Tenuazonic acid production is dispensable for virulence, but its biosynthetic gene expression pattern is associated with the infection of Pyricularia oryzae

2021 ◽  
Author(s):  
Takeshi Kashiwa ◽  
Takayuki Motoyama ◽  
Kazuko Yoshida ◽  
Choong-Soo Yun ◽  
Hiroyuki Osada
Keyword(s):  
Gene Expression ◽  
Expression Pattern ◽  
Acid Production ◽  
Rice Blast ◽  
Gene Expression Pattern ◽  
Rice Blast Fungus ◽  
Pyricularia Oryzae ◽  
Biosynthetic Gene ◽  
Tenuazonic Acid ◽  
Biosynthetic Gene Expression

Abstract Tenuazonic acid (TeA) is a toxin produced by the rice blast fungus Pyricularia oryzae. Although knockout of the TeA biosynthetic gene TAS1 did not affect the virulence of P. oryzae, constitutive TAS1 expression suppressed its infection. TAS1 expression was induced alongside transition of P. oryzae infection behavior. The results suggested that controlling TeA biosynthesis is important for P. oryzae infection.

scholarly journals Antifungal Activity of Eucalyptus Oil against Rice Blast Fungi and the Possible Mechanism of Gene Expression Pattern

Molecules ◽  
2016 ◽  
Vol 21 (5) ◽  
pp. 621 ◽  
Author(s):  
Li-Jun Zhou ◽  
Fu-Rong Li ◽  
Li-Jie Huang ◽  
Zhi-Rong Yang ◽  
Shu Yuan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Antifungal Activity ◽  
Expression Pattern ◽  
Rice Blast ◽  
Gene Expression Pattern ◽  
Eucalyptus Oil

scholarly journals Mycovirus-Induced Tenuazonic Acid Production in a Rice Blast Fungus Magnaporthe oryzae

2020 ◽  
Vol 11 ◽  
Author(s):  
Akihiro Ninomiya ◽  
Syun-ichi Urayama ◽  
Rei Suo ◽  
Shiro Itoi ◽  
Shin-ichi Fuji ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Acid Production ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Tenuazonic Acid ◽  
Blast Fungus

scholarly journals Elevation of gene expression of Btg2, Gadd 153, and antioxidant markers in RONS-induced PC12 cells

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Aravind P ◽  
Sarojini R. Bulbule ◽  
Hemalatha N ◽  
Anushree G ◽  
Babu R.L ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endoplasmic Reticulum ◽  
Protein Expression ◽  
Pc12 Cells ◽  
Expression Pattern ◽  
High Glucose ◽  
Nitrosative Stress ◽  
Gene Expression Pattern ◽  
Marker Genes ◽  
Differential Stress

Abstract Background Free radicals generated in the biological system bring about modifications in biological molecules causing damage to their structure and function. Identifying the damage caused by ROS and RNS is important to predict the pathway of apoptosis due to stress in PC12 cells. The first defense mechanisms against them are antioxidants which act in various pathways through important cellular organelles like the mitochondria and endoplasmic reticulum. Specific biomarkers like Gadd153 which is a marker for endoplasmic reticulum stress, Nrf2 which responds to the redox changes and translocates the antioxidant response elements, and Btg2 which is an antioxidant regulator have not been addressed in different stress conditions previously in PC12 cells. Therefore, the study was conducted to analyze the gene expression pattern (SOD, Catalase, Btg2, Gadd153, and Nrf2) and the protein expression pattern (iNOS and MnSOD) of the antioxidant stress markers in differential stress-induced PC12 cells. Peroxynitrite (1 μM), rotenone (1 μM), H2O2(100 mM), and high glucose (33 mM) were used to induce oxidative and nitrosative stress in PC12 cells. Results The results obtained suggested that rotenone-induced PC12 cells showed a significant increase in the expression of catalase, Btg2, and Gadd153 compared to the control. Peroxynitrite-induced PC12 cells showed higher expression of Btg2 compared to the control. H2O2 and high glucose showed lesser expression compared to the control in all stress marker genes. In contrast, the Nrf2 gene expression is downregulated in all the stress-induced PC12 cells compared to the control. Further, MnSOD and iNOS protein expression studies suggest that PC12 cells exhibit a selective downregulation. Lower protein expression of MnSOD and iNOS may be resulted due to the mitochondrial dysfunction in peroxynitrite-, high glucose-, and H2O2-treated cells, whereas rotenone-induced cells showed lower expression, which could be the result of a dysfunction of the endoplasmic reticulum. Conclusion Different stress inducers like rotenone, peroxynitrite, H2O2, and high glucose increase the NO and ROS. Btg2 and Gadd153 genes were upregulated in the stress-induced cells, whereas the Nrf2 was significantly downregulated in differential stress-induced PC12 cells. Further, antioxidant marker genes were differentially expressed with different stress inducers.

CNS gene expression pattern associated with spontaneous experimental autoimmune encephalomyelitis

2003 ◽  
Vol 73 (5) ◽  
pp. 667-678 ◽  
Author(s):  
Agata Matejuk ◽  
Corwyn Hopke ◽  
Jami Dwyer ◽  
Sandhya Subramanian ◽  
Richard E. Jones ◽  
...  
Keyword(s):  
Gene Expression ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Autoimmune Encephalomyelitis ◽  
Experimental Autoimmune
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