Correlation of Serum and Urine Enzyme Activity in Patients with Acute Myocardial Infarction

1967 ◽  
Vol 13 (5) ◽  
pp. 359-370 ◽  
Author(s):  
Albert A Dietz ◽  
LaVerne K Hodges ◽  
Donald T Foxworthy
Keyword(s):  
Myocardial Infarction ◽  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Anticoagulation Therapy ◽  
Control Group ◽  
Average Value ◽  
Essentially Normal ◽  
Transmural Infarction ◽  
Urinary Acid

Abstract A correlation was made between the urinary excretion of GOT, GPT, LDH, HBD, and acid and alkaline phosphatase and their serum activity in 18 suspected cases of myocardial infarction. The diagnoses were made by a cardiologist without knowledge of the enzyme assays. The study included 2 cases of nontransmural infarction; 5 cases that had not sustained an infarction served as controls. Elevations of serum GOT, LDH, and HBD were found in 10 of 11 cases of transmural infarction, GPT and acid phosphatase in 5, and alkaline phosphatase in 7. Elevations in serum acid phosphatase were also found in the control group, and perhaps were related to the anticoagulation therapy. Urinary GOT and GPT activity was variable. Urinary LDH and HBD were elevated in 6 of the transmural infarction cases, usually when serum LDH and HBD activity was decreasing. Urinary acid phosphatase activity was essentially normal; but urinary alkaline phosphatase increased in 10 of the transmural infarction cases, and the average value remained high for more than 5 weeks. The only index that was elevated in all cases of acute transmural infarction was L-phenylalanine inhibition of urinary alkaline phosphatase activity.

scholarly journals Indicators of bone metabolism in patients of psychiatric clinic on the background of dimephosphone intake

2016 ◽  
Vol 97 (5) ◽  
pp. 669-673
Author(s):  
R Yu Il’ina ◽  
O O Pasynkova ◽  
L R Mukhamedzhanova ◽  
L E Ziganshina
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Mental Disorders ◽  
Bone Metabolism ◽  
Phosphatase Activity ◽  
Serum Calcium ◽  
Alkaline Phosphatase Activity ◽  
Psychiatric Patients ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase

Aim. To study the indicators of bone metabolism in patients of psychiatric clinics on the background of dimephosphone oral intake.Methods. Research was conducted at the Republican Clinical Psychiatric Hospital named after V.M. Bekhterev (Kazan). 64 patients (36 men and 28 women) aged 45 to 68 years (mean age 56.8±6.9 years) with various forms of mental disorders taking psychotropic drugs for the period of 3 years and over were examined. Resorption markers were determined - D-pyridinoline in urine, serum tartrate-resistant acid phosphatase, alkaline phosphatase, and the content of total and ionized serum calcium. All patients were divided into two groups: those who received dimephosphone in the combined systemic therapy and those who did not. The average duration of the dimephosphone administration was 22±4.2 days, the patients undergone on average 2.6±1.44 courses of treatment during 6 months.Results. The activity of tartrate-resistant acid phosphatase in psychiatric patients was higher compared with those of the control group. The maximum value was registered in patients who were not taking dimephosphone. The alkaline phosphatase activity was the highest in mentally healthy patients of the control group. In psychiatric patients treated with dimephosphone, alkaline phosphatase activity was 20% lower compared with the control group. The minimum values of alkaline phosphatase were registered in patients with mental disorders not taking dimephosphone. When studying the concentration of total and ionized serum calcium there were no significant differences between patients of comparison group and psychiatric patients.Conclusion. Shift in processes of bone remodeling toward its resorption indicates a high risk of pathological fractures in psychiatric patients and the need for osteoporosis prevention; identified changes in biochemical markers of osteoporosis on the background of dimephosphone intake allow to recommend it for the treatment of diagnosed osteoporosis in a psychiatric hospital settings.

Acid phosphatase, alkaline phosphatase, and nitrate reductase activity of selected ectomycorrhizal fungi

1989 ◽  
Vol 67 (3) ◽  
pp. 750-753 ◽  
Author(s):  
Iwan Ho
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Nitrate Reductase ◽  
Phosphatase Activity ◽  
Ectomycorrhizal Fungi ◽  
Acid Phosphatase Activity ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Phosphatase Alkaline

Seventeen isolates, encompassing five genera and eight species of ectomycorrhizal fungi, were compared for acid phosphatase, alkaline phosphatase, and nitrate reductase activity. Isolates within species differed in enzyme activity and isozyme patterns by host specificity and site (as exemplified by the genus Suillus). Host and site may have affected phosphatase enzyme activity. Generally, the Douglas-fir associates, which dominate in mesic sites, have higher acid phosphatase activity than pine associates, which mostly occupy xeric sites; however, pine associates from mesic sites also have higher acid phosphatase activity (e.g., S. tomentosus). In four isolates of Amanita muscaria, the effect of site was also apparent. Two of them, which have significantly higher acid phosphatase activity than the others, were isolated from mesic sites. The isozyme pattern of the genus Suillus appeared to be separated by host groups. Other isolates with only one species also differed more or less by host groups. They shared at least one band within host groups, except for the two isolates of Paxillus involutus from different hosts. The P. involutus S-403 isolated from an orchard showed much higher nitrate reductase activity than all other isolates. No apparent differences in nitrate reductase activity were found between the other isolates.

scholarly journals The effect of different copper doses and organic fertilisation on soil’s enzymatic activity

2020 ◽  
Vol 66 (No. 2) ◽  
pp. 93-98
Author(s):  
Beata Kuziemska ◽  
Andrzej Wysokiński ◽  
Joanna Trębicka
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Enzymatic Activity ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Organic Materials ◽  
Chicken Manure ◽  
Test Plant ◽  
Orchard Grass ◽  
Organic Fertilisation

A three-year pot experiment carried out in the vegetation hall in 2014–2016 included studying the enzymatic activity of soil, into which various amounts of copper: (100, 200 and 300 mg Cu/kg soil) and organic materials (cattle manure, chicken manure, post-mushroom substrate) were introduced, used separately, at a soil-introduction dose of 2 g C<sub>org</sub>/kg. Copper and organic materials were used once, only in the first year of the study, before sowing test plant orchard grass. In soil collected after the last (fourth) swath of grass in each year of the study, the activity of urease, dehydrogenases, acid, and alkaline phosphatase was determined. Applications of copper to the soil, regardless of its dose, resulted in a decrease in urease, dehydrogenases and alkaline phosphatase and an increase in acid phosphatase activity. The inactivating effect of this metal on the activity of urease, dehydrogenases and alkaline phosphatase increased with the increase of its dose. Organic fertilisation generally increased the enzymatic activity of the analysed soil. In subsequent years of the study, urease and alkaline phosphatase activity decreased, while acid phosphatase activity increased. Dehydrogenase activity did not change significantly in subsequent years of the study.  

Acid and alkaline phosphatases in the lymphomyeloid tissues of elasmobranch fish

1978 ◽  
Vol 58 (3) ◽  
pp. 727-730 ◽  
Author(s):  
Ragnar Fänge
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
High Activity ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Alkaline Phosphatases ◽  
Large Numbers ◽  
Alkaline Reaction ◽  
Elasmobranch Fish ◽  
Very High

Activities of phosphomonoesterases were measured at acid and at alkaline reaction (pH 4–5 or 9–65) in homogenates of elasmobranch tissues especially lymphomyeloid structures. The animals were dogfish (Scyliorhinus caniculd) and two species of ray (Raja brachyura, R. naevus). Acid phosphatase activity was high in the epigonal tissue, Leydig's organ, the spleen and the thymus. High activity was also found in the pancreas and the kidney, whereas skeletal and cardiac muscle showed low values. The activity of alkaline phosphatase was very high in the kidney and relatively low in other tissues. Ultrasonification of homogenates from the dogfish resulted in increase of acid phosphatase activity but had little effect on alkaline phosphatase activity. The high activity of acid phosphatase in lymphomyeloid tissue may be due to the presence of large numbers of various types of leucocytes.

scholarly journals HISTOCHEMICAL DEMONSTRATION OF ACID PHOSPHATASE ACTIVITY IN OSTEOCLASTS

1959 ◽  
Vol 7 (1) ◽  
pp. 39-41 ◽  
Author(s):  
M. S. BURSTONE
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Comparative Studies ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
Histochemical Demonstration ◽  
Accurate Localization ◽  
Acid And Alkaline Phosphatase ◽  
Cold Acetone

High acid phosphatase activity was observed in osteoclasts of several species using a reproducible azo-dye technique. High activity of two distinct enzymes, acid and alkaline phosphatase, are associated with osteoclasts and osteoblasts respectivey. Althouth frozen-dried tissues are recommended for definitive studies, the enzyme techniques used give satisfactory results with cold acetone-fixed tissues. The most accurate localization of acid phosphatase in osteoclasts in controlled comparative studies is obtained with double-embedded frozen-dried undecalcified tissues in conjunction with naphthol AS-phosphates.

scholarly journals Cytotoxicity and osteogenic potential of experimental medication with calcium hydroxide and activated charcoal

2021 ◽  
Vol 10 (5) ◽  
pp. e26010514671
Author(s):  
Gabriela Sumie Yaguinum Gonçalves ◽  
Danielle Gregorio ◽  
Isabelly Ribeiro Custódio ◽  
Luciana Prado Maia ◽  
Bruno Piazza ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Calcium Hydroxide ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Activated Charcoal ◽  
Osteogenic Potential ◽  
Control Group ◽  
L929 Cells ◽  
Tukey Test

Objectives: The aim of this study was to evaluate the cytotoxicity and osteogenic potential of experimental medications containing Calcium Hydroxide (CH) and Activated charcoal (AC). Methodology: Osteoblastic (MC3T3) and fibroblastic (L929) cells were cultivated in 96 well plates (1 x 104 cells/well) and, after 24 h, treated with extracts, according to experimental groups [(Experimental groups: C - Control; CH - CH paste; CH+10%AC - experimental paste 1 (CH paste + 10% AC); CH+5%AC - experimental paste 2 (CH paste + 5% AC)]. Cytotoxicity and potential osteogenic was performed by MTT and alkaline phosphatase activity, respectively, after 1, 3 and 7 days. Results: For intergroups comparisons, ANOVA 2 factors were used, followed by Tukey test (p < 0.05). There was no difference among the pastes for cytotoxicity in both cells (p > 0.05). For osteogenic potential, it was found that all experimental groups stimulated mineralization in relation to control group, except for experimental paste 2 at 7 days. Conclusion: The addition of AC to the CH paste does not modify toxicity and osteogenic properties. Add 10% of AC appears to be more effective than 5%.

THE DETERMINATION OF URINARY ACID PHOSPHATASES

1952 ◽  
Vol 30 (1) ◽  
pp. 1-9
Author(s):  
G. E. Delory ◽  
Merle Hetherington
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Convenient Method ◽  
Acid Phosphatase Activity ◽  
Acid Phosphatases ◽  
Urinary Acid

The effect of dilution on the apparent acid phosphatase activity of undialyzed and dialyzed urine has been studied. In the former case, the apparent activity increases with dilution but this anomaly is removed by a preliminary dialysis. A convenient method for the determination of acid phosphatase based on this observation is described.

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