scholarly journals Target DNA structure plays a critical role in Tn7 transposition

2001 ◽  
Vol 20 (4) ◽  
pp. 924-932 ◽  
Author(s):  
P. N. Kuduvalli
Keyword(s):  
Critical Role ◽  
Dna Structure ◽  
Target Dna ◽  
Tn7 Transposition

scholarly journals Target DNA Structure Plays a Critical Role in RAG Transposition

PLoS Biology ◽  
2006 ◽  
Vol 4 (11) ◽  
pp. e350 ◽  
Author(s):  
Jennifer E Posey ◽  
Malgorzata J Pytlos ◽  
Richard R Sinden ◽  
David B Roth
Keyword(s):  
Critical Role ◽  
Dna Structure ◽  
Target Dna

The complex interactions of p53 with target DNA: we learn as we go

2003 ◽  
Vol 81 (3) ◽  
pp. 141-150 ◽  
Author(s):  
Ella Kim ◽  
Wolfgang Deppert
Keyword(s):  
Tumor Suppressor ◽  
Dna Binding ◽  
Target Genes ◽  
Dna Structure ◽  
Structural Features ◽  
Binding Activity ◽  
Tumor Suppressor P53 ◽  
Dna Binding Activity ◽  
Complex Interactions ◽  
Target Dna

The most import biological function of the tumor suppressor p53 is that of a sequence-specific transactivator. In response to a variety of cellular stress stimuli, p53 induces the transcription of an ever-increasing number of target genes, leading to growth arrest and repair, or to apoptosis. Long considered as a "latent" DNA binder that requires prior activation by C-terminal modification, recent data provide strong evidence that the DNA binding activity of p53 is strongly dependent on structural features within the target DNA and is latent only if the target DNA lacks a certain structural signal code. In this review we discuss evidence for complex interactions of p53 with DNA, which are strongly dependent on the dynamics of DNA structure, especially in the context of chromatin. We provide a model of how this complexity may serve to achieve selectivity of target gene regulation by p53 and how DNA structure in the context of chromatin may serve to modulate p53 functions.Key words: tumor suppressor p53, sequence-specific DNA binding, DNA conformation, chromatin, chromatin remodeling.

scholarly journals A Preferred Target DNA Structure for Retroviral Integrasein Vitro

1998 ◽  
Vol 273 (37) ◽  
pp. 24190-24195 ◽  
Author(s):  
Richard A. Katz ◽  
Kelly Gravuer ◽  
Anna Marie Skalka
Keyword(s):  
Dna Structure ◽  
Target Dna

TTE DNA–Cu NPs: enhanced fluorescence and application in a target DNA triggered dual-cycle amplification biosensor

2017 ◽  
Vol 53 (41) ◽  
pp. 5629-5632 ◽  
Author(s):  
Guangfeng Wang ◽  
Jing Wan ◽  
Xiaojun Zhang
Keyword(s):  
Dna Structure ◽  
Ultrasensitive Detection ◽  
Enhanced Fluorescence ◽  
Target Dna ◽  
Dual Cycle

A crowded TTE DNA structure for the preparation of Cu NPs with enhanced fluorescence was prepared and applied for the ultrasensitive detection of target DNA.

scholarly journals Critical role of DNA unwinding and Cas9 conformational changes in modelling off-target effects

2021 ◽  
Author(s):  
Aset Khakimzhan ◽  
Vincent Noireaux
Keyword(s):  
Conformational Changes ◽  
Critical Role ◽  
Dna Unwinding ◽  
Ode Model ◽  
Guide Rna ◽  
Target Dna ◽  
Binding Models ◽  
Linear Ode ◽  
Target Effects

AbstractCRISPR-Cas9 off-target effects interfere with the ability to accurately perform genetic edits. To predict off-target effects CRISPR-Cas9 researchers perform high throughput guide RNA mismatch and bulge experiments and then use the data to fit thermodynamic binding models. While impactful from an engineering perspective such models are not based on the experimentally observed target interrogation process and thus incorrectly measure the energetic effects mismatches have on the system. In this work we convert an experimentally deduced qualitive model of target interrogation to a linear ODE model and demonstrate that the mismatch tolerance patterns observed in experiments do not need to be caused by differences in energetic penalties of mismatches but rather are emergent effects of the timing and coordination of target DNA unwinding and Cas9 conformational changes.

Georadar survey to explore a supposed ejecta layer around the Maâdna crater (Talemzane, Algeria)

2020 ◽  
Author(s):  
Lamine Hamai ◽  
Atmane Lamali ◽  
Abdelkrim Yelles-chaouche ◽  
Abdeslam Abtout ◽  
Abdelmadjid Nadjemi ◽  
...  
Keyword(s):  
Critical Role ◽  
Dna Structure ◽  
Structural Context ◽  
Geophysical Surveys ◽  
The Future ◽  
Survey Results ◽  
Impact Origin

<p>Geophysics continues to play a critical role in the future discovery of terrestrial impact structures. While the signatures within these structures may not be unique, the application of geophysics can effectively characterize them, even when they are deeply eroded or completely buried underground. In the case of Maâdna crater (33°19' N, 4°19' E), among new performed geophysical surveys, a GPR technique has been especially used to explore a supposed ejecta layer. However, GPR survey results allowed the confirmation of nonexistence of such as melting materials at Maâdna crater. Nevertheless, our different scans were interpretative against the structural context of the Maâdna structure. Indeed, most of the analyzed profiles allowed us recognizing the typical deformation effects at this structure, which can also generally be encountered at any crater-like structured site. Consequently, in view to this new resulting GPR data, even we do not definitely reject an impact origin, we are still pleading for other caratering scenarios for this structure.</p>

Dexamethasone Effect on Embryonic Chick Respiratory Epithelium

1982 ◽  
Vol 40 ◽  
pp. 248-249
Author(s):  
M.R. Richter ◽  
R.V. Blystone
Keyword(s):  
Lung Development ◽  
Critical Role ◽  
Respiratory Epithelium ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
White Leghorn ◽  
Lung Maturation ◽  
Synthetic Analogs ◽  
Lung Physiology ◽  
Avian Lung

Dexamethasone and other synthetic analogs of corticosteroids have been employed clinically as enhancers of lung development. The mechanism(s) by which this steroid induction of later lung maturation operates is not clear. This study reports the effect on lung epithelia of dexamethasone administered at different intervals during development. White Leghorn chick embryos were used so as to remove possible maternal and placental influences on the exogenously applied steroid. Avian lung architecture does vary from mammals; however, respiratory surfactant produced by the lung epithelia serves an equally critical role in avian lung physiology.

An atomic resolution study of a carbide phase in platinum

1992 ◽  
Vol 50 (1) ◽  
pp. 20-21
Author(s):  
M.J. Witcomb ◽  
M.A. O'Keefe ◽  
CJ. Echer ◽  
C. Nelson ◽  
J.H. Turner ◽  
...  
Keyword(s):  
Solid Solution ◽  
Carbon Atom ◽  
Carbide Phase ◽  
Structural Changes ◽  
Critical Role ◽  
Alloy System ◽  
Atomic Resolution ◽  
Excess Carbon ◽  
Interstitial Carbon ◽  
Resolution Study

Under normal circumstances, Pt dissolves only a very small amount of interstitial carbon in solid solution. Even so, an appropriate quench/age treatment leads to the formation of stable Pt2C {100} plate precipitates. Excess (quenched-in) vacancies play a critical role in the process by accommodating the volume and structural changes that accompany the transformation. This alloy system exhibits other interesting properties. Due to a large vacancy/carbon atom binding energy, Pt can absorb excess carbon at high temperatures in a carburizing atmosphere. In regions rich in carbon and vacancies, another carbide phase, Pt7C which undergoes an order-disorder reaction was formed. The present study of Pt carburized at 1160°C and aged at 515°C shows that other carbides in the PtxC series can be produced.

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