Baked corn (Zea mays L.) and cooked common bean (Phaseolus vulgaris L.) chips improved enzymatic biomarkers and alleviated inflammation during chronic colitis in vivo (P06-063-19)

Abstract Objectives The aim of this research was to evaluate the anti-inflammatory properties of an oven-baked nixtamalized corn (Zea mays L.)/cooked common bean (Phaseolus vulgaris L.) chip in a model of chronic colitis in vivo. The hypothesis was that the chip consumption would prevent colon barrier disruption and improve enzymatic biomarkers by the modulation of infiltration and adhesion of inflammatory cells. Methods A 70% corn and 30% bean chip (7030C) was evaluated, using dextran sodium sulfate (DSS, 2% v/v) as chemical inductor of colitis. After 1-week of acclimatization, 36 CD-1 male mice (6–8 weeks age) were randomly divided into 5 groups: G1 (negative control, fed with basal diet, BD, and water); G2 (positive control, BD + DSS), G3 (100 g 7030C/kg body weight, BW/day + BD), G4 (200 g 7030C/kg BW + BD) and G5 (300 g 7030C/kg BW + BS). The G2-G5 groups were administered DSS every other week, during 5-weeks.After the mice were euthanized, BW and disease activity index (DAI) were recorded. Liver, colon, and spleen were collected, weighed and analyzed for histology. Colonic myeloperoxidase (MPO)/fecal b-glucuronidase (GLUC) activities were also quantified, as well as fecal/cecal metabolites. The colonic mRNA expression of inflammation-associated genes was conducted using a gene inflammation profiler array. Results DSS increased DAI up to 2 units, BW loss was 10–17%, and induced colon shortening 10–15%. Compared to G2, G4 exhibited significantly (p < 0.05) lower DAI (0.75 ± 0.01), spleen relative weight (0.003 ± 0.0001) and colon weight/length ratio (0.045 ± 0.008). The histological analysis showed that the chip consumption prevented colonic barrier damage. G4 displayed the lowest MPO and GLUC among all DSS-induced groups (0.004 ± 0.0004 mU/mg colon; 0.44 ± 0.01 mmol/min/g feces, respectively), and the lowest seric content of MCP-1 protein. Amid all the quantified metabolites, the chip consumption significantly reduced the fecal/cecal content of acetic acid, while butyric and propionic increased at the end of the study. Inflammation gene expression was modulated by the chip consumption. Conclusions Our results suggest that the consumption of this chip might alleviate chronic colitis symptoms because of a protective effect in the gut barrier function and the modulation of infiltration of inflammatory cells. Funding Sources The funding received by CONACyT, CONCyTEQ-Mexico and NIFA-USDA-HATCH are appreciated.

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Baked Corn (Zea mays L.) and Cooked Common Bean (Phaseolus vulgaris L.) Snack Consumption Reduced Inflammation and Upregulated NRF2 and SOD2 in Chronic Colitis In Vivo

Current Developments in Nutrition ◽

10.1093/cdn/nzab044_026 ◽

2021 ◽

Vol 5 (Supplement_2) ◽

pp. 595-595

Author(s):

Ivan Luzardo ◽

Rocio Campos-Vega ◽

Liceth Cuellar-Nuñez ◽

Elvira Gonzalez de Mejia ◽

Guadalupe Loarca-Pina

Keyword(s):

Oxidative Stress ◽

Fatty Acids ◽

Zea Mays ◽

Phaseolus Vulgaris ◽

Zea Mays L ◽

Short Chain Fatty Acids ◽

Phaseolus Vulgaris L ◽

Chronic Colitis ◽

Snack Consumption

Abstract Objectives This research aimed to evaluate the effect of the consumption of a baked corn and bean snack in the intestinal oxidative stress and energetic metabolism in chronic colitis in vivo. Methods The polyphenolic composition of baked 70% nixtamalized corn (Zea mays L.) and 30% cooked common bean (Phaseolus vulgaris L.) snack (70/30) was characterized by UHPLC-QTOF/MS. Dextran sodium sulfate (DSS, 2% v/v) was used as chemical inductor of colitis in 45 CD-1/ICR male mice (6–8 weeks age) randomly distributed in 5 groups (treatment 5 weeks): G1 (basal diet: BD + water), G2 (BD), G3 (20 g 70/30/kg body weight: BW/day + BS), G4 (40 g 70/30/kg BW/day + BS), and G5 (60 g 70/30/kg BW/day + BS). G2-G5 groups were administered DSS every other week, during 5-weeks. BW and disease activity indexes (DAI) were measured weekly. Liver and colon histopathological and immunohistochemical (TGF-b and Ki-67) analysis was performed. Serum antioxidant capacity, pro-inflammatory cytokines, and fecal composition (polyphenols and short-chain fatty acids) were quantified. The colonic mRNA expression of genes associated with oxidative stress and energy metabolism was performed using a mice gene array. Results p-Coumaric and ferulic acids were the main identified phenolics (72–103 mg/g snack, respectively). G3 and G4 exhibited the highest BW (+2.94%) and lowest DAI scores (0.5–1.5) (p < 0.05) among the DSS-induced groups, compared to G2. G4 showed preservation of colon architecture from 70/30-administered groups (histological score: 4.30 ± 0.13), while G3 and G4 exhibited the highest seric antioxidant capacity values (130–147 mg equivalents Trolox/mL). Snack-added groups displayed the lowest IL-6 and TNF-a values (598–657 pg/mL and 82–277 pg/mL, respectively) (p < 0.05) and the highest amount of fecal polyphenols and short-chain fatty acids (21–25 mM). G1 and G4 were clustered in the same groups from the gene analysis (p < 0.05), being the adiponectin receptor 1 (ADIPOR1) (-0.17 fold), erythroid-derived 2-like 2 (NRF2) (0.32–0.34 fold), and superoxide dismutase (SOD2) (0.58–1.25 fold) the main regulated genes, compared to G2. Conclusions Results suggested that 70/30 baked corn and bean snack consumption protects the colon from inflammatory symptoms, decreasing oxidative stress in vivo. Funding Sources The funding received by CONACyT is appreciated.

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