Nitrile-Terminated Hydrocarbons as Stationary Phases for High-Performance Liquid Chromatography of Steroid Hormones

1973 ◽  
Vol 19 (11) ◽  
pp. 1293-1295 ◽  
Author(s):  
Francis A Fitzpatrick
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Steroid Hormones ◽  
High Performance ◽  
Chromatographic Method ◽  
Stationary Phases ◽  
High Performance Liquid Chromatographic ◽  
Unsaturated Ketone ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A high-performance liquid chromatographic method for separating steroid hormones by using nitrile-terminated hydrocarbons as the stationary phase is described. Particular selectivity toward the separation of corticosteroids with an α, β unsaturated ketone group is noted, with five steroids more polar than cortisol being completely resolved. The system described is also applicable to estrogen separations.

Lactate dehydrogenase inhibitors in NADH preparations.

1977 ◽  
Vol 23 (9) ◽  
pp. 1581-1584 ◽  
Author(s):  
S A Margolis ◽  
B F Howell ◽  
R Schaffer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Lactate Dehydrogenase ◽  
High Performance ◽  
Chromatographic Method ◽  
Deae Cellulose ◽  
High Performance Liquid Chromatographic ◽  
Clinical Assay ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract The presence of a new lactate dehydrogenase inhibitor on the trailing edge of the NADH peak from chromatography on diethylaminoethyl-celluose [Loshon et al., Clin. Chem., this issue] was verified. It was resolved from the NADH by high-performance liquid chromatography on muBondapak C18. When the new inhibitor was present in a reaction mixture to the extent that, of the initial 260-nm absorbance, about 5% was contributed by the inhibitor, the rate of NADH oxidation by lactate dehydrogenase decreased by 65%. The inhibitor absorbs at 260 and 340 nm, and is different from the Strandjord-Clayson inhibitor [J. Lab. Clin. Med. 67, 144 (1966)] by both types of chromatography. Because this new inhibitor has ultraviolet properties similar to those of NADH and chromatographs with the NADH on DEAE-cellulose, the high-performance liquid chromatographic method must be used to ensure its absence in preparations of NADH used for clinical assay.

Liquid Chromatographic Method for Quantifying Atrazine Extracted by Supercritical Extraction

2008 ◽  
Vol 273-276 ◽  
pp. 802-807
Author(s):  
Teresa Castelo-Grande ◽  
Paulo A. Augusto ◽  
Domingos Barbosa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Analytical Method ◽  
Contaminated Soils ◽  
High Performance ◽  
Chromatographic Method ◽  
Supercritical Extraction ◽  
Good Linearity ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

An analytical method, using High Performance Liquid Chromatography, was developed for quantification of atrazine in extracts obtained by supercritical extraction of contaminated soils. The method shows good linearity, for concentrations ranging from 0.1 mg/L to 200 mg/L, and reproducibility, giving deviations lower than 2%. The recovery of atrazine by SCE was in the range of 96 to 98%.

scholarly journals Simultaneous determination of hydroxymethylbilane synthase and uroporphyrinogen III synthase in erythrocytes by high-performance liquid chromatography

1983 ◽  
Vol 213 (1) ◽  
pp. 85-88 ◽  
Author(s):  
D J Wright ◽  
C K Lim
Keyword(s):  
High Performance Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Normal Ranges ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Accurate Quantification

A high-performance-liquid-chromatographic method is developed for the simultaneous determination of hydroxymethylbilane synthase and uroporphyrinogen III synthase activity in erythrocytes. Effective separation of uroporphyrin I and III isomers allows the accurate quantification of individual isomers and the total uroporphyrin concentration. Total uroporphyrin production is used to calculate hydroxymethylbilane synthase activity, and the amount of uroporphyrin III formed represents the activity of uroporphyrinogen III synthase. Normal ranges are established for the two enzymes.

scholarly journals High-performance liquid chromatography of phosphatidylcholine and sphingomyelin with detection in the region of 200 nm

1976 ◽  
Vol 155 (1) ◽  
pp. 55-60 ◽  
Author(s):  
F B Jungalwala ◽  
J E Evans ◽  
R H McCluer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Animal Tissues ◽  
Degree Of Unsaturation ◽  
Liquid Chromatographic Method ◽  
Absorbing Material ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

A sensitive method for the separation of phosphatidylcholine and sphingomyelin by high-performance liquid chromatographic analysis is described. The elution of the phospholipids from a microparticulate (10 mum) silica-gel chromatographic column was monitored with an ultraviolet spectromonitor at 203 nm. Acetonitrile/methanol/water (65:21:14, by vol.) was used as the solvent. It was shown by using synthetic phosphatidylcholines of knowm fatty acid composition and of varying degree of unsaturation that the absorption at 203 nm was primarily due to the isolated double bonds and the response measured varied with the degree of unsaturation. Approx. 1 nmol of phosphatidylcholine, containing at least one double bond per molecule, can be detected. The amounts of phosphatidylcholine and sphingomyelin could be determined by high-performance liquid chromatography and ultraviolet absorption if the apparent extinction coefficient of the material analyzed was established. Alternatively, peaks were collected and the phospholipids were determined by the analysis of phosphorus. The analysis of phosphatidylcholine and sphingomyelin present in the lipid extracts from animal tissues, blood and amniotic fluids were made without interference from other phospholipids or ultraviolet-absorbing material. The method described here is complementary to the high-performance liquid chromatographic method described previously for the analysis of ethanolamine-containing phosphoglycerides and serine-containing phosphoglycerides [Jungalwala, Turel, Evans and McCluer (1975) Biochem. J. 145, 517-526].

Free 3-methoxy-4-hydroxyphenylglycol determined in plasma by liquid chromatography with coulometric detection.

1989 ◽  
Vol 35 (2) ◽  
pp. 202-205 ◽  
Author(s):  
M Hariharan ◽  
T VanNoord ◽  
O G Cameron ◽  
G C Curtis ◽  
D G Ostrow
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Sodium Bicarbonate Solution ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Coulometric Detection ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract We describe a simple "high-performance" liquid-chromatographic method for determining free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma, with coulometric detection and with 4-methoxy-3-hydroxyphenylglycol (iso-MHPG) as the internal standard. MHPG and iso-MHPG are extracted from plasma into ethyl acetate and the extract is washed with a sodium bicarbonate solution, evaporated, reconstituted, and injected into a 25 x 0.4 cm column packed with 3-micron particles of C18 material. We used a mobile phase of acetate buffer (100 mmol/L, pH 5.0) and methanol (92:8 by vol) and an oxidation voltage of 0.5 V. The detection limit of the assay is 0.1 micrograms/L. The interassay CV for a sample with a mean MHPG concentration of 3.84 micrograms/L was 5.2%. The average absolute recovery for the method was 37%.

Rapid High Performance Liquid Chromatographic Method for Determination of Gelatin-Coated Supplemental Vitamin E in Feeds

1980 ◽  
Vol 63 (5) ◽  
pp. 1154-1157
Author(s):  
Svend Eriksen
Keyword(s):  
High Performance Liquid Chromatography ◽  
Vitamin E ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Isocratic Elution ◽  
Liquid Chromatographic Method ◽  
Supplemental Vitamin ◽  
Liquid Chromatographic

Abstract A rapid method is described for determining supplemental vitamin E in feeds by means of high performance liquid chromatography (HPLC) using isocratic elution with methanol-water. The sample is mixed with water and left on a water bath to dissolve the coating. The released vitamin E is extracted, the solvent is evaporated, and the sample is redissolved in methanol for injection. The method is applicable to gelatin-coated vitamin preparations. Standard deviations for 2 ranges of supplemented vitamin E, 10–50 ppm and 50–200 ppm, are 1.6 and 5.7, respectively.

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