Bence Jones proteinuria in multiple sclerosis.

1987 ◽  
Vol 33 (10) ◽  
pp. 1923-1924 ◽  
Author(s):  
A Pezzoli ◽  
E Pascali
Keyword(s):  
Multiple Sclerosis ◽  
Neurological Disorders ◽  
Healthy Subjects ◽  
Light Chains ◽  
Free Light Chains ◽  
Agarose Electrophoresis ◽  
Bence Jones Proteinuria ◽  
Bence Jones Proteins

Abstract We report our findings of Bence Jones proteins (monoclonal free light chains of immunoglobulins) in concentrated urines of patients with multiple sclerosis, by using agarose electrophoresis and immunofixation. The lack of such findings in urines from healthy subjects and patients with other neurological disorders should stimulate further investigation.

Kappa free light chains in multiple sclerosis – diagnostic accuracy and comparison with other markers

2021 ◽  
Vol 84/117 (4) ◽  
Author(s):  
Daniel Čierny ◽  
Ema Kantorová ◽  
Mária Škereňová ◽  
Egon Kurča ◽  
Ján Lehotský ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Diagnostic Accuracy ◽  
Light Chains ◽  
Free Light Chains

The contribute of cerebrospinal fluid free light-chain assay in the diagnosis of multiple sclerosis and other neurological diseases in an Italian multicenter study

2021 ◽  
pp. 135245852110641
Author(s):  
Gaetano Bernardi ◽  
Tiziana Biagioli ◽  
Paola Malpassi ◽  
Teresa De Michele ◽  
Domizia Vecchio ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Neurological Disorders ◽  
Operating Characteristic ◽  
Neurological Diseases ◽  
Characteristic Curve ◽  
Oligoclonal Bands ◽  
Free Light Chains ◽  
Complementary Information ◽  
Combined Use

Background: Cerebrospinal fluid (CSF) free light chains (FLCs) can be an alternative assay to oligoclonal bands (OCBs) in inflammatory neurological disorders, but threshold has no consensus. Objective: To assess the diagnostic accuracy of CSF FLCs in multiple sclerosis (MS) and other neurological diseases. Methods: A total of 406 patients from five Italian centers. FLCs were measured in CSF and serum using Freelite MX assays on Optilite. Results: A total of 171 patients were diagnosed as MS, 154 non-inflammatory neurological diseases, 48 inflammatory central nervous system (CNS) diseases, and 33 peripheral neurological diseases. Both kFLC and λFLC indices were significantly higher in patients with MS compared to other groups ( p < 0.0001). The kFLC index ⩾ 6.4 is comparable to OCB for MS diagnosis (area under the receiver operating characteristic curve (AUC) = 0.876; sensitivity 83.6% vs 84.2%; specificity 88.5% vs 90.6%). λFLC index ⩾ 5 showed an AUC of 0.616, sensitivity of 33.3% and specificity of 90.6%. In all, 12/27 (44.4%) MS patients with negative OCB had kFLC index ⩾ 6.4. Interestingly, 37.5% of 24 patients with a single CSF IgG band showed high kFLC index and 12.5% positive λFLC index. Conclusion: Our findings support the diagnostic utility of FLC indices in MS and other CNS inflammatory disorders, suggesting a combined use of FLC and OCB to help clinicians with complementary information.

Free light chains of immunoglobulins: clinical laboratory analysis

1994 ◽  
Vol 40 (10) ◽  
pp. 1869-1878 ◽  
Author(s):  
S S Levinson ◽  
D F Keren
Keyword(s):  
Direct Measurement ◽  
Clinical Laboratory ◽  
Great Sensitivity ◽  
Light Chains ◽  
Free Light Chains ◽  
Immunological Properties ◽  
Intrinsic Mechanism ◽  
Immunofixation Electrophoresis ◽  
Increased Sensitivity ◽  
Bence Jones Proteins

Abstract The increased sensitivity of immunofixation electrophoresis (IFE) over prior electrophoretic methods has led to renewed interest in the study of free light chains. Here, we discuss problems associated with the identification of monoclonal free light chains (Bence Jones proteins) in urine. Besides reviewing the nature of the sample specimens and the assays themselves, we discuss the physiology, biochemistry, genetics, and immunological properties of these molecules. Direct measurement of kappa/lambda ratios may ultimately be useful, but all commercial methods available now lack sufficient sensitivity. IFE is the preferred method because of its sensitivity and ease of interpretation. There are, however, difficulties associated with the interpretation of urinary IFE patterns, because the technique does not include an intrinsic mechanism for antibody-antigen titration and because of its great sensitivity in the absence of quantification. Problems of interpretation are discussed.

Contribution of Bence-Jones proteinuria to the monoclonal gammopathy diagnosis based on serum free light chains

2019 ◽  
Vol 493 ◽  
pp. S523
Author(s):  
D. Queimaliños Pérez ◽  
M. Rodríguez Mata ◽  
O. Ortiz Pastor ◽  
C. Pérez Portugués ◽  
B. Gutiérrez Cecchini ◽  
...  
Keyword(s):  
Monoclonal Gammopathy ◽  
Light Chains ◽  
Free Light Chains ◽  
Serum Free ◽  
Serum Free Light Chains ◽  
Bence Jones Proteinuria

scholarly journals Free Light Chains as a Novel Diagnostic Biomarker of Immune System Abnormalities in Multiple Sclerosis and HIV Infection

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Monika Gudowska-Sawczuk ◽  
Barbara Mroczko
Keyword(s):  
Multiple Sclerosis ◽  
Immune System ◽  
Hiv Infection ◽  
Light Chains ◽  
Laboratory Diagnostics ◽  
Elevated Concentration ◽  
Free Light Chains ◽  
Heavy Chains ◽  
Immune System Dysfunction ◽  
Pubmed Database

Introduction. Immunoglobulins are molecules composed of two heavy and two light chains. Light chains are produced by B lymphocytes during the synthesis of immunoglobulins, and physiologically light chains are generally produced in excess compared to heavy chains. Light chains that are not combined to heavy chains in a whole immunoglobulin are called free light chains (FLCs). B-cell abnormalities are associated with disorders leading to an abnormal concentration of free light chains. In this study, we focus on the described changes of serum and cerebrospinal fluid concentration of free light chains in inflammatory disorders: multiple sclerosis, HIV infection, and HIV-associated lymphomas. Methods. We performed broad research of the literature pertaining to our investigation via the MEDLINE/PubMed database. Results. It has been proven that FLC determination can provide rapid information about intrathecal inflammation in patients with multiple sclerosis. Moreover, literature data suggest that free light chain determination is the most interesting alternative for oligoclonal band analysis. In the present review, we also described that HIV-related immune system dysfunction is associated with an elevated concentration of serum-free light chains. Additionally, FLCs are potentially a strong and sensitive predictor of the risk of developing HIV-associated lymphomas. Conclusion. Based on these published findings, we suggest that free light chains have high diagnostic sensitivity, which probably enables application in laboratory diagnostics.

scholarly journals Diagnostic utility of kappa free light chains in multiple sclerosis

2019 ◽  
Vol 19 (4) ◽  
pp. 277-279 ◽  
Author(s):  
Batia Kaplan ◽  
Esther Ganelin-Cohen ◽  
Sizilia Golderman ◽  
Avi Livneh
Keyword(s):  
Multiple Sclerosis ◽  
Light Chains ◽  
Diagnostic Utility ◽  
Free Light Chains
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