scholarly journals P667 Combined therapy of perianal fistulas with autologous adipose-derived stem cells

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S548-S548
Author(s):  
A Surowiecka ◽  
M Łodyga ◽  
M Durlik ◽  
R Grażyna
Keyword(s):  
Stem Cells ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Subcutaneous Fat ◽  
Good Resolution ◽  
Adipose Derived Stem Cells ◽  
Fat Tissue ◽  
Perianal Fistulas ◽  
The Mean ◽  
Subcutaneous Fat Tissue

Abstract Background Autologous adipose-derived stem cells (ADSCs) are pluripotent medical stimulatory cells. In damaged tissues, they activate regeneration by secretion of various growth factors, anti-inflammatory cytokines and migration factors. Autologous ADSCs are harvested from subcutaneous fat tissue. Methods The aim of our study was to create a protocol of combined surgical and biologic treatment of perianal fistulas followed with transfer of autologous ADSCs into fistulas. It was a prospective study. We selected patients diagnosed with Crohn’s disease with perianal fistulas, confirmed in MRI or transanal US, with no symptoms of abscess formation. In this protocol, we started from fistulas setoning. Afterwards, two courses of biologic agents were conducted. In the next step, autologous ADSCs were harvested from subcutaneous fat tissue and transferred to inactive fistulas. We used Arthrex®system to acquire ADSCs. Further courses with biologic agents followed the ADSCs transfer. To evaluate the outcomes we used Pikarky’s Perianal Crohn’s Disease Activity Index. A good resolution was defined as fistula closure, incomplete healing as partial resolution without complete closure and fistula reactivation as no resolution. Results In a preliminary study we collected data of 10 patients. The mean volume of lipoaspirate was 30 ml. The mean amount of ADSCs transferred was 4 ml. The mean operative time was 90 min. The early observation time varied from 1 to 3 months. In all cases, these were bifurcated complex and trans-sphincteric fistulas. There were no cases of no resolution. Early results were promising with almost all cases of good resolution. However, longer observation is required. Conclusion Autologous adipose-derived stem cells in combination with surgical setoning and biological agents are a good alternative for patients with perianal manifestation of Crohn’s disease.

Subcutaneous Fat Tissue Engineering Using Autologous Adipose-Derived Stem Cells Seeded onto a Collagen Scaffold

2012 ◽  
Vol 130 (6) ◽  
pp. 1208-1217 ◽  
Author(s):  
Charlotte Lequeux ◽  
Georgette Oni ◽  
Corrine Wong ◽  
Odile Damour ◽  
Rod Rohrich ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Subcutaneous Fat ◽  
Collagen Scaffold ◽  
Adipose Derived Stem Cells ◽  
Fat Tissue ◽  
Subcutaneous Fat Tissue

scholarly journals The quality of pork ham - tissue yield depending on individual factors

2018 ◽  
Vol 34 (4) ◽  
pp. 395-404
Author(s):  
Cedomir Radovic ◽  
Marija Gogic ◽  
Nenad Parunovic ◽  
Dragan Radojkovic ◽  
Radomir Savic ◽  
...  
Keyword(s):  
Muscle Tissue ◽  
Subcutaneous Fat ◽  
Fatty Tissue ◽  
Fat Tissue ◽  
Large White ◽  
Mean Values ◽  
Four Seasons ◽  
The Mean ◽  
Subcutaneous Fat Tissue ◽  
Sire Breed

The study included the progeny of three boar-sires breeds (SL - Swedish Landrace; LW - Large White and P - Pietrain). A total of 201 progeny of both sexes (93 female and 108 male castrated animals), originating from 16 boar-sires, were tested. The study included the progeny of 10 SL boar-sires (sires nuRWer: 1, 2, 3, 7, 8, 9, 15, 16, 17 and 18), progeny of 3 LW sires (sires nuRWer: 4, 5 and 6) and 3 P boar-sires (sires nuRWer 14, 19 and 20), born in four seasons (winter, spring, summer and autumn). Studies have shown that, with an mean weight of a warm carcass side of 81.20 kg, the highest mean values for ham weight (RW; 10.456 kg), mass of intermuscular fatty tissue (RINT; 0.477 kg), ham bone (RB; 0.837 kg) and muscle tissue RMT, 7,939 kg) have progeny of the sires of Pietrain breed (P) compared to SL and LW sires. In comparison to animals sired by SL and LW boars, the progeny of P sires had less skin and subcutaneous fat tissue (RSFT) by 30 and 549 grams. Studies have shown that we have progeny of sires 7 and 9 of SL breed which have the lowest LSMean values for the yield of skin and subcutaneous fat tissue (869 and 876 g), which is below the mean for breed by 364 and 357 g. In addition, when it comes to intermuscular fatty tissue, the lowest established value was recorded in the progeny of sire no. 8 of SL breed (182 g), which is by 220 g less than the general mean and by 132 g below the mean of the sire breed. The animals originating from sires n. 19 and 20 showed the highest weight of muscle tissue (RMT) (8.489 and 8.118 kg) in the ham, which is by 2.853 and 2.482 kg more meat compared to the progeny of sire no. 5 of LW breed. The total weight of the ham and the ham muscle yield were influenced by (P <0.01 and P <0.001) sire breed, sires within the breed, gender and season of birth. A very significant (P <0.001) influence of the weight of warm carcass sides on the ham weight and tissue yield was determined.

scholarly journals Adipose-Derived Stem Cells in Crohn's Rectovaginal Fistula

2010 ◽  
Vol 2010 ◽  
pp. 1-3 ◽  
Author(s):  
D. García-Olmo ◽  
D. Herreros ◽  
P. De-La-Quintana ◽  
H. Guadalajara ◽  
J. Trébol ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Stem Cells ◽  
Clinical Trials ◽  
Mesenchymal Stem Cells ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Adipose Derived Stem Cells ◽  
Vaginal Fistula ◽  
History Of ◽  
First Time

Therapeutic options for recto-vaginal fistula in the setting of Crohn's disease are limited and many data are available in the literature. The manuscript describes the history of a patient who has been the pioneer of our Clinical Trials in treating this disease in fistulizing Crohn's disease environment. We believe it is the first time that a patient with this disease has been treated by adipose-derived stem cells in allogeneic form. The conclusion of our study with Mary is that the use of mesenchymal stem cells derived from adipose tissue is secure, either in autologous or allogeneic form. Furthermore, we have proved that if we use multi-dose and multiple applications on a patient, it does not produce any adverse effect, which confirms us the safety of using these cells in patients at least in the fistulizing Crohn's disease environment.

Safety of Allogeneic Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Complex Perianal Fistulas Not Associated With Crohn’s Disease

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Víctor Manuel Maciel Gutiérrez ◽  
Sergio Giovanni Gutiérrez Guillen ◽  
Manuel Willebaldo Centeno Flores ◽  
Jesús Alonso Valenzuela Pérez ◽  
Francisco Manuel Abarca Rendón ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Perianal Fistulas

scholarly journals DOP05 Adipose-derived stem cells from Crohn’s disease patients show antigen presenting cell-like properties

2019 ◽  
Vol 13 (Supplement_1) ◽  
pp. S030-S030
Author(s):  
C Serena ◽  
M Terrón-Puig ◽  
M Ejarque ◽  
F Algaba-Chueca ◽  
E Maymó-Masip ◽  
...  
Keyword(s):  
Stem Cells ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Adipose Derived Stem Cells ◽  
Antigen Presenting Cell ◽  
Antigen Presenting

A note on fertilization and embryo production in superovulated cattle with various levels of subcutaneous fat tissue

1990 ◽  
Vol 51 (2) ◽  
pp. 426-430 ◽  
Author(s):  
A. Bielanski ◽  
B. R. Yadav
Keyword(s):  
Body Weight ◽  
Total Dose ◽  
Abdominal Wall ◽  
Positive Relationship ◽  
Subcutaneous Fat ◽  
Corpora Lutea ◽  
Holstein Cattle ◽  
Fat Tissue ◽  
The Mean ◽  
Subcutaneous Fat Tissue

One hundred and five Holstein cattle, with a mean body weight of 668 kg, were superovulated using a total dose of 18 mg FSH. Five animals did not have corpora lutea (CL). No ova or embryos were recovered from 12 of the 100 animals which had CL. A total of 810 ova/embryos were collected from the remaining 88 animals and 432 of these were of transferable quality. The mean (range) measurements for subcutaneous fat deposition in samples taken at slaughter from the back, rump and ventral abdominal wall were 14 mm (3 to 34), 7 mm (0·7 to 33) and 7 mm (0·8 to 32), respectively. The number of transferable embryos and fertilized ova decreased as fat levels increased, but the differences between the groups were not significant (P > 0·05). There was a positive relationship between the number of CL and (a) the number of fertilized ova, and (b) the number of transferable embryos (r = 0·53, P < 0·001 and r = 0·48, P < 0·001). The correlation between the number of fertilized embryos and the number of transferable embryos was r = 0·91, P < 0·001.

scholarly journals Autologous Mesenchymal Stem Cells, Applied in a Bioabsorbable Matrix, for Treatment of Perianal Fistulas in Patients With Crohn's Disease

2017 ◽  
Vol 153 (1) ◽  
pp. 59-62.e2 ◽  
Author(s):  
Allan B. Dietz ◽  
Eric J. Dozois ◽  
Joel G. Fletcher ◽  
Greg W. Butler ◽  
Darcie Radel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Perianal Fistulas ◽  
Autologous Mesenchymal Stem Cells
Sign in / Sign up

Export Citation Format

Share Document