Measurement of serum magnesium with a centrifugal analyzer.

1977 ◽  
Vol 23 (2) ◽  
pp. 289-291 ◽  
Author(s):  
H Khayam-Bashi ◽  
T Z Liu ◽  
V Walter
Keyword(s):  
Complex Formation ◽  
Atomic Absorption Spectroscopy ◽  
Serum Magnesium ◽  
Correlation Coefficients ◽  
Small Specimen ◽  
Reaction Volume ◽  
Correlation Studies ◽  
Analytical Recovery ◽  
Methylthymol Blue ◽  
Final Reaction Volume

Abstract We show how serum magnesium can be determined with a centrifugal analyzer. The method is based upon a manual procedure involving magnesium/calmagite complex formation in an alkaline reagent mixture. The assay, performed at 30 degrees C, requires 5 mul of serum in a final reaction volume of 405 mul, including 350 mul of reagent mixture. The stable color produced at 1 min is measured at 520 nm. Linearity studies showed that Beer's law was followed to 50 mg/liter. Average analytical recovery was 97.3%. Within-day studies showed CV's of 3.36% and 1.09%, compared to day-to-day variations of 5.32% and 3.04% at 18.8 and 46.6 mg of magnesium per liter, respectively. Correlation studies with the manual method, the Du Pont aca (methylthymol blue compleximetric) procedure, and atomic absorption spectroscopy showed correlation coefficients of 0.996, 0.976, and 0.950, respectively. Results compare excellently with those by common present methods. The method is fast, economical, reliable, and applicable to small specimen volumes.

scholarly journals An alternative method for Staphylococcus aureus DNA isolation

2008 ◽  
Vol 60 (2) ◽  
pp. 299-306 ◽  
Author(s):  
L. Chapaval ◽  
D.H. Moon ◽  
J.E. Gomes ◽  
F.R. Duarte ◽  
S.M. Tsai
Keyword(s):  
Staphylococcus Aureus ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Environmental Isolates ◽  
Reaction Volume ◽  
High Quality ◽  
Rapid Procedure ◽  
Final Reaction ◽  
Staphylococcal Enterotoxins ◽  
Final Reaction Volume

This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.

scholarly journals An Investigation on Serum Mineral Levels of Healthy Norduz and Hair Goats Raised in Semi-Intensive Conditions

2020 ◽  
Vol 8 (8) ◽  
pp. 1795-1802
Author(s):  
Ayşe Özge Demir ◽  
Ferda Karakuş ◽  
Suna Akkol
Keyword(s):  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Age Groups ◽  
Correlation Coefficients ◽  
Serum Minerals ◽  
Significant Difference ◽  
Positive Correlations ◽  
Mineral Levels ◽  
Ppm Level

The aim of this study was to determine the some serum minerals and their interactions between in 2, 3 and 4 years-old healthy Norduz (n=45) and Hair (n=31) goats raised in semi-intensive conditions. Mineral levels were determined with Atomic absorption spectroscopy (AAS) in ppm level. Results were calculated as Fe 1.578±0.088 and 1.379±0.095 mmol/L, Cu 1.300±0.067 and 1.303±0.080 mg/L, Zn 0.972±0.029 and 0.937±0.029 mg/L, K 4.574±0.091 and 2.102±0.074 mmol/L, Mg 2.089±0.057 and 4.670±0.098 mmol/L, Mn 2.163±0.152 and 2.215±0.198 mg/L, Pb 0.078±0.005 and 0.087±0.006 mg/L for Norduz and Hair goats, respectively. While the differences in the mineral levels of hair goats were not significant, significant differences has been found between the age groups in terms of K, F and Pb in Norduz goats. In addition, while there was no statistically significant difference between 3-year-old goats, statistically significant differences hs been found for Fe and 2-year-old goats K and Mg in 4-year-old goats. Moreover, with respect to correlation coefficients, positive correlations were obtained both between K-Mg at Norduz goats and between Fe-Cu, Fe-K, Fe-Mg, K-Mg at Hair goats in all years-old groups.

Evaluation of a modified alcohol dehydrogenase assay for the determination of ethanol in blood.

1982 ◽  
Vol 28 (10) ◽  
pp. 2125-2127 ◽  
Author(s):  
A Poklis ◽  
M A Mackell
Keyword(s):  
Gas Chromatography ◽  
Reaction Time ◽  
Alcohol Dehydrogenase ◽  
Correlation Coefficients ◽  
Least Square ◽  
Standard Curve ◽  
Analytical Recovery ◽  
Sigma Chemical ◽  
Enzymic Assay

Abstract We evaluated a new alcohol dehydrogenase (EC 1.1.1.1) enzymic assay (ADH-glycine, Sigma Chemical Co.) for the determination of ethanol in blood. This assay differs from the manufacturer's previous assay (ADH-pyrophosphate) in that glycine replaces pyrophosphate as the buffer and hydrazine replaces semicarbazide as the trapping agent. The standard curve for the assay was linear over blood ethanol concentrations of 0.50-5.00 g/L. The reaction time of the assay was 10 min. At 1.00 g/L within-run and between-run CVs were 3.96% (n = 20) and 4.01% (n = 20), respectively. Mean analytical recovery of ethanol added to whole blood at 0.50-5.00 g/L was 99.7% (SD 2.6%). We performed 100 consecutive clinical and forensic determinations by the ADH-glycine assay, the ADH-pyrophosphate assay, and gas chromatography. Correlation coefficients of the results by least-square linear regression were 0.995 for ADH-pyrophosphate vs ADH-glycine, and 0.990 for gas chromatography vs ADH-glycine. The major advantage of the ADH-glycine assay over the ADH-pyrophosphate assay is the shorter reaction time, 10 min vs 30 min.

Spectrophotometric screening method for acetaminophen in serum and plasma.

1980 ◽  
Vol 26 (1) ◽  
pp. 69-71
Author(s):  
T Z Liu ◽  
K H Oka
Keyword(s):  
Free Radical ◽  
Screening Method ◽  
Correlation Coefficients ◽  
Acidic Ph ◽  
Correlation Studies ◽  
Toxic Concentrations

Abstract We describe a simple, economical procedure for rapidly detecting acetaminophen in serum or plasma. The method is based upon the reduction by the drug of ferric 2,4,6-tris(2-pyridyl)-s-triazine, at an acidic pH, to ferrous 2,4,6-tris(2-pyridyl)-S-triazine complex, which absorbs maximally at 593 nm. Absorbance and acetaminophen concentration are linearly related from 25 to 400 mg/L, and so therapeutic and toxic concentrations can be measured. The method is accurate; day-to-day CV's for two pooled control specimens (103 and 227 mg/L) were 4.4 and 6.6%. Correlation studies, with an established nitration method and with the free-radical diphenylpicrylhydrazyl dye method, showed correlation coefficients of 0.985 and 0.915, respectively. Of 25 commonly used drugs tested, only levodopa, oxyphenylbutazone, and phenylephrine interfere significantly. Interference from salicylate, salicylamide, and phenylbutazone was insignificant.

Dry- and wet-ashing techniques compared in analyses for zinc, copper, manganese, and iron in hair.

1986 ◽  
Vol 32 (5) ◽  
pp. 739-742 ◽  
Author(s):  
J K Friel ◽  
C D Ngyuen
Keyword(s):  
Trace Metal ◽  
Atomic Absorption ◽  
Atomic Absorption Spectroscopy ◽  
Suitable Alternative ◽  
Analytical Recovery ◽  
Hair Samples ◽  
Copper Manganese ◽  
Dry Ashing ◽  
Wet Ashing ◽  
Iron And Manganese

Abstract Preparation of hair specimens for trace-metal analyses is routinely done by wet- or dry-ashing. Wet-ashing is more time consuming than dry-ashing and can be dangerous. We wished to determine if dry-ashing was a suitable alternative to wet-ashing with HClO4:HNO3 or HNO3 alone in preparing hair for measurement of zinc, copper, iron, and manganese by atomic absorption spectroscopy. Concentrations of Zn, Cu, and Mn were not differently affected in hair that was dry- or wet-ashed. Analytical recovery of these elements added to hair samples ranged from 102 to 108%; day-to-day CVs were less than 5%. Fe was lost during dry-ashing of hair, and wet-ashing with HNO3 produced results for Fe comparable with those obtained with HClO4:HNO3. Therefore we recommend dry-ashing of hair to be analyzed for Zn, Cu, and Mn, but wet-ashing with HNO3 for assays of Fe.

Rate enhancement of the nickel(II)-pada complex formation in sodium alkanesulfonate micellar solutions

1982 ◽  
Vol 35 (1) ◽  
pp. 15 ◽  
Author(s):  
JR Hicks ◽  
VC Reinsborough
Keyword(s):  
Complex Formation ◽  
Chain Length ◽  
Micellar Solution ◽  
Binding Constants ◽  
Bidentate Ligand ◽  
Kinetic Constants ◽  
Micellar Solutions ◽  
Reaction Volume ◽  
Rate Of Reaction ◽  
Alkyl Sulfates

Sodium alkanesulfonates (C9, C8, C7, and C6) in micellar solution have been used to enhance the rate of reaction between Niaq2+ and the bidentate ligand pyridine-2-azo-p-dimethylaniline (pada) at 298.2 K. A Berezin-like model has been used to account for the observed kinetic constants in terms of the binding constants of each of the reagents to the micelles and the reaction volume and the concentration of the micellar surfactant. For a given chain length, the alkanesulfonates are about 25 % less effective than alkyl sulfates in promoting the rate of reaction and the model accounts for this successfully.

Serum magnesium determined by use of methylthymol blue.

1987 ◽  
Vol 33 (4) ◽  
pp. 614-615 ◽  
Author(s):  
P Thuvasethakul ◽  
W Wajjwalku
Keyword(s):  
Serum Magnesium ◽  
Methylthymol Blue
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