scholarly journals Thin Film Biosensor for Rapid Visual Detection of Nucleic Acid Targets

1999 ◽  
Vol 45 (9) ◽  
pp. 1659-1664 ◽  
Author(s):  
Rachel M Ostroff ◽  
Deborah Hopkins ◽  
Ayla B Haeberli ◽  
Wahab Baouchi ◽  
Barry Polisky
Keyword(s):  
Thin Film ◽  
Nucleic Acid ◽  
Color Change ◽  
Film Formation ◽  
Diagnostic Testing ◽  
Visual Signal ◽  
Nucleic Acid Detection ◽  
Organic Thin Film ◽  
Allelic Discrimination ◽  
Silicon Based

Abstract Background: We have developed a silicon-based biosensor that generates a visual signal in response to nucleic acid targets. Methods: In this system, capture oligonucleotide probes are immobilized on the surface of the biosensor. Interaction of the capture probes with a complementary target and a biotinylated detector oligonucleotide allows initiation of formation of an organic thin film on the biosensor. Thin film formation is completed by enzymatic activity of peroxidase conjugated to an anti-biotin antibody. Peroxidase catalyzes deposition of an insoluble product onto the silicon surface, generating a uniform thin film. The increased thickness on the surface alters the perceived color of the biosensor through changes in the interference patterns of reflected light from the surface, causing a color change from gold to purple. Results: The biosensor results may be evaluated by direct visual inspection or quantified by ellipsometry. Results are obtained in 25 min with a detection limit of 5 pmol/L (150 amol/sample). Selectivity of the biosensor is demonstrated by discrimination of single nucleotide mismatches. Multitarget arrays are also analyzed with the thin film biosensor, and the system is capable of detecting targets from human serum and urine. Conclusions: The biosensor surface is inexpensive to produce, and the assay format is simple and rapid. The thin film biosensor is adaptable to a wide variety of nucleic acid detection applications, including rapid diagnostic testing for infectious disease panels, antibiotic resistance panels, or allelic discrimination of specific genetic markers.

scholarly journals Silicon-based Biosensors for Rapid Detection of Protein or Nucleic Acid Targets

2001 ◽  
Vol 47 (10) ◽  
pp. 1894-1900 ◽  
Author(s):  
Robert Jenison ◽  
Helen La ◽  
Ayla Haeberli ◽  
Rachel Ostroff ◽  
Barry Polisky
Keyword(s):  
Thin Film ◽  
Nucleic Acid ◽  
Interferon Γ ◽  
Wild Type ◽  
Signal To Noise ◽  
Cytokine Detection ◽  
Apparent Color ◽  
Multianalyte Detection ◽  
A Charge ◽  
Silicon Based

Abstract Background: We developed a silicon-based biosensor that generates visual, qualitative results or quantitative results for the detection of protein or nucleic acid targets in a multiplex format. Methods: Capture probes were immobilized either passively or covalently on the optically coated surface of the biosensor. Intermolecular interactions of the immobilized capture probe with specific target molecules were transduced into a molecular thin film. Thin films were generated by enzyme-catalyzed deposition in the vicinity of the surface-bound target. The increased thickness on the surface changed the apparent color of the biosensor by altering the interference pattern of reflected light. Results: Cytokine detection was achieved in a 40-min multiplex assay. Detection limits were 4 ng/L for interleukin (IL)-6, 31 ng/L for IL1-β, and 437 ng/L for interferon-γ. In multianalyte experiments, cytokines were specifically detected with signal-to-noise ratios ranging from 15 to 80. With a modified optical surface, specificity was also demonstrated in a nucleic acid array with unambiguous discrimination of single-base changes in a 15-min assay. For homozygous wild-type and homozygous mutant samples, signal-to-noise ratios of ∼100 were observed. Heterozygous samples yielded approximately equivalent signals for wild-type and mutant capture probes. Conclusions: The thin-film biosensor allows rapid, sensitive, and specific detection of protein or nucleic acid targets in an array format with results read visually or quantified with a charge-coupled device camera. This biosensor is suited for multianalyte detection in clinical diagnostic assays.

scholarly journals Versatile Method of Organic Thin Film Formation as an Approach to Optical Devices

2002 ◽  
Vol 75 (3) ◽  
pp. 111-116
Author(s):  
Toshiko MIZOKURO ◽  
Hiroyuki MOCHIZUKI ◽  
Noritaka YAMAMOTO ◽  
Takashi HIRAGA
Keyword(s):  
Thin Film ◽  
Film Formation ◽  
Optical Devices ◽  
Organic Thin Film ◽  
Thin Film Formation

scholarly journals A novel silicon based mags-biosensor for nucleic acid detection by magnetoelectronic transduction

2015 ◽  
Vol 6 ◽  
pp. 85-89 ◽  
Author(s):  
Maria Eloisa Castagna ◽  
Salvatore Petralia ◽  
Maria Grazia Amore ◽  
Emanuele Cappello ◽  
Angela Beninato ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Silicon Based

scholarly journals Rapidemic, a versatile and label-free DNAzyme-based platform for visual nucleic acid detection

2020 ◽  
Author(s):  
Marijn van den Brink ◽  
Sebastian T. Tandar ◽  
Tim A. P. van den Akker ◽  
Sinisha Jovikj ◽  
Violette Defourt ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Infectious Diseases ◽  
Detection Method ◽  
Point Of Care ◽  
Diagnostic Testing ◽  
Nucleic Acid Detection ◽  
Label Free ◽  
Strand Displacement Amplification ◽  
Sequence Detection ◽  
Accessible Point

AbstractIn the last three decades, there have been recurring outbreaks of infectious diseases, brought to light with the recent outbreak of coronavirus disease 2019 (COVID-19). Attempts to effectively contain the spread of infectious diseases have been hampered by the lack of rapidly adaptable, accurate, and accessible point-of-care diagnostic testing. In this study, we present a novel design of a label-free DNAzyme-based detection method called Rapidemic. This assay combines recombinase polymerase amplification (RPA) with linear strand-displacement amplification (LSDA) and guanine-quadruplex (GQ) DNAzyme-catalysed colour-changing reaction. The colorimetry basis of the signal readout omits the need for extensive instrumentation. Moreover, the primer-based sequence detection of RPA gives Rapidemic a potential to be rapidly adapted to target a new sequence. As a proof of concept, we developed the assay to detect isolated genomic DNA of Saccharomyces cerevisiae. The use of low-pH buffers and the optimization of the dilution rates from each preceding reaction to the next showed to be successful strategies to enable visible detection with this method. These findings demonstrate for the first time that a label-free DNAzyme-based detection method can be coupled to RPA and LSDA for nucleic acid detection.

Organic Thin Film Formation Using Asymmetric Surface-Active Viologens

Langmuir ◽  
2007 ◽  
Vol 23 (4) ◽  
pp. 1912-1916 ◽  
Author(s):  
Nabeen K. Shrestha ◽  
Hirotaka Kobayashi ◽  
Tetsuo Saji
Keyword(s):  
Thin Film ◽  
Film Formation ◽  
Organic Thin Film ◽  
Surface Active ◽  
Thin Film Formation

scholarly journals Application for Organic Thin Film Formation

1987 ◽  
Vol 57 (5) ◽  
pp. 310-317
Author(s):  
Shinzo Morita ◽  
Shuzo Hattori
Keyword(s):  
Thin Film ◽  
Film Formation ◽  
Organic Thin Film ◽  
Thin Film Formation

In situ reflectance imaging of organic thin film formation from solution deposition

2013 ◽  
Vol 114 ◽  
pp. 89-98 ◽  
Author(s):  
Jonas Bergqvist ◽  
Scott A. Mauger ◽  
Kristofer Tvingstedt ◽  
Hans Arwin ◽  
Olle Inganäs
Keyword(s):  
Thin Film ◽  
Film Formation ◽  
Organic Thin Film ◽  
Solution Deposition ◽  
Thin Film Formation

scholarly journals The Laboratory Diagnosis ofNeisseria gonorrhoeae

2005 ◽  
Vol 16 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Lai-King Ng ◽  
Irene E Martin
Keyword(s):  
Nucleic Acid ◽  
Diagnostic Testing ◽  
Laboratory Diagnosis ◽  
Detection Methods ◽  
Nucleic Acid Detection ◽  
Biochemical Tests ◽  
Culture Methods ◽  
Gene Target ◽  
Clinical Specimens ◽  
Confirmatory Tests

The present article describes the laboratory diagnosis ofNeisseria gonorrhoeaeby culturing of the organism from different types of clinical specimens followed by confirmatory tests. The success of culture methods requires good quality collection and transport of clinical specimens. The present guide describes the media requirements and cultural conditions forN gonorrhoeaegrowth and the characteristics for a presumptive identification ofN gonorrhoeae. Confirmatory tests include biochemical tests, chromogenic enzyme substrate tests, immunoassays and nucleic acid methods. Nucleic acid detection methods include either amplification-based methods or nonamplification tests, and are increasingly used in clinical laboratories where a viable culture is not possible to obtain. Nucleic acid methods can also be used to detect the presence of low numbers in a specimen. Nucleic acid detection methods need confirmation with another amplification method or gene target. Controls must be included to ensure true positive and negative results, and to rule out nucleic acid contamination. Monitoring of antimicrobial susceptibilities ofN gonorrhoeaeis important to investigate treatment failure and to evaluate the efficacy of currently recommended therapies. Many methods for the characterization ofN gonorrhoeaerequire cultures. The useful typing methods for determining strain relatedness include auxotyping, serotyping, plasmid profile analysis, DNA sequencing of theporBgene and pulsed-field gel electrophoresis. Quality assurance programs for diagnostic testing and antimicrobial susceptibility testing is reviewed.

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