A New Specific Assay for Urinary Creatinine

1969 ◽  
Vol 15 (9) ◽  
pp. 879-883 ◽  
Author(s):  
Surendra N Sinha ◽  
Elemer R Gabrieli
Keyword(s):  
Simultaneous Determination ◽  
Phosphate Buffer ◽  
Hippuric Acid ◽  
Gel Filtration ◽  
Urinary Creatinine ◽  
Specific Assay ◽  
Other Substances ◽  
Subsequent Measurement ◽  
Standard Deviations

Abstract A new specific assay for urinary creatinine is described. The procedure is based on the separation of creatinine from other urinary components by Sephadex gel filtration, and subsequent measurement of the compound at 235 nm against a phosphate buffer blank. No interference by other substances has been encountered. The standard deviations in a series of assays using standard solutions are less than 2%, and the recovery of added known amounts of creatinine from urine is excellent. The method has been found satisfactory for simultaneous determination of creatinine and hippuric acid in urine.

Collaborative Study of the Quantitative Determination of Fusel Oil and Ethyl Acetate by Gas-Liquid Chromatography

1968 ◽  
Vol 51 (4) ◽  
pp. 915-921
Author(s):  
Richard L Brunelle
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Ethyl Acetate ◽  
Simultaneous Determination ◽  
Collaborative Study ◽  
Gas Liquid Chromatography ◽  
Fusel Oil ◽  
Preliminary Work ◽  
Standard Deviations

Abstract Thirteen chemists representing twelve laboratories participated in a collaborative study for the quantitative determination of ethyl acetate and fusel oil by GLC. The results on four samples containing various amounts of ethyl acetate and fusel oil were compared with the official AOAC procedures. For all samples, the standard deviations were lower with GLC. Collaborators preferred the GLC procedure because the simultaneous determination of ethyl acetate and n-propyl, iso-butyl, and isoamyl alcohols is possible. Results from preliminary work performed on the quantitative determination of acetaldehyde by GLC were encouraging. It is recommended that the proposed GLC procedure for the quantitative determination of ethyl acetate and fusel oil be adopted as official, first action, and that work be continvied on the determination of acetaldehyde by GLC.

scholarly journals Multiwavelength Spectral Correction for the Simultaneous Determination of Cu(II), Fe(II), and Zn(II) with 1-(5-Bromo-2-Pyridylazo)-2-Naphthol-6-Sulfonic Acid

2001 ◽  
Vol 84 (1) ◽  
pp. 532-536 ◽  
Author(s):  
Hong-Wen Gao
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Sulfonic Acid ◽  
Free Ligand ◽  
Spectral Correction ◽  
Relative Standard ◽  
Zn And Cu In ◽  
Matrix Formula ◽  
Standard Deviations

Abstract A new analytical method is described for the simultaneous determination of various components by multiwavelength spectrophotometry. Because of the influence of the free ligand and various complexes on spectrophotometric absorption, the spectral correction principle was used to establish the calculation matrix formula. The 3 sensitive reactions of Cu(II), Fe(II), and Zn(II), with 1-(5-bromo-2-pyridylazo)-2-naphthol-6-sulfonic acid were studied at pH 10.5. In analyses of fortified samples, the recoveries of Fe, Zn, and Cu were between 93.0 and 103%, 87.0 and 108%, and 92.5 and 108%, respectively; the relative standard deviations for 5 determinations of Fe, Zn, and Cu in unfortified ore were 3.6, 5.8, and 4.5%, respectively.

scholarly journals Liquid Chromatographic Determination of Fumonisins B1, B2, and B3 in Corn: AOAC–IUPAC Collaborative Study

1996 ◽  
Vol 79 (3) ◽  
pp. 688-696 ◽  
Author(s):  
Eric W Sydenham ◽  
Gordon S Shephard ◽  
Pieter G Thiel ◽  
Sonja Stockenström ◽  
Petra W Snijman ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
Relative Standard ◽  
Valid Data ◽  
Liquid Chromatographic Determination ◽  
Standard Deviations ◽  
The Individual ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method for simultaneous determination of fumonisins B1 (FB1), B2 (FB2), and B3 (FB3) in corn was subjected to a collaborative study involving 12 participants from 10 countries, in which the accuracy and reproducibility characteristics of the method were established. Mean analyte recoveries from corn ranged from 81.1 to 84.2% for FB1 (at a spiking range of 500 to 8000 ng/g), from 75.9 to 81.9% for FB2 (at a spiking range of 200 to 3200 ng/g), and from 75.8 to 86.8% for FB3 (at a spiking range of 100 to 1600 ng/g). The valid data were statistically evaluated after exclusion of outliers. Relative standard deviations for within-laboratory repeatability ranged from 5.8 to 13.2% for FB1, from 7.2 to 17.5% for FB2, and from 8.0 to 17.2% for FB3. Relative standard deviations for between-laboratory reproducibility varied from 13.9 to 22.2% for FB1, from 15.8 to 26.7% for FB2, and from 19.5 to 24.9% for FB3. HORRAT ratios, calculated for the individual toxin analogues, ranged from 0.75 to 1.73. The LC method for determination of fumonisins B1, B2, and B3 in corn (at concentrations of 800–12800 ng total fumonisins/g) has been adopted by AOAC INTERNATIONAL.

scholarly journals Development and Validation of HPLC-DAD Method for Simultaneous Determination of Seven Food Additives and Caffeine in Powdered Drinks

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1119
Author(s):  
Imanulkhan Imanulkhan ◽  
Widiastuti Setyaningsih ◽  
Abdul Rohman ◽  
Miguel Palma
Keyword(s):  
Simultaneous Determination ◽  
Phosphate Buffer ◽  
High Performance ◽  
Desirability Function ◽  
Food Additives ◽  
Complete Separation ◽  
Sodium Saccharin ◽  
Array Detection ◽  
Development And Validation

The usage of food additives must respect the general legislation in force in the country and requires a reliable analytical method for surveillance. This research aimed to develop a high-performance liquid chromatography with diode array detection (HPLC-DAD) method for the simultaneous determination of seven food additives and caffeine in powdered drinks. Three factors likely to affect the chromatographic separation, namely, mobile phase composition at the beginning (x1, 0–10% of the amount of methanol in the phosphate buffer) and the end (x2, 60–100% of the amount of methanol in the phosphate buffer) of the gradient program and pH (x3, 3–7), were evaluated with the aid of a Box–Behnken Design (BBD). Subsequently, multi-response optimizations for chromatographic resolutions (Rs) and analysis time were performed using the response surface methodology (RSM) in conjunction with the desirability function (DF). Complete separation (Rs > 1.5) of seven food additives and caffeine was achieved in less than 16 min by applying 8.5% methanol in the phosphate buffer at the beginning and 90% at the end of the gradient program, in pH 6.7. The developed method was validated with low limits of detection (ranging from 1.16 mg kg−1 (sodium saccharin) to 3.00 mg kg−1 (acesulfame potassium)), low limits of quantification (ranging from 3.86 mg kg−1 (sodium saccharin) to 10.02 mg kg−1 (acesulfame potassium)), high precision (CV < 4%), and high accuracy (recoveries from 95 to 101% at 80, 100, and 120% of the target concentration). The method was successfully used to assess the seven food additives and caffeine in commercially available powdered drinks.

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