Interference by hemolysis with various methods for total calcium and its correction by trichloroacetic acid precipitation.

1983 ◽  
Vol 29 (8) ◽  
pp. 1497-1500 ◽  
Author(s):  
K M Chan ◽  
C Arriaga ◽  
M Landt ◽  
C H Smith ◽  
R H Ng
Keyword(s):  
Atomic Absorption ◽  
Trichloroacetic Acid ◽  
Acid Treatment ◽  
Acid Precipitation ◽  
Total Serum ◽  
Direct Reaction ◽  
Positive Bias ◽  
Total Calcium ◽  
Serum Samples ◽  
Total Serum Calcium

Abstract We evaluated the influence of hemolysis on total serum calcium as determined with the Du Pont aca, SMA 12/60, Ektachem 400, Corning 940 EGTA titrator, and the Beckman Astra 8, comparing results with those obtained by atomic absorption spectroscopy. We find that hemoglobin does not influence calcium measurements with the SMA 12/60, Ektachem 400, or Beckman Astra 8. The presence of hemoglobin exceeding 2 to 3 g/L caused falsely high results with the aca and falsely low results with the Corning 940 titrator. Similar interferences may be observed with other titrating or colorimetric procedures that involve direct reaction of the sample with o-cresolphthalein complexone or calcein. Upon removal of the hemoglobin by precipitation with trichloroacetic acid, the values obtained with the aca and the Corning 940 EGTA titrator were similar to those measured by atomic absorption. With nonhemolyzed serum samples the acid treatment had little or no effect on the aca procedure but resulted in a positive bias of approximately 10% with the EGTA procedure. Thus we recommend this trichloroacetic acid procedure for measuring calcium in hemolyzed samples with the aca and, with certain reservations, with the EGTA titrator.

scholarly journals HYPOCALCEMIA AFTER PARATHYROIDECTOMY

2020 ◽  
Vol 23 (2) ◽  
pp. 75-79
Author(s):  
L. P. Kotelnikova ◽  
G. Yu. Mokina ◽  
N. G. Polyakova
Keyword(s):  
Parathyroid Hormone ◽  
Calcium Level ◽  
Clinical Symptoms ◽  
Clinical Signs ◽  
Total Serum ◽  
Primary Group ◽  
Total Calcium ◽  
Baseline Serum ◽  
Median Baseline ◽  
Total Serum Calcium

The aim of the study was to estimate the frequency and timing of hypocalcemia after surgical treatment for primary, secondary and tertiary hyperparathyroidism. Materials and methods. 21 patients were operated for hyperparathyroidism, 15 - for primary (group 1), 6 - for secondary and tertiary (group 2). In I group the median baseline level of total serum calcium was 3.06 mmol/l, phosphorus0.9 mmol/l, and parathyroid hormone360 pmol/l. In II group all patients were on program dialysis for end-stage chronic kidney failure for at least five years. The median baseline serum total calcium level was 2.29 mmol/l, phosphorus2.64 mmol/l, and parathyroid hormone-1822 pmol/l. Results. A day after removal of the parathyroid adenoma (1 group) the level of calcium and phosphorus was normalized, the content of parathyroid hormone (median 21.4 pmol/l) significantly decreased. In one case (6.7%) on the fifth day there were clinical signs of hypocalcemia and the level of calcium decreased to 1.86 mmol/l. All patients of the second group underwent subtotal parathyroidectomy. After a day the level of parathyroid hormone significantly decreased (median227 pmol/l). The phosphorus content has returned to normal. The calcium level in all cases exceeded 2 mmol/l. On day 4-5 the total calcium content decreased and ranged from 1.14 mmol/l to 2.04 mmol/l. Four patients (66,7%) showed clinical signs of hypocalcemia. It was found that the development of hypocalcemia has a positive correlation of average value with the level of parathyroid hormone, phosphorus and negative with the content of calcium before surgery. Conclusion. The decrease in the level of total calcium with the development of clinical symptoms occurs on 4-5 days after surgery for primary hyperparathyroidism in 6.7% and for secondary or tertiary - in 66.7%. Risk factors for hypocalcemia are the baseline low level of calcium and high of parathyroid hormone, phosphorus.

Biological and Analytic Components of Variation in Long-Term Studies of Serum Constituents in Normal Subjects

1971 ◽  
Vol 17 (10) ◽  
pp. 983-987 ◽  
Author(s):  
Eugene K Harris ◽  
David L DeMets
Keyword(s):  
Normal Individual ◽  
Normal Subjects ◽  
Total Serum Protein ◽  
Total Serum ◽  
Total Calcium ◽  
Intraindividual Variation ◽  
Total Serum Calcium ◽  
Normal Individuals ◽  
Long Term Studies

Abstract Intra- and interindividual components of variation in ionized calcium among normal individuals have been estimated. The basic data were means of duplicate analyses of total serum calcium, total serum protein, and serum albumin from 68 normal subjects, 10-12 weekly samples per person. The McLean-Hastings equation was used to estimate [Ca2+]. Use of observed albumin/globulin ratios, instead of an assumed constant, had negligible effect on mean [Ca2+] or components of variation. The interindividual component of variation in [Ca2+] was found to be the same as that in total calcium: 3%. Average intraindividual variation (0.045-0.05 mmol/liter), appeared to be entirely attributable to analytical deviations in total protein and total calcium determinations. Thus, in the average normal individual, no physiologic variation in [Ca2+] could be detected. Results agreed with recent data on [Ca2+] measured by calcium ion-selective electrodes.

scholarly journals To Study the Association of Total Serum Calcium Level in Assessing the Severity of Pancreatitis

2021 ◽  
pp. 781-785
Author(s):  
V. Lingesh Kumar ◽  
Saravanan Sundaram
Keyword(s):  
Acute Pancreatitis ◽  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Tertiary Care ◽  
Total Serum ◽  
Total Calcium ◽  
Blood Calcium ◽  
Total Serum Calcium ◽  
Total Serum Calcium Level

Background: Pancreatitis is an inflammatory condition that affects the pancreas and may also affect other tissues or distant organ. In this study our aim is to see if blood calcium level can be used as a prognostic marker in determining the severity of acute pancreatitis because hypocalcaemia is linked to higher mortality and hospitalization. Method: A retrospective study was conducted in the medical records department of a tertiary care health facility. A total of 75 participants were included in the research. .demographic details of all patients were collected .Patients above 18 years who presented with of epigastric pain, pre-Diagnosed gallstones, alcoholism were included int this study. The lowest total calcium levels were collected in order to examine total calcium as a predictive factor for severity. All of these information were collected and analyzed to get the appropriate findings. Results: A total of 75 patients were included in the research. The age of patients in each severity grade did not differ significantly (p value>0.05). The gender distribution of the two groups did not differ. (P >0.05). For mild and severe acute pancreatitis, mean total calcium levels were 7.98 and 6.67, respectively, which was statistically significant (p< 0.05). Conclusion: The total serum calcium level is a useful indicator for assessing  pancreatitis.

Free Calcium in Serum. II. Rigor of Homeostatic Control, Correlations with Total Serum Calcium, and Review of Data on Patients with Disturbed Calcium Metabolism

1973 ◽  
Vol 19 (6) ◽  
pp. 575-582 ◽  
Author(s):  
Jack H Ladenson ◽  
George N Bowers
Keyword(s):  
Correlation Coefficient ◽  
Calcium Metabolism ◽  
Calcium Concentration ◽  
Mean Value ◽  
Total Serum ◽  
Free Calcium ◽  
Total Calcium ◽  
Disease States ◽  
Total Serum Calcium ◽  
Normal Individuals

Abstract Two hundred and fifteen measurements of free (ionic) and total calcium were performed on sera from 184 patients in whom some disturbance in calcium metabolism was suspected. The correlation coefficient between free and total calcium was 0.94, but a scattergram indicated that free calcium concentration cannot be predicted from knowledge of total calcium concentration. In "normal" individuals the correlation coefficient was only 0.45. Homeostasis of free and total calcium was examined by making periodic measurements in seven normal individuals over a period of eight months; the variability around the individual’s mean value was 1.2% for free calcium and 1.9% for total calcium. Free calcium, as properly measured by the ion-specific electrode, is a better measure of calcium status than is total calcium in the disease states studied, which were mostly hyperparathyroidism, multiple myeloma, and renal failure.

Concurrent determination of total serum calcium and magnesium by thermometric titration with ethylenediaminetetraacetate.

1976 ◽  
Vol 22 (7) ◽  
pp. 1084-1088 ◽  
Author(s):  
R H Callicott ◽  
P W Carr
Keyword(s):  
Atomic Absorption ◽  
Serum Calcium ◽  
Atomic Absorption Spectroscopy ◽  
Total Serum ◽  
Total Serum Calcium ◽  
Constant Rate ◽  
Thermometric Titration ◽  
Calcium And Magnesium ◽  
Disodium Ethylenediaminetetraacetate

Abstract Total serum calcium and magnesium may be determined in one thermometric titration, with disodium ethylenediaminetetraacetate as the titrant. A 1-ml serum sample is diluted with 1 ml of tris(hydroxymethyl)aminomethane buffer (pH 8) and titrated at a constant rate with a motorized syringe buret. Results by the thermometric method compared well with those by atomic absorption spectroscopy.

scholarly journals Total Serum Level of Calcium and Ion Calcium is Lower in Hypotonic Uterine Inertia

2016 ◽  
pp. 145-148
Author(s):  
Josef Wattimury ◽  
Wiryawan Permadi ◽  
Edwin Armawan
Keyword(s):  
Serum Level ◽  
Correlation Coefficient ◽  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Total Serum ◽  
Total Calcium ◽  
Uterine Contractility ◽  
Total Serum Calcium ◽  
Uterine Inertia

Objectives: To know the correlation between total and ion serum calcium level with the uterine contractility of laboring patient. Methods: This is a cross-sectional analytic correlative study measuring the strength of correlation of total and ion calcium serum level between groups with hypotonic uterine inertia - and normal labor. Each group consists of 20 subjects who met inclusion criteria and presented to Dr. Hasan Sadikin Hospital in August - September 2012. Statistical analysis was performed by using Eta (η) coefficient. Results: Mean total calcium serum level on hypotonic uterine inertia is 6.66 mg/dl, while mean of total calcium serum level on normal labor is 8.56 mg/dl, with Eta (η) correlation coefficient 0.721. Mean ion calcium serum level on hypotonic uterine inertia is 4.14 mg/dl, while mean of total calcium serum level on normal labor is 4.92 mg/dl, with Eta (η) correlation coefficient 0.802. Conclusion: Total serum level of calcium and ion calcium in hypotonic uterine inertia is lower than the level of which in normal labor. There is a strong correlation between total and ion serum calcium level with uterine contractility, the Eta (η) correlation coefficient are 0.721 and 0.802 respectively. [Indones J Obstet Gynecol 2013; 1-3: 145-8] Keywords: hypotonic uterine inertia, ion serum calcium level, normal labor, total serum calcium level

scholarly journals SUN-LB67 Revisit Serum Calcium Correction

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Yulong Li ◽  
JunJia Zhu ◽  
Jenny Blau ◽  
William F Simonds
Keyword(s):  
Serum Calcium ◽  
Repeated Measures ◽  
Calcium Level ◽  
Ionized Calcium ◽  
Total Serum ◽  
P Value ◽  
Total Calcium ◽  
Total Serum Calcium ◽  
Parathyroid Disease ◽  
Calcium Status

Abstract Context: The serum calcium level is one of most routinely ordered tests in clinical practice. Many factors can affect calcium level and its interpretation. There are challenges and barriers in applying calcium correction formulas to every-day practice. Objective: Revisit correlation between total and ionized calcium levels, and dependence of serum calcium on albumin, pH and creatinine levels. Methods: This study included 1537 subjects enrolled in a parathyroid disease clinical protocol. We examined calcium and relevant biochemistry tests collected simultaneously and repetitively over consecutive years. Histograms, repeated measures correlation, correlation plots, and liner regression plots were used to analyze and visualize the data. Results: We found that: 1) directly measured total serum calcium and ionized calcium had excellent correlation and dependence with p-value=2.2e-16, repeated measures correlation coefficient (rmcorr)=0.919, and 95% interval (CI) = 0.916 to 0.922; 2) there was a low dependence between total serum calcium and albumin levels (rmcorr=0.454, 95% CI=0.433 to 0.474), a low dependence between ionized calcium and pH levels (rmcorr=-0.309, 95% CI= -0.326 to -0.292), and no dependence between total calcium and creatinine levels (rmcorr=0.026 95% CI=0.012 to 0.040); 3) using the commonly applied correction formulas, to either adjust total calcium based on albumin levels or else adjust ionized calcium based on pH levels, did not improve dependence among them. Conclusions: We therefore suggest using directly measured total serum calcium and/or ionized calcium level to assess clinical calcium status in general patients tested for parathyroid related disorders.

scholarly journals Total serum calcium and corrected calcium as a predictor of severity in acute pancreatitis

2018 ◽  
Vol 5 (11) ◽  
pp. 3558
Author(s):  
Kemparaj T. ◽  
Narasimhamurthy K. N. ◽  
Archit Muralidhar
Keyword(s):  
Acute Pancreatitis ◽  
Hospital Admission ◽  
Epigastric Pain ◽  
Tertiary Care ◽  
Previous History ◽  
Total Serum ◽  
P Value ◽  
Total Calcium ◽  
Total Serum Calcium ◽  
Significant Difference

Background: Acute pancreatitis is an acute inflammatory process of the pancreas. The Ranson and the modified Glasgow scores require 48 hours of data collection to asses’ severity. Hypocalcaemia is one of the components of Ranson's scoring system done to assess the severity of pancreatitis. Albumin-corrected calcium (ACC) has also been associated with severity, but no study has evaluated it as a prognostic severity factor within the first 24 h of the patient’s hospital admission. This study tries to evaluate total calcium and albumin corrected calcium as prognostic severity markers in acute pancreatitis within first 24 hour of admission.Methods: This prospective study was conducted in Bowring and Lady Curzon hospital, a tertiary care centre from June 2016 to May 2018. Inclusion criteria included all patients above 18 years of age, who presented within 72 hours of onset of epigastric pain. The clinical and demographic data with respect to gender, age, previous history of pancreatitis, total calcium taken 24 h after admission. In order to evaluate total calcium and albumin corrected calcium as prognostic factors of severity, the lowest total calcium values were collected within the first 24 h of hospital admission. These values were then corrected according to the serum albumin level.Results: A total of ninety-four patients were included in the study. There was no significant difference in the age of patients in each severity grade (p value: 0.242). No difference was observed in gender distribution of three groups. (P >0.05). As compared to total calcium, mean values of albumin corrected calcium were 8.03, 7.18 and 6.28 for mild, moderate and severe acute pancreatitis respectively, which were also significant at 0.05.Conclusions: Total calcium and albumin-corrected calcium obtained within the first 24 hours of hospital admission are useful predictors of severity in acute pancreatitis.

scholarly journals Duration and dose of antiepileptic drugs and serum calcium levels in children

2017 ◽  
Vol 57 (2) ◽  
pp. 104
Author(s):  
Arinta Atmasari ◽  
Masayu Rita Dewi ◽  
Aditiawati Aditiawati ◽  
Masagus Irsan Saleh
Keyword(s):  
Valproic Acid ◽  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Total Serum ◽  
Total Calcium ◽  
Total Serum Calcium ◽  
Carbamazepine Therapy ◽  
Epileptic Children ◽  
Total Serum Calcium Level

Background Antiepileptic drugs (AEDs) may affect calcium metabolism through several mechanisms. Much evidence has confirmed that carbamazepine and valproic acid, as the most widely used AEDs in epileptic children, leads to decreased serum calcium levels. This effect was suggested to be time and dose dependent. However, correlations between AEDs and calcium levels in Indonesian epileptic children have not been well studied.Objective To investigate possible correlations between total calcium levels and durations of therapy as well as doses of carbamazepine and valproic acid.Methods This analytical, cross-sectional study was performed from March to May 2015 in the Neuropediatric Outpatient Ward of Mohammad Hoesin Hospital, Palembang, South Sumatera. A total of 60 epileptic children taking carbamazepine and or valproic acid monotherapy were included and grouped accordingly. A single blood test was done for every participant to measure total serum calcium level. Correlation between daily dose or duration of AED with calcium level was assess using the Spearman-rho test.Results The mean total serum calcium levels in the carbamazepine and valproic acid groups were 9.48 (SD 0.83) mg/dL and 9.58 (SD 0.63) mg/dL, respectively. There was a statistically significant moderate correlation between the duration of carbamazepine therapy and total calcium level (r = 0.36; P=0.001). The cut-off point for duration of therapy was 23 months. There were no significant correlations between total calcium level and mean daily carbamazepine dose, nor between total calcium level and duration and dose of valproic acid therapy.Conclusion Longer duration of carbamazepine therapy is associated with low total serum calcium level, but carbamazepine dose is not. In addition, duration and dose of valproic acid are not associated with low total serum calcium level.

Sign in / Sign up

Export Citation Format

Share Document