Optimized atomic absorption spectrophotometry of zinc in cerebrospinal fluid.

1983 ◽  
Vol 29 (3) ◽  
pp. 486-491 ◽  
Author(s):  
R Palm ◽  
R Sjöström ◽  
G Hallmans
Keyword(s):  
Cerebrospinal Fluid ◽  
Atomic Absorption ◽  
Direct Determination ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Zn Concentration ◽  
Insignificant Difference ◽  
The Mean

Abstract This method for direct determination of Zn in cerebrospinal fluid (CSF) involves flame atomic absorption spectrophotometry with a pulse nebulizer technique. Standard solutions of Zn in 150 mmol/L NaCl were used. We could account for 88% of added standard with the method in individual samples from 10 patients and in pooled CSF. The method is acceptably precise, CVs in pooled CSF ranging from 4 to 12%. The mean CSF-Zn concentration for nine healthy men was 0.18 (SD 0.04) mumol/L and for nine healthy women 0.15 (SD 0.03) mumol/L, a statistically insignificant difference. These values are lower than those in previous reports, which may have been the result of contamination problems, nonatomic absorption, or nonstandardized sampling. In the healthy volunteers, the CSF-Zn concentration was positively correlated with serum-Zn, CSF-protein, and CSF-albumin concentrations, as well as with the CSF/serum ratio for albumin.

Influence of Phosphorus and Calcium on Flame Atomic Absorption Spectrophotometric Determination of Lead in Canned Fish Products

1984 ◽  
Vol 67 (1) ◽  
pp. 186-187
Author(s):  
Teresa M Serra ◽  
J F Vale Serrano
Keyword(s):  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Flame Atomic Absorption Spectrophotometry ◽  
Experimental Conditions ◽  
Fish Products ◽  
Real Samples ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Canned Fish

Abstract Lead was determined in the presence of whole multiples of the P/Ca ratio found in Portuguese canned fish by flame atomic absorption spectrophotometry with and without using an ashing aid. Under our experimental conditions, use of the ashing aid eliminates P and Ca interference. Results with real samples, spiked with 1, 2,3, and 4 ppm lead, are presented and statistically treated.

Optimized atomic absorption spectrophotometry of calcium in erythrocytes.

1987 ◽  
Vol 33 (11) ◽  
pp. 2004-2007 ◽  
Author(s):  
S Nomoto ◽  
S Shoji
Keyword(s):  
Hydrochloric Acid ◽  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Graphite Tube ◽  
Optimum Conditions ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Flame Atomic Absorption Spectrophotometer ◽  
The Mean ◽  
Mean Concentration

Abstract We sought to establish optimum conditions for measuring calcium in erythrocytes by atomic absorption spectrophotometry. The conditions we selected are as follows. Wash one volume of fresh heparin-treated packed cells once with 30 volumes of isotonic buffered saline (pH 7.4) at a temperature somewhat exceeding 25 degrees C. Dilute the washed packed cells 10-fold with 12 mmol/L hydrochloric acid, and analyze the supernate for calcium. Measure the hematocrit of the washed packed cells, then analyze an aliquot of them for calcium, using a computer-readout type of flame or a non-flame atomic absorption spectrophotometer equipped with a pyrocoated graphite tube. The temperature program is 1000 degrees C for ashing [corrected] and 1800 degrees C for the atomizing cycle. Intraday and day-to-day reproducibility of the assay was 6.55% and 8.19%, respectively, at the mean concentration of calcium in the erythrocytes of healthy adults, which is 4.30 mumol/L.

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