An Automated Determination of Alkaline Phosphatase Utilizing p-Nitrophenol Phosphate

1964 ◽  
Vol 10 (12) ◽  
pp. 1112-1116 ◽  
Author(s):  
Rex E Sterling ◽  
Alan A Wilcox ◽  
Arnold G Ware ◽  
Mary K Umehara
Keyword(s):  
Alkaline Phosphatase ◽  
Production Rate ◽  
Serum Alkaline Phosphatase ◽  
Automated Determination

Abstract A technic is described for the automated determination of serum alkaline phosphatase. This method permits a production rate of 60 determinations per hour on 0.2 ml. of serum per determination. Calculations are simplified since no serum blank is required.

An Automated p-Nitrophenylphosphate Serum Alkaline Phosphatase Procedure for the AutoAnalyzer

1965 ◽  
Vol 11 (9) ◽  
pp. 876-888 ◽  
Author(s):  
Stanley Morgenstern ◽  
Gerald Kessler ◽  
Joseph Auerbach ◽  
Richard V Flor ◽  
Bernard Klein
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Reaction Rates ◽  
Small Sample ◽  
Automated System ◽  
Enzyme Action ◽  
Linear Reaction ◽  
Simplified Procedure ◽  
Automated Determination

Abstract A new and simplified procedure for the automated determination of serum alkaline phosphatase uses the AutoAnalyzer. The substrate p-nitrophenylphosphate in 2-amino-2-methyl-1-propanol offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. The procedure also permits use of small sample volumes, provides linear reaction rates, and has a simple manifold design. Correlations are presented between the manual procedure and the automated system.

An Automated p-Nitrophenylphosphate Serum Alkaline Phosphatase Procedure for the "Robot Chemist"

1965 ◽  
Vol 11 (9) ◽  
pp. 889-897 ◽  
Author(s):  
Stanley Morgenstern ◽  
Gerald Kessler ◽  
Joseph Auerbach ◽  
Richard V Flor ◽  
Bernard Klein
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Reaction Rates ◽  
Small Sample ◽  
Enzyme Action ◽  
Nitrophenyl Phosphate ◽  
Linear Reaction ◽  
Accurate Procedure ◽  
Automated Determination

Abstract A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenyl-phosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample volumes required and linear reaction rates. Correlations are presented between the manual procedure and those used with both the Technicon Auto-Analyzer and the Robot Chemist.

Automated Determination of Serum Alkaline Phosphatase Using a Modified Bodansky Technic

1964 ◽  
Vol 10 (1) ◽  
pp. 75-82 ◽  
Author(s):  
H Keay ◽  
J A Trew
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Inorganic Phosphate ◽  
Serum Alkaline Phosphatase ◽  
Automated Determination

Abstract An automated procedure for the measurement of alkaline phosphatase activity by a modification of the Bodansky method is described. It has been possible to adapt the Fiske-SubbaRow method for phosphate so that alkaline phosphatase is determined by a second run, immediately following the determination of inorganic phosphate. Studies of the effect of time, pH, and concentration of barbital on the enzyme activity are discussed, and the advantages of the method are listed.

Automated Alkaline Phosphatase Determination

1967 ◽  
Vol 13 (4) ◽  
pp. 290-298 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman
Keyword(s):  
Alkaline Phosphatase ◽  
Excellent Agreement ◽  
Enzyme Activities ◽  
Present Method ◽  
Serum Alkaline Phosphatase ◽  
Control Analysis

Abstract An automated procedure for the determination of serum alkaline phosphatase uses phenolphthalein monophosphate as the substrate. Important assets of this substrate are: (1) A chromogenic product is enzymically produced and can be measured directly; (2) Bilirubin does not interfere, thus eliminating the need for a control analysis. Excellent agreement is obtained when the enzyme activities determined by the present method and by the automated p-nitrophenylphosphate procedure are compared.

An Automated Alkaline Phosphatase Assay with Phenolphthalein Monophosphate as Substrate

1967 ◽  
Vol 13 (4) ◽  
pp. 281-289 ◽  
Author(s):  
Kirsten Hviid
Keyword(s):  
Alkaline Phosphatase ◽  
Normal Values ◽  
Serum Alkaline Phosphatase ◽  
Colorimetric Determination ◽  
Alkaline Phosphatase Assay ◽  
Phosphatase Assay ◽  
Hydrolysis Of

Abstract The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual procedure, and data relating to sample interaction, precision, blank values, and normal values are presented.

BIOASSAY OF PARATHYROID HORMONE IN RATS BY DETERMINATION OF PLASMA CALCIUM, URINARY 32P EXCRETION AND SERUM ALKALINE PHOSPHATASE

1966 ◽  
Vol 35 (3) ◽  
pp. 229-238 ◽  
Author(s):  
R. J. TREACHER
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Serum Calcium ◽  
Calcium Concentration ◽  
Serum Alkaline Phosphatase ◽  
Plasma Calcium ◽  
Significant Alteration ◽  
Good Precision ◽  
Serum Calcium Concentration

SUMMARY Methods for assay of parathyroid hormone based on an increase in serum calcium concentration, urinary 32P excretion and serum alkaline phosphatase elevation in parathyroidectomized rats have been compared and modifications introduced to improve sensitivity, precision, speed and ease of manipulation. Both the serum calcium and urinary 32P assay gave good precision (mean λ = 0·23 and 0·29, respectively) but by the serum calcium method less than 10 USP units of parathyroid hormone could not be detected, whereas the phosphaturic assay detects as little as 0·5 USP unit. Both assays are simple to perform and each requires only 2 days to complete. They can be combined in a single design using the same animals. Assays based on serum alkaline phosphatase levels in parathyroidectomized rats were not successful since it was impossible to produce a significant alteration in serum alkaline phosphatase by the administration of parathyroid hormone.

scholarly journals Diagnosis for severe Babesia gibsoni Infection in Dogs by determination of serum alkaline phosphatase

1994 ◽  
Vol 47 (6) ◽  
pp. 415-418 ◽  
Author(s):  
Kazuhiko NAMIKAWA ◽  
Toru ISHIBASHI ◽  
Fujiko SUNAGA ◽  
Yasunori KANNO
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Babesia Gibsoni

Rapid Method for the Quantitative Determination of Serum Alkaline Phosphatase

1960 ◽  
Vol 6 (3) ◽  
pp. 269-275 ◽  
Author(s):  
Bernard klein ◽  
Prunella A Read ◽  
Arthur L Babson
Keyword(s):  
Alkaline Phosphatase ◽  
Quantitative Determination ◽  
Rapid Method ◽  
Quantitative Measurement ◽  
Serum Alkaline Phosphatase ◽  
Photometric Measurement

Abstract A new procedure is described for the simple, rapid, quantitative measurement of serum alkaline phosphatase. The procedure is based on the direct photometric measurement of phenolphthalein released from sodium phenolphthalein phosphate. The substrate is available in a convenient, stabilized form as a tablet containing buffer and substrate in optimum amounts for a single analysis. As many as 30 analyses can be performed in an hour.

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