Clinical Evaluation of a Rapid Screening Test for Sickle Cell Trait (S[unknown]) and Sickle Cell Anemia (SS)

Abstract The need for a rapid screening procedure that differentiates both sickle-cell trait (S[unknown]) and sickle-cell anemia (SS) in a single test system has led to the development of a new 10-min screening test. The "Sickle-I.D." system is based on a modification of the sodium dithionite hemoglobin solubility test. It is composed of three reagents and requires 0.1 ml of whole blood. Two independent blind clinical comparisons of this new procedure with hemoglobin electrophoresis (4328 samples) revealed an accuracy of 99.8% in identification and differentiation of sicklecell trait and sickle-cell anemia. Together with confirmation of positives by hemoglobin electrophoresis, this new procedure provides rapid and accurate screening for sickle-cell trait (S[unknown]) and sickle-cell anemia (SS).

Download Full-text

Rapid Solubility Test for Detection of Hemoglobin S

Clinical Chemistry ◽

10.1093/clinchem/17.11.1081 ◽

1971 ◽

Vol 17 (11) ◽

pp. 1081-1082 ◽

Cited By ~ 4

Author(s):

Jean E Matusik ◽

James B Powell ◽

David M Gregory

Keyword(s):

Phosphate Buffer ◽

Screening Test ◽

Rapid Screening ◽

Hemoglobin S ◽

Solubility Test ◽

Negative Results ◽

Rapid Screening Test

Abstract We have devised a rapid screening test for use in detecting sickling hemoglobins. This test depends on the insolubility of sickling hemoglobins in 2.24 molar phosphate buffer. Sickling hemoglobins form a turbid suspension in this test, in which positive and negative results, respectively, appear very much like those for the "Sickledex" test.

Download Full-text

Rapid Screening Test for the Evaluation of Quaternary Ammonium Compounds by Triphenyltetrazolium Chloride Reduction by Microorganisms

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/53.6.1224 ◽

1970 ◽

Vol 53 (6) ◽

pp. 1224-1228

Author(s):

Raymond J Sitkiewicz

Keyword(s):

Quaternary Ammonium ◽

Screening Test ◽

Rapid Test ◽

Test System ◽

Quaternary Ammonium Compounds ◽

Rapid Screening ◽

Ammonium Compound ◽

Triphenyltetrazolium Chloride ◽

Rapid Screening Test ◽

Ammonium Compounds

Abstract A rapid screening test has been developed to determine killing dilutions of quaternary ammonium compounds. Two factors, Letheen broth as a quaternary ammonium compound neutralizer and triphenyltetrazolium chloride as a microbial growth indicator, resulted in a rapid test system for the evaluation of quaternary ammonium compounds. This rapid test system takes from 4 to 8 hr to complete as opposed to the 24 to 48 hr for other tests. A new germicide can be bacteriologically screened in one day.

Download Full-text

Rapid screening test for porphyrinuria

Archives of Dermatology ◽

10.1001/archderm.102.2.237 ◽

1970 ◽

Vol 102 (2) ◽

pp. 237-237

Author(s):

R. M. McDonald

Keyword(s):

Screening Test ◽

Rapid Screening ◽

Rapid Screening Test

Download Full-text

Rapid screening test for adulteration in raw materials of dietary supplements

Vibrational Spectroscopy ◽

10.1016/j.vibspec.2010.11.009 ◽

2011 ◽

Vol 55 (2) ◽

pp. 216-223 ◽

Cited By ~ 38

Author(s):

Andrea B. Champagne ◽

Katrina V. Emmel

Keyword(s):

Dietary Supplements ◽

Screening Test ◽

Raw Materials ◽

Rapid Screening ◽

Rapid Screening Test

Download Full-text

A Rapid Screening Test for Detection of Poisons in Blood

Nature ◽

10.1038/1931298a0 ◽

1962 ◽

Vol 193 (4822) ◽

pp. 1298-1298 ◽

Cited By ~ 2

Author(s):

H. V. STREET

Keyword(s):

Screening Test ◽

Rapid Screening ◽

Rapid Screening Test

Download Full-text

PATHOGENESIS OF HYPOSTHENURIA IN PERSONS WITH SICKLE CELL ANEMIA OR THE SICKLE CELL TRAIT

PEDIATRICS ◽

10.1542/peds.26.2.249 ◽

1960 ◽

Vol 26 (2) ◽

pp. 249-254

Author(s):

L. Schlitt ◽

H. G. Keitel

Keyword(s):

Sickle Cell Disease ◽

Sickle Cell ◽

Sickle Cell Anemia ◽

Renal Damage ◽

Sickle Cell Trait ◽

Urinary Concentration ◽

Cell Disease ◽

Renal Vessels

Hyposthenuria was investigated in subjects with sickle cell trait and in patients with sickle cell anemia. The following were observed: 1) in subjects with sickle cell trait both normal and reduced maxima of urinary concentration are found, whereas all untreated patients with sickle cell anemia over 6 months of age have hyposthenuria; 2) hyposthenuria becomes increasingly more severe with advancing age in both sickle cell anemia and sickle cell trait; 3) in a 6-month-old patient with sickle cell anemia and hyposthenuria, the maxima of urinary concentration returned to normal after two transfusions of normal erythrocytes. Reasons are presented for favoring the hypothesis that hyposthenuria in sickle cell disease is due to renal damage, possibly from intravascular sickling of erythrocytes in renal vessels or from the presence of "free" circulating S-hemoglobin.

Download Full-text