Adipose-Derived Stem Cells and Ceiling Culture-Derived Preadipocytes Cultured from Subcutaneous Fat Tissue Differ in Their Epigenetic Characteristics and Osteogenic Potential

Abstract Background Autologous adipose-derived stem cells (ADSCs) are pluripotent medical stimulatory cells. In damaged tissues, they activate regeneration by secretion of various growth factors, anti-inflammatory cytokines and migration factors. Autologous ADSCs are harvested from subcutaneous fat tissue. Methods The aim of our study was to create a protocol of combined surgical and biologic treatment of perianal fistulas followed with transfer of autologous ADSCs into fistulas. It was a prospective study. We selected patients diagnosed with Crohn’s disease with perianal fistulas, confirmed in MRI or transanal US, with no symptoms of abscess formation. In this protocol, we started from fistulas setoning. Afterwards, two courses of biologic agents were conducted. In the next step, autologous ADSCs were harvested from subcutaneous fat tissue and transferred to inactive fistulas. We used Arthrex®system to acquire ADSCs. Further courses with biologic agents followed the ADSCs transfer. To evaluate the outcomes we used Pikarky’s Perianal Crohn’s Disease Activity Index. A good resolution was defined as fistula closure, incomplete healing as partial resolution without complete closure and fistula reactivation as no resolution. Results In a preliminary study we collected data of 10 patients. The mean volume of lipoaspirate was 30 ml. The mean amount of ADSCs transferred was 4 ml. The mean operative time was 90 min. The early observation time varied from 1 to 3 months. In all cases, these were bifurcated complex and trans-sphincteric fistulas. There were no cases of no resolution. Early results were promising with almost all cases of good resolution. However, longer observation is required. Conclusion Autologous adipose-derived stem cells in combination with surgical setoning and biological agents are a good alternative for patients with perianal manifestation of Crohn’s disease.

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Subcutaneous Fat Tissue Engineering Using Autologous Adipose-Derived Stem Cells Seeded onto a Collagen Scaffold

Plastic & Reconstructive Surgery ◽

10.1097/prs.0b013e31826d100e ◽

2012 ◽

Vol 130 (6) ◽

pp. 1208-1217 ◽

Cited By ~ 32

Author(s):

Charlotte Lequeux ◽

Georgette Oni ◽

Corrine Wong ◽

Odile Damour ◽

Rod Rohrich ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Subcutaneous Fat ◽

Collagen Scaffold ◽

Adipose Derived Stem Cells ◽

Fat Tissue ◽

Subcutaneous Fat Tissue

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Is Fat Tissue a Suitable Source of Stem Cells for Musculoskeletal Regenerative Medicine Therapies: Literature Review

Manchester Medical Journal ◽

10.7227/mmj.0030 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Matthew James Hennessy

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Gold Standard ◽

Subcutaneous Fat ◽

Current Treatment ◽

Adipose Derived Stem Cells ◽

Joint Degeneration ◽

Fat Tissue ◽

Implantation Method ◽

Standard Production

This review found that adipose-derived stem cells are suitable for treating osteoarthritis. Besides joint replacement the current treatments do not reverse the underlining pathology, whereas this therapy replaces lost or damaged tissue and could represent a cure. Bone marrow is the traditional source of stem cells, however adipose tissue is more appropriate because of ease of harvesting, abundance, the concentration of stem cells and potential cosmetic benefits. Subcutaneous fat-derived stem cells are more effective for treating osteoarthritis when compared to visceral fat-derived. Human and animal models have shown its efficacy but with limitations. It has been less successful in older and/or patients with greater joint degeneration. Its cost-effectiveness when compared to current treatment has not been shown. A gold standard production and implantation method have yet to be developed, meaning there are no guidelines for its use. Current studies aim to address these issues, with implementation on the horizon.

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Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.35573 ◽

2015 ◽

Vol 104 (2) ◽

pp. 377-387 ◽

Cited By ~ 9

Author(s):

Ismail Rozila ◽

Pedram Azari ◽

Sha'ban Munirah ◽

Wan Kamarul Zaman Wan Safwani ◽

Seng Neon Gan ◽

...

Keyword(s):

Stem Cells ◽

Osteogenic Potential ◽

Adipose Derived Stem Cells ◽

Human Osteoblasts

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Lupus mastitis

Tijdschrift voor Geneeskunde ◽

10.47671/tvg.77.20.155 ◽

2021 ◽

Author(s):

N. WILLERS ◽

P. BERTELOOT ◽

I. WITTEVRONGHEL ◽

G. JACOMEN ◽

V. SCHELFHOUT ◽

...

Keyword(s):

Lupus Erythematosus ◽

Inflammatory Process ◽

Breast Lesion ◽

Cell Lymphoma ◽

Subcutaneous Fat ◽

Fat Tissue ◽

Lupus Panniculitis ◽

Immune Mediated ◽

History Of ◽

Subcutaneous Fat Tissue

Lupus mastitis Systemic lupus erythematosus (SLE) infrequently presents itself as lupus panniculitis. In lupus panniculitis the subcutaneous fat tissue is involved in the inflammatory process. Lupus mastitis is again a rare variant of lupus panniculitis, probably caused by an immune-mediated inflammatory process. In literature on the topic we can find 30 cases of lupus mastitis in men and women. Lupus mastitis may present like a breast lesion suspected to be malignant, clinically and radiologically. In the differential diagnosis we consider inflammatory breast cancer, subcutaneous panniculitis-like T-cell lymphoma (SPTL) and diabetes mastopathy. With a medical history of SLE and signs of inflammation of the skin above the lesion however, a diagnosis of lupus mastopathy is more presumable. Medical treatment seems more indicated in lupus mastitis, because delayed healing after surgical procedures is often reported. Even though methylprednisolone and chloroquine give clinical improvement, they need to be repeated often because of a high risk of relapse.

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Hormonal function after ovarian transposition to the abdominal subcutaneous fat tissue

International Journal of Gynecological Cancer ◽

10.1111/j.1525-1438.2006.00280.x ◽

2006 ◽

Vol 16 (1) ◽

pp. 121-124 ◽

Cited By ~ 4

Author(s):

S. NAGAO ◽

K. FUJIWARA ◽

H. ISHIKAWA ◽

T. ODA ◽

K. TANAKA ◽

...

Keyword(s):

Subcutaneous Fat ◽

Fat Tissue ◽

Ovarian Transposition ◽

Hormonal Function ◽

Subcutaneous Fat Tissue

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The Relationship Between Abdominal Subcutan Fat Tissue Thickness and HbA1c in Pregnant Women

Gynecology Obstetrics and Reproductive Medicine ◽

10.21613/gorm.2019.970 ◽

2019 ◽

pp. 1

Author(s):

Aslihan Dilara Demir ◽

Pervin Karli ◽

Durmus Ayan

Keyword(s):

Pregnant Women ◽

Subcutaneous Fat ◽

Metabolic Parameters ◽

Strong Positive Correlation ◽

Fat Tissue ◽

Tissue Thickness ◽

Positive Correlation ◽

Endocrine Organs ◽

The Relationship ◽

Subcutaneous Fat Tissue

Objective: Obesity is a very common and important health problem and it has become widespread all over the world. Fat tissue is one of the major endocrine organs. Subcutaneous adipose tissue is associated with many diseases such as coronary artery disease, metabolic syndrome, diabetes, impaired lipid profile. Pregnancy is a special condition with metabolic changes involving all systems. We aimed to investigate the relationship and correlation between abdominal fat and HbA1c because of the specific metabolic conditions in pregnancy.Study Design: This is a retrospective study. Ninety-nine (n=99) pregnant women between the ages of 20 and 40 were included in the present study. They have not any other chronic diseases. Ultrasonography was performed between 16.-28. weeks. Abdominal subcutaneous fat tissue was measured during 16-28 weeks of pregnancy. Routine biochemical parameters and HbA1c were evaluated.Results: Subcutaneous fat tissue had a positive correlation with metabolic parameters such as weight and BMI, and a negative correlation with vitamin D level. There is a positive strong correlation with HbA1c level. There was not any correlation between HbA1c and metabolic parameters like triglyceride, LDL, HDL, and cholesterol.Conclusion: There was a strong positive correlation between abdominal subcutaneous fat tissue thickness and HbA1c. However, we didn’t find any correlation between infant weight and metabolic parameters.

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The quality of pork ham - tissue yield depending on individual factors

Biotechnology in Animal Husbandry ◽

10.2298/bah1804395r ◽

2018 ◽

Vol 34 (4) ◽

pp. 395-404

Author(s):

Cedomir Radovic ◽

Marija Gogic ◽

Nenad Parunovic ◽

Dragan Radojkovic ◽

Radomir Savic ◽

...

Keyword(s):

Muscle Tissue ◽

Subcutaneous Fat ◽

Fatty Tissue ◽

Fat Tissue ◽

Large White ◽

Mean Values ◽

Four Seasons ◽

The Mean ◽

Subcutaneous Fat Tissue ◽

Sire Breed

The study included the progeny of three boar-sires breeds (SL - Swedish Landrace; LW - Large White and P - Pietrain). A total of 201 progeny of both sexes (93 female and 108 male castrated animals), originating from 16 boar-sires, were tested. The study included the progeny of 10 SL boar-sires (sires nuRWer: 1, 2, 3, 7, 8, 9, 15, 16, 17 and 18), progeny of 3 LW sires (sires nuRWer: 4, 5 and 6) and 3 P boar-sires (sires nuRWer 14, 19 and 20), born in four seasons (winter, spring, summer and autumn). Studies have shown that, with an mean weight of a warm carcass side of 81.20 kg, the highest mean values for ham weight (RW; 10.456 kg), mass of intermuscular fatty tissue (RINT; 0.477 kg), ham bone (RB; 0.837 kg) and muscle tissue RMT, 7,939 kg) have progeny of the sires of Pietrain breed (P) compared to SL and LW sires. In comparison to animals sired by SL and LW boars, the progeny of P sires had less skin and subcutaneous fat tissue (RSFT) by 30 and 549 grams. Studies have shown that we have progeny of sires 7 and 9 of SL breed which have the lowest LSMean values for the yield of skin and subcutaneous fat tissue (869 and 876 g), which is below the mean for breed by 364 and 357 g. In addition, when it comes to intermuscular fatty tissue, the lowest established value was recorded in the progeny of sire no. 8 of SL breed (182 g), which is by 220 g less than the general mean and by 132 g below the mean of the sire breed. The animals originating from sires n. 19 and 20 showed the highest weight of muscle tissue (RMT) (8.489 and 8.118 kg) in the ham, which is by 2.853 and 2.482 kg more meat compared to the progeny of sire no. 5 of LW breed. The total weight of the ham and the ham muscle yield were influenced by (P <0.01 and P <0.001) sire breed, sires within the breed, gender and season of birth. A very significant (P <0.001) influence of the weight of warm carcass sides on the ham weight and tissue yield was determined.

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