Developmental Exposures of Male Rats to Bisphenol A (BPA) Decrease Serum Adiponectin Levels and Expression of Adiponectin Receptors in Leydig Cells in Association with Inhibition of Androgen Biosynthesis.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 306-306
Author(s):  
Manjunatha K. Nanjappa ◽  
Benson T. Akingbemi
Keyword(s):  
Bisphenol A ◽  
Leydig Cells ◽  
Male Rats ◽  
Serum Adiponectin ◽  
Adiponectin Receptors ◽  
Developmental Exposures

scholarly journals Мorphofunctional Characteristics of the Male Reproductive System of White Rats Treated with Bisphenol A and Triclosan

2019 ◽  
Vol 8 (1) ◽  
pp. 77-81
Author(s):  
S. V. Chigrinets ◽  
G. V. Bryukhin ◽  
S. N. Zav'yalov
Keyword(s):  
Bisphenol A ◽  
Leydig Cells ◽  
Statistical Processing ◽  
Mature Male ◽  
Male Rats ◽  
Total Count ◽  
White Rats ◽  
Comparison Groups ◽  
Negative Effect ◽  
Sexually Mature

The aimof this study was to analyze the morphofunctional state of the testes of mature male rats treated with bisphenol A (BPA) and triclosan (TCS).Material and methods.The work was performed on mature male rats (n=28). Experimental animals were divided into three groups – control (intact) and two experimental ones. For two months, rats of the experimental groups received daily bisphenol A and triclosan (Sigma-Aldrich, USA) with food in the amount of 200 mg/kg. The total count of spermatozoa in 1 ml of sperm was determined with the calculation of their atypical forms, and morphometric measurements were made (the total number and area of Leydig cells with their nuclearcytoplasmic ratio). The concentrations of bisphenol A and testicular triclosan were measured by gas chromatography with mass spectrometry (GC-MS). The obtained data was subjected to statistical processing using IBM SPSS Statistics v.21 (IBM Corp., Armonk, NY, USA).Results.Differences between the comparison groups in the concentration of bisphenol A and triclosan in testicular tissues were statistically significant (p <0.001). The endocrine disruptors studied reduced the mass of the testes. A group of male rats exposed to bisphenol A showed a decrease in the total number of spermatozoa (p=0.004) with an increase in their atypical forms (p=0.014) compared with a group of intact animals. Bisphenol A and triclosan caused a decrease in the total number of Leydig cells (p=0.001; p=0.001) respectively, and a statistically significant change in the nuclear-cytoplasmic ratio. Moreover, bisphenol A led to a decrease in the nuclear-cytoplasmic ratio of Leydig cells, whereas triclosal, on the contrary, increased its value in comparison with a group of intact animals.Conclusion.Bisphenol A and triclosan have a negative effect on the morphofunctional state of the male testes of sexually mature rats (decrease in testicular mass, total count of spermatozoa on the background of an increase in their atypical forms, as well as a decrease in the total number of Leydig cells with a change in their nuclear-cytoplasmic ratio).

scholarly journals Bisphenol A Down-Regulates The mRNA Expression of Steroidogenic Genes and Induces Histopathological Changes in Testes Of Rats

2018 ◽  
Vol 1 (3) ◽  
pp. 32-44
Author(s):  
Basma H Marghani ◽  
Ahmed I Ateya ◽  
Rasha M Saleh ◽  
Amal A Halawa ◽  
Rasha A Eltaysh ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Mrna Expression ◽  
Leydig Cells ◽  
Expression Patterns ◽  
Regulatory Protein ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Steroid Synthesis ◽  
Steroidogenic Genes

Bisphenol A (BPA) is an endocrine disruptor with a weak estrogenic effect used in industry as a component of food cans. We aimed to study the toxic effects of BPA on mRNA expression of steroidogenic genes and testicular structure in mature male rats. Animals were divided into 3 groups: vehicle control rats as first group, while second group received 10 µg/kg BW and third group received BPA 15 µg/kg BW orally every alternate day for a period of 105 successive days. Serum testosterone level, mRNA expression of genes related to steroid synthesis, histopathological examination, spermatogenesis index and number of Leydig cells were evaluated in this study. Lower serum hormone levels were observed in both BPA-treated groups as compared to the control group. The gene expression patterns of steroidogenic acute regulatory protein (StAR), cytochrome P450 17a(CYP17a) and 3β-Hydroxysteroid dehydrogenase (3β-HSD) were significantly down-regulated in BPA-treated rats compared to control group. Meanwhile, the expression of aromatase (CYP19) and lutinizing hormone receptor (LHR) was significantly up-regulated. Histopathological lesions were observed in the testes and epididymis of BPA-treated rats. Spermatogenesis index and the number of Leydig cells were significantly decreased in BPA-treated groups compared with the control group. This study highlights negative effect of BPA on steroidogenic genes and testicular structure in male rats.

scholarly journals Behavioral Alterations in Response to Fear-Provoking Stimuli and Tranylcypromine Induced by Perinatal Exposure to Bisphenol A and Nonylphenol in Male Rats

2004 ◽  
Vol 112 (11) ◽  
pp. 1159-1164 ◽  
Author(s):  
Takayuki Negishi ◽  
Katsuyoshi Kawasaki ◽  
Shingo Suzaki ◽  
Haruna Maeda ◽  
Yoshiyuki Ishii ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Male Rats ◽  
Perinatal Exposure ◽  
Behavioral Alterations

Bisphenol A attenuates testosterone production in Leydig cells via the inhibition of NR1D1 signaling

Chemosphere ◽  
2021 ◽  
Vol 263 ◽  
pp. 128020 ◽  
Author(s):  
Cuimei Li ◽  
Linlin Zhang ◽  
Tiantian Ma ◽  
Lei Gao ◽  
Luda Yang ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Leydig Cells ◽  
Testosterone Production

Investigation of the effects of bisphenol-A exposure on lymphoid system in prenatal stage

2020 ◽  
Vol 36 (7) ◽  
pp. 502-513
Author(s):  
Işil Aydemir ◽  
Caner Özbey ◽  
Oktay Özkan ◽  
Şadiye Kum ◽  
Mehmet İbrahim Tuğlu
Keyword(s):  
Lymph Node ◽  
Bisphenol A ◽  
Tissue Damage ◽  
Corn Oil ◽  
Histopathological Examination ◽  
Female Rats ◽  
Male Rats ◽  
High Dose ◽  
Organ Function ◽  
Pregnant Rats

Bisphenol-A (BPA) used in the production of plastic materials is a temperature-soluble agent. It also has a steroid hormone-like activity; therefore, it poses a danger to human health. In our study, we aimed to investigate the effects of BPA on lymph node and spleen in male rats exposed to this agent during prenatal stage. The pregnant female rats were divided into four groups: control, sham, low dose (300 µg/kg BPA), and high dose (900 µg/kg BPA). BPA was dissolved in 1 mL of corn oil and administered to the pregnant rats every day during pregnancy. On the 21st and 45th day after the birth, male rats’ lymph node and spleen samples were taken and histopathological examination was performed. Samples were stained with hematoxylin and eosin to determine the general histological appearance, and with CD3 and CD20 immunohistochemically. The results of staining were evaluated by H-score, and statistical analysis was performed. In the samples, BPA applications were not found to cause significant tissue damage. But there was a significant decrease in the immunoreactivities of CD3 and CD20 after BPA applications in both 21st and 45th day samples. After high dose BPA administration, decreased CD3 immunoreactivity was statistically significant. It is thought that BPA does not cause histologically significant tissue damage, but it may impair organ function at cellular level. The investigation of molecules involved in organ function will be useful in revealing the mechanisms that will cause dysfunction.

"Bisphenol-A impairs memory and reduces dendritic spine density in adult male rats": Correction to Eilam-Stock et al. (2011).

2012 ◽  
Vol 126 (1) ◽  
pp. 195-195
Author(s):  
Tehila Eilam-Stock ◽  
Peter Serrano ◽  
Maya Frankfurt ◽  
Victoria Luine
Keyword(s):  
Bisphenol A ◽  
Adult Male ◽  
Dendritic Spine ◽  
Male Rats ◽  
Spine Density ◽  
Dendritic Spine Density

scholarly journals The Effects of Bisphenol A Exposure at Different Developmental Time Points in an Androgen-Sensitive Neuromuscular System in Male Rats

Endocrinology ◽  
2016 ◽  
Vol 157 (8) ◽  
pp. 2972-2977 ◽  
Author(s):  
Bryan A. Jones ◽  
Lydia S. Wagner ◽  
Neil V. Watson
Keyword(s):  
Bisphenol A ◽  
Motor Neurons ◽  
Developmental Time ◽  
Male Rats ◽  
Lumbar Spinal Cord ◽  
Sexually Dimorphic ◽  
Adult Rats ◽  
Soma Size ◽  
Neural Survival ◽  
Lumbar Spinal

The industrial plasticizer bisphenol A (BPA) is a ubiquitous endocrine disruptor to which the general human population is routinely exposed. Although BPA is well known as an estrogenic mimic, there have been some suggestions that this compound may also alter activity at the androgen receptor. To determine whether BPA does have antiandrogenic properties, we evaluated BPA effects in the spinal nucleus of the bulbocavernosus and dorsolateral nucleus, sexually dimorphic groups of motor neurons in the lumbar spinal cord that are critically dependent on androgens for survival and maintenance, as well as the monomorphic retrodorsolateral nucleus. In experiment 1, we administered varying concentrations of BPA to juvenile rats pre- and postnatally and examined both the number and size of motor neurons in adulthood. In experiment 2, different doses of BPA were given to adult rats for 28 days, after which the soma size of motor neurons were measured. Although no effect of BPA on neural survival or soma size was noted after perinatal BPA exposure, BPA exposure did result in a decrease in soma size in all motor neuron pools after chronic exposure in adulthood. These findings are discussed with regard to putative antiandrogenic effects of BPA; we argue that BPA is not antiandrogenic but is acting through nonandrogen receptor-dependent mechanisms.

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