Detection of Pneumococcal DNA in Blood by Polymerase Chain Reaction for Diagnosing Pneumococcal Pneumonia in Young Children From Low- and Middle-Income Countries

PURPOSE Low- and middle-income countries have high incidences of cervical cancer linked to human papillomavirus (HPV), and without resources for cancer screenings these countries bear 85% of all cervical cancer cases. To address some of these needs, brigade-style screening combined with sensitive polymerase chain reaction–based HPV testing to detect common high-risk HPV genotypes may be necessary. METHODS We deployed an inexpensive DNA extraction technique and a real-time polymerase chain reaction–based HPV genotyping assay, as well as Papanicolaou testing, in a factory in San Pedro Sula, Honduras, where 1,732 women were screened for cervical cancer. RESULTS We found that 28% of participants were positive for high-risk HPV, with 26% of HPV-positive participants having more than one HPV infection. Moreover, the most common HPV genotypes detected were different than those routinely found in the United States. CONCLUSION This work demonstrates a deployable protocol for HPV screening in low- and middle-income countries with limited resources to perform cytopathology assessment of Pap smears.

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Evaluation of Pneumococcal Load in Blood by Polymerase Chain Reaction for the Diagnosis of Pneumococcal Pneumonia in Young Children in the PERCH Study

Clinical Infectious Diseases ◽

10.1093/cid/cix149 ◽

2017 ◽

Vol 64 (suppl_3) ◽

pp. S357-S367 ◽

Cited By ~ 8

Author(s):

Maria Deloria Knoll ◽

Susan C. Morpeth ◽

J. Anthony G. Scott ◽

Nora L. Watson ◽

Daniel E. Park ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Young Children ◽

Pneumococcal Pneumonia ◽

Chain Reaction ◽

Polymerase Chain

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Comparison ofEscherichia coliuropathogenic genes (kps,uspandireA) and enteroaggregative genes (aggRandaap) via multiplex polymerase chain reaction from suprapubic urine specimens of young children with fever

Scandinavian Journal of Urology and Nephrology ◽

10.1080/00365590802299338 ◽

2009 ◽

Vol 43 (1) ◽

pp. 51-57 ◽

Cited By ~ 11

Author(s):

Hae-Kyung Park ◽

Yun-Jae Jung ◽

Hee-Chung Chae ◽

Yoo-Jung Shin ◽

So-Youn Woo ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Young Children ◽

Multiplex Polymerase Chain Reaction ◽

Chain Reaction ◽

Polymerase Chain ◽

Urine Specimens

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RAPID AND SPESIFIC DETECTION OF MYCOBACTERIUM TUBERCULOSIS USING POLYMERASE CHAIN REACTION

Journal Of Vocational Health Studies ◽

10.20473/jvhs.v3.i2.2019.83-88 ◽

2019 ◽

Vol 3 (2) ◽

pp. 83

Author(s):

Anita Kurniati ◽

Desak Nyoman Surya Suameitra Dewi ◽

Ni Nyoman Purwani

Keyword(s):

Polymerase Chain Reaction ◽

Mycobacterium Tuberculosis ◽

Clinical Specimen ◽

Laboratory Diagnosis ◽

Nucleic Acid Amplification ◽

Valuable Insight ◽

Chain Reaction ◽

Severe Condition ◽

Middle Income ◽

Polymerase Chain

Background: Tuberculosis (TB) is one of the major causes of health burden worldwide, especially in lower middle-income countries. TB is caused by Mycobacterium tuberculosis (MTB) and characterized by severe condition incuding coughing and fever. Purpose: To review the current methods for detection of TB using Polymerase Chain Reaction (PCR). Review: several studies have been done to give valuable insight into TB transmission, diagnosis, and treatment, however research is constantly needed to decrease the incidence of eradicate TB. This infectious disease still give big health problem in all over the world by being second in causing high mortality rates after HIV/AIDS. A specific, sensitive, rapid and cheap method for TB and other mycobacteria diagnosis in clinical specimen is a desperate needed in the laboratory diagnosis and hence management of tuberculosis. PCR as one of nucleic acid amplification assays have revolutionized MTB detection. Since it was first invented in fifteen years ago, it’s been through many developments. Conclusion: PCR is one of the most specific and sensitive method currently available for TB diagnosis that can also detect in in all types of specimens obtained from TB patients.

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