scholarly journals Crystal structure of FadR, a fatty acid-responsive transcription factor with a novel acyl coenzyme A-binding fold

2000 ◽  
Vol 19 (19) ◽  
pp. 5167-5177 ◽  
Author(s):  
D.M.F van Aalten ◽  
C.C. DiRusso ◽  
J. Knudsen ◽  
R.K. Wierenga
2011 ◽  
Vol 31 (6) ◽  
pp. 1252-1262 ◽  
Author(s):  
J. M. Ellis ◽  
S. M. Mentock ◽  
M. A. DePetrillo ◽  
T. R. Koves ◽  
S. Sen ◽  
...  

1980 ◽  
Vol 26 (8) ◽  
pp. 863-873 ◽  
Author(s):  
Anthony F. Cacciapuoti ◽  
Stephen A. Morse

The glucose-6-phosphate dehydrogenase from Neisseria gonorrhoeae was inhibited by long-chain fatty acid acyl-coenzyme A derivatives. The inhibition was increased at low concentrations of glucose 6-phosphate and was greater with the NAD-linked activity (ca. 0.05 mM inhibitor required for 50% inhibition) than with the NADP-linked activity (ca. 0.2 mM required for 50% inhibition). Bovine serum albumin and spermine could prevent the inhibition by the acyl-coenzyme A derivatives, but neither of these compounds nor high concentrations of cofactors or substrate could reverse the effect. Dilution of enzyme–inhibitor preincubation mixtures appeared to reverse the inhibition. The inhibition by stearoyl-coenzyme A was of the mixed type, and the inhibitor appeared to have a greater affinity for the free enzyme (Ki = 0.016–0.05 mM) than for enzyme bound to cofactor or substrate (Kis = 0.07–0.08 mM). Glucose-6-phosphate dehydrogenase activity was also inhibited competitively by adenosine 5′-triphosphate and was strongly regulated by adenylate energy charge values between 0.9 and 1.0. Kinetic and other characteristics of the enzyme are presented, and the possible role of glucose-6-phosphate dehydrogenase as a target for fatty acid toxicity in gonococci, mediated in the form of the acyl-coenzyme A derivatives, is discussed.


2002 ◽  
Vol 129 (4) ◽  
pp. 1700-1709 ◽  
Author(s):  
Judy A. Schnurr ◽  
Jay M. Shockey ◽  
Gert-Jan de Boer ◽  
John A. Browse

2005 ◽  
Vol 138 (1) ◽  
pp. 402-408 ◽  
Author(s):  
Thierry Tonon ◽  
Renwei Qing ◽  
David Harvey ◽  
Yi Li ◽  
Tony Robert Larson ◽  
...  

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