang Yueling ◽  
Zhao Hongmin ◽  
Liu Lin ◽  
Wang Jiangfen
Keyword(s):  
Cervical Carcinoma ◽  
Hela Cells ◽  
Human Cervical Carcinoma
Sign in / Sign up

Export Citation Format

Share Document