scholarly journals High-performance liquid chromatography of phosphatidylcholine and sphingomyelin with detection in the region of 200 nm

1976 ◽  
Vol 155 (1) ◽  
pp. 55-60 ◽  
Author(s):  
F B Jungalwala ◽  
J E Evans ◽  
R H McCluer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Animal Tissues ◽  
Degree Of Unsaturation ◽  
Liquid Chromatographic Method ◽  
Absorbing Material ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

A sensitive method for the separation of phosphatidylcholine and sphingomyelin by high-performance liquid chromatographic analysis is described. The elution of the phospholipids from a microparticulate (10 mum) silica-gel chromatographic column was monitored with an ultraviolet spectromonitor at 203 nm. Acetonitrile/methanol/water (65:21:14, by vol.) was used as the solvent. It was shown by using synthetic phosphatidylcholines of knowm fatty acid composition and of varying degree of unsaturation that the absorption at 203 nm was primarily due to the isolated double bonds and the response measured varied with the degree of unsaturation. Approx. 1 nmol of phosphatidylcholine, containing at least one double bond per molecule, can be detected. The amounts of phosphatidylcholine and sphingomyelin could be determined by high-performance liquid chromatography and ultraviolet absorption if the apparent extinction coefficient of the material analyzed was established. Alternatively, peaks were collected and the phospholipids were determined by the analysis of phosphorus. The analysis of phosphatidylcholine and sphingomyelin present in the lipid extracts from animal tissues, blood and amniotic fluids were made without interference from other phospholipids or ultraviolet-absorbing material. The method described here is complementary to the high-performance liquid chromatographic method described previously for the analysis of ethanolamine-containing phosphoglycerides and serine-containing phosphoglycerides [Jungalwala, Turel, Evans and McCluer (1975) Biochem. J. 145, 517-526].

scholarly journals Sensitive analysis of ethanolamine- and serine-containing phosphoglycerides by high-performance liquid chromatography

1975 ◽  
Vol 145 (3) ◽  
pp. 517-526 ◽  
Author(s):  
F B Jungalwala ◽  
R J Turel ◽  
J E Evans ◽  
R H McCluer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
High Performance Liquid Chromatographic ◽  
Amino Groups ◽  
Tissue Samples ◽  
Primary Amino ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

A highly sensitive method for the separation and quantitative measurement of phospholipids containing primary amino groups, such as phosphatidylethanolamine, phosphatidylserine and lysophosphatidylethanolamine, is described. The method involves a simple and quantitative derivative formation of the phospholipids containing amino groups to their u.v.-absorbing biphenylcarbonyl derivatives. These have molar extinction coefficients of about 23,000 at 268nm. The phospholipid derivatives are then separated and non-destructively determined by high-performance liquid chromatography. The amino phospholipids containing vinyl ether bonds (plasmalogens) can be determined separately from the diacyl- and alkylacyl-amino phospholipids. The lower limit of detection by high-performance liquid-chromatographic analysis of the phospholipid derivatives is about 10-13pmol or 0.3-0.4ng of phospholipid P. The quantitative range of derivative formation and analysis by high-performance liquid chromatography of the phospholipids containing amino groups was shown to be 10-500nmol. The method was shown to be applicable to the analysis of phospholipids containing amino groups in tissue samples.

Application of "high-performance" liquid chromatography to the study of sphingolipidoses.

1980 ◽  
Vol 26 (10) ◽  
pp. 1499-1503 ◽  
Author(s):  
M D Ullman ◽  
R E Pyeritz ◽  
H W Moser ◽  
D A Wenger ◽  
E H Kolodny
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Plasma Exchange ◽  
Chromatographic Analysis ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Urine Sediment ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract Quantitative high-performance liquid chromatographic analysis of perbenzoylated sphingolipids has been used to study the correlations of body chemistry to clinical phenomena. Plasma sphingolipids were isolated from 32 Gaucher (β-glucosidase deficiency) and six Fabry (α-galactosidase deficiency) patients by solvent partition and chromatographic separation on silicic acid columns. Plasma sphingolipids from a patient undergoing plasma-exchange were separated from interfering lipids with reversed-phase columns. Liquid-chromatographic analysis of sphingolipids provides useful supportive information for diagnoses because affected individuals are shown to possess increased circulating concentrations of the pathognomonic sphingolipid. We also used this technique to monitor sphingolipid concentrations in plasma and urine sediment during plasma exchange of a p atient with Fabry’s disease. Regular plasma exchanges produced and maintained decreased concentrations of sphingolipids in plasma, but near pre-exchange concentrations were observed within days after the therapy was terminated.

Dissolvable Mg/Al layered double hydroxides and surfactant as an extractant for trace analysis of benzoylurea insecticides by high performance liquid chromatography

2020 ◽  
Vol 12 (44) ◽  
pp. 5380-5391
Author(s):  
Yanawath Santaladchaiyakit ◽  
Supalax Srijaranai
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chromatographic Analysis ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Analysis ◽  
Benzoylurea Insecticides ◽  
Double Hydroxides ◽  
Liquid Chromatographic ◽  
Preconcentration Method

A simple and rapid preconcentration method using dissolvable Mg/Al LDHs and SDS has been demonstrated for high performance liquid chromatographic analysis of benzoylurea insecticides in water and honey samples.

Analysis for glutathione in blood by high-performance liquid chromatography.

1978 ◽  
Vol 24 (7) ◽  
pp. 1140-1143 ◽  
Author(s):  
D L Rabenstein ◽  
R Saetre
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Colorimetric Assay ◽  
High Performance Liquid Chromatographic ◽  
Electrochemical Detector ◽  
Nitrobenzoic Acid ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract A high-performance liquid chromatographic method is presented for determination of glutathione in whole blood. Sample preparation involves hemolysis, protein precipitation, centrifugation, and filtration. The glutathione in the filtrate is then separated from other sulfhydryl-containing molecules by liquid chromatography with Zipax SCX cation-exchanger followed by detection with a mercury-based electrochemical detector. The liquid-chromatographic analysis time is approximately 5 min. Because of the chromatographic separation and the selectivity of the detector, the detection step is free from interferences from other components of blood. The method has been checked by comparison with the colorimetric assay based on reaction with 5,5'-dithiobis(2-nitrobenzoic acid). The chromatographic results are consistently slightly lower, presumably because of the greater selectivity of this method.

Nitrile-Terminated Hydrocarbons as Stationary Phases for High-Performance Liquid Chromatography of Steroid Hormones

1973 ◽  
Vol 19 (11) ◽  
pp. 1293-1295 ◽  
Author(s):  
Francis A Fitzpatrick
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Steroid Hormones ◽  
High Performance ◽  
Chromatographic Method ◽  
Stationary Phases ◽  
High Performance Liquid Chromatographic ◽  
Unsaturated Ketone ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A high-performance liquid chromatographic method for separating steroid hormones by using nitrile-terminated hydrocarbons as the stationary phase is described. Particular selectivity toward the separation of corticosteroids with an α, β unsaturated ketone group is noted, with five steroids more polar than cortisol being completely resolved. The system described is also applicable to estrogen separations.

Lactate dehydrogenase inhibitors in NADH preparations.

1977 ◽  
Vol 23 (9) ◽  
pp. 1581-1584 ◽  
Author(s):  
S A Margolis ◽  
B F Howell ◽  
R Schaffer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Lactate Dehydrogenase ◽  
High Performance ◽  
Chromatographic Method ◽  
Deae Cellulose ◽  
High Performance Liquid Chromatographic ◽  
Clinical Assay ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract The presence of a new lactate dehydrogenase inhibitor on the trailing edge of the NADH peak from chromatography on diethylaminoethyl-celluose [Loshon et al., Clin. Chem., this issue] was verified. It was resolved from the NADH by high-performance liquid chromatography on muBondapak C18. When the new inhibitor was present in a reaction mixture to the extent that, of the initial 260-nm absorbance, about 5% was contributed by the inhibitor, the rate of NADH oxidation by lactate dehydrogenase decreased by 65%. The inhibitor absorbs at 260 and 340 nm, and is different from the Strandjord-Clayson inhibitor [J. Lab. Clin. Med. 67, 144 (1966)] by both types of chromatography. Because this new inhibitor has ultraviolet properties similar to those of NADH and chromatographs with the NADH on DEAE-cellulose, the high-performance liquid chromatographic method must be used to ensure its absence in preparations of NADH used for clinical assay.

Liquid-chromatographic analysis for methylphenidate (Ritalin) in serum.

1979 ◽  
Vol 25 (3) ◽  
pp. 401-404 ◽  
Author(s):  
S J Soldin ◽  
Y P Chan ◽  
B M Hill ◽  
J M Swanson
Keyword(s):  
Chromatographic Analysis ◽  
High Performance ◽  
Plasma Sample ◽  
Potassium Phosphate ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Column Temperature ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract We describe a "high performance" liquid chromatographic method for quantitating methylphenidate in serum. The internal standard, 4,5-diphenylimidazole, and serum or plasma sample are extracted in chloroform, evaporated, and redissolved in 20 mmol/L potassium phosphate (pH 3.5)/high-purity acetonitrile, 80/20 by vol. A centrifuged aliquot is chromatographed on mu-Bondapak C-18 with the phosphate/acetonitrile solvent as mobile phase, a flow rate of 1.6 mL/min, and a column temperature of 40 degrees C. Absorbances are read at 192 nm. This method reliably measures concentrations greater than 20 micrograms/L and has analytical recoveries of 74%.

High Performance Liquid Chromatographic Analysis of Hydrocortisone Acetate Ointments: Interlaboratory Study

1981 ◽  
Vol 64 (4) ◽  
pp. 870-874 ◽  
Author(s):  
David M Hailey ◽  
Anthony R Lea
Keyword(s):  
High Performance ◽  
Total Error ◽  
High Performance Liquid Chromatographic ◽  
Interlaboratory Study ◽  
Hydrocortisone Acetate ◽  
Liquid Chromatographic Method ◽  
Two Samples ◽  
Error Standard Deviation ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract An interlaboratory study was carried out on a high performance liquid chromatographic method for determining hydrocortisone acetate in ointments. The method represents an alternative to the colorimetric procedure of the British Pharmacopoeia. Two samples of a commercially available hydrocortisone acetate ointment were analyzed by 14 laboratories. Column performance and precision of the assay were satisfactory. The total error standard deviation for the method was 3.69%.

pplicability of a Multiresidue Method and High Performance Liquid Chromatography for Determining Quinomethionate in Apples and Oranges

1983 ◽  
Vol 66 (4) ◽  
pp. 1018-1022
Author(s):  
Richard T Krause ◽  
E Michael August
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pesticide Residues ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Fluorescence Detector ◽  
Multiresidue Method ◽  
Liquid Chromatographic ◽  
Ppm Level

Abstract The AOAC multiresidue method for nonpolar pesticide residues in nonfatty foods has been coupled with a high performance liquid chromatographic (HPLC)-fluorometric system for determining quinomethionate residues on apples and oranges. Quinomethionate is extracted with acetonitrile, and coextractives are removed with liquid-liquid partitioning and Florisil adsorbent using the AOAC multipesticide residue method for nonfatty foods. The quinomethionate fraction is then chromatographed on an HPLC octyl-bonded column and detected in-line with a fluorescence detector using 362 nm excitation and 395 nm emission. Recovery studies were conducted with apples fortified with quinomethionate at 0.05 ppm and oranges at 0.05 and 0.5 ppm. The recoveries averaged 100% (range 92-108) at the 0.05 ppm fortification level and 102% (range 93-110) at the 0.5 ppm level.

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