Maize (Zea mays L. Sp.) varieties significantly influence bacterial and fungal community in bulk soil, rhizosphere soil and phyllosphere

ABSTRACT The plant–microbe interaction can affect ecosystem function, and many studies have demonstrated that plant species influence relevant microorganisms. In this study, microbial communities in bulk soil, rhizosphere soil and phyllosphere from different maize varieties were investigated using high-throughput sequencing method. Results demonstrated that cultivar Gaoneng 1 (G1) showed higher bacterial diversity in soil (both bulk and rhizosphere soils) and lower bacterial diversity in the phyllosphere, while cultivar Gaoneng 2 (G2) had lower fungal diversity in both the soil and phyllosphere compare to the other cultivars. The bacterial community structure of soils among the three varieties was significantly different; however, no significant differences were found in the soil fungal community and phyllosphere bacterial and fungal community. The soil networks from cultivar G1 and phyllosphere networks from cultivar Zhengdan (ZD) have the highest complexity in contrast to the other two cultivars. In conclusion, the bacterial community structure in bulk soil of different cultivars was significantly different, so do the co-occurrence ecological networks of phyllosphere bacterial community. This study comprehensively analyzed the microbial community among different maize cultivars and could be useful for guiding practices, such as evaluation of new plant cultivars and quality predictions of these varieties at the microbial level.

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Earthworm activity optimized the rhizosphere bacterial community structure and further alleviated the yield loss in continuous cropping lily (Lilium lancifolium Thunb.)

Scientific Reports ◽

10.1038/s41598-021-99597-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yaoxiong Lu ◽

Peng Gao ◽

Yunsheng Wang ◽

Wei Li ◽

Xinwei Cui ◽

...

Keyword(s):

Community Structure ◽

Microbial Community ◽

Bacterial Community ◽

Soil Microbial Community ◽

Bacterial Community Structure ◽

Rhizosphere Soil ◽

The Other ◽

Continuous Cropping ◽

Soil Microbial ◽

Rhizosphere Bacterial Community

AbstractThe soil microbial community plays a vital role in the biogeochemical cycles of bioelements and maintaining healthy soil conditions in agricultural ecosystems. However, how the soil microbial community responds to mitigation measures for continuous cropping obstacles remains largely unknown. Here we examined the impact of quicklime (QL), chemical fungicide (CF), inoculation with earthworm (IE), and a biocontrol agent (BA) on the soil microbial community structure, and the effects toward alleviating crop yield decline in lily. High-throughput sequencing of the 16S rRNA gene from the lily rhizosphere after 3 years of continuous cropping was performed using the Illumina MiSeq platform. The results showed that Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, Chloroflexi and Gemmatimonadetes were the dominant bacterial phyla, with a total relative abundance of 86.15–91.59%. On the other hand, Betaproteobacteriales, Rhizobiales, Myxococcales, Gemmatimonadales, Xanthomonadales, and Micropepsales were the dominant orders with a relative abundance of 28.23–37.89%. The hydrogen ion concentration (pH) and available phosphorus (AP) were the key factors affecting the structure and diversity of the bacterial community. The yield of continuous cropping lily with using similar treatments decreased yearly for the leaf blight, but that of IE was significantly (p < 0.05) higher than with the other treatments in the same year, which were 17.9%, 18.54%, and 15.69% higher than that of blank control (CK) over 3 years. In addition, IE significantly (p < 0.05) increased organic matter (OM), available nitrogen (AN), AP, and available potassium (AK) content in the lily rhizosphere soil, optimized the structure and diversity of the rhizosphere bacterial community, and increased the abundance of several beneficial bacterial taxa, including Rhizobiales, Myxococcales, Streptomycetales and Pseudomonadales. Therefore, enriching the number of earthworms in fields could effectively optimize the bacterial community structure of the lily rhizosphere soil, promote the circulation and release in soil nutrients and consequently alleviate the loss of continuous cropping lily yield.

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Effects of heavy metals on bacterial community structure in the rhizosphere of Salsola collina and bulk soil in the Jinchuan mining area

Geomicrobiology Journal ◽

10.1080/01490451.2021.1914784 ◽

2021 ◽

pp. 1-11

Author(s):

Tian-Peng Gao ◽

Zi-Dong Wan ◽

Xiao-Xiao Liu ◽

Jing-Wen Fu ◽

Guo-Hua Chang ◽

...

Keyword(s):

Heavy Metals ◽

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

Mining Area ◽

Bulk Soil

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Characterization of Bacterial Community Structure in Rhizosphere Soil of Grain Legumes

Microbial Ecology ◽

10.1007/s00248-004-0041-7 ◽

2005 ◽

Vol 49 (3) ◽

pp. 407-415 ◽

Cited By ~ 61

Author(s):

S. Sharma ◽

M.K. Aneja ◽

J. Mayer ◽

J.C. Munch ◽

M. Schloter

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

Rhizosphere Soil ◽

Grain Legumes

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Difference in Rhizosphere Soil Bacterial Community was among Six Cinnamomum camphora Chemotypes

10.20944/preprints202111.0158.v1 ◽

2021 ◽

Author(s):

Zufei Xiao ◽

Beihong Zhang ◽

Yangbao Wang ◽

Zhinong Jin ◽

Feng Li ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Communities ◽

Bacterial Community Structure ◽

Rhizosphere Soil ◽

Soil Nutrient ◽

Soil Bacterial Community ◽

Cinnamomum Camphora ◽

Close Relationship ◽

Soil Bacterial

Abstract: Plant types and soil bacterial communities had a close relationship, understanding the profound association between them contributes to better learn bacterial ecological function for plant growth. In this study, rhizosphere soil of six different chemotype Cinnamomum camphora trees were collected, including C. bodinieri var. citralifera, [C. camphora (Linn.) Presl], camphora-type, cineole-type, linalool-type and isoborneol-type. Soil properties content and bacterial communities were analyzed. Two chemotype C. camphora, including [C. camphora (Linn.) Presl] and linalool-type, shaped similar bacterial community structure, decreased Firmcutes relative abundance. richness estimators (Chao1 index and Ace index) of [C. camphora (Linn.) Presl] were decreased compared with the others. Furthermore, soil bacterial community structure was also similar among bodinieri var. citralifera, camphora-type, cineole-type and isoborneol-type. Hence, different chemotype C. camphora altered soil nutrient and shaped rhizosphere bacterial communities.

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Variation in Bacterial Community Structure in Rhizosphere and Bulk Soils of Different Halophytes in the Yellow River Delta

Frontiers in Ecology and Evolution ◽

10.3389/fevo.2021.816918 ◽

2022 ◽

Vol 9 ◽

Author(s):

Yinghan Zhao ◽

Tian Li ◽

Pengshuai Shao ◽

Jingkuan Sun ◽

Wenjing Xu ◽

...

Keyword(s):

Bacterial Community ◽

Bacterial Community Structure ◽

Rhizosphere Soil ◽

Yellow River ◽

Bacterial Community Composition ◽

Yellow River Delta ◽

Bulk Soil ◽

The Yellow River ◽

The Yellow River Delta ◽

Α Diversity

Soil microorganisms play the important role in driving biogeochemical cycles. However, it is still unclear on soil microbial community characteristics and microbial driving mechanism in rhizosphere and bulk soils of different halophyte species. In this study, we analyzed bacterial communities in the rhizosphere and bulk soils of three typical halophytes in the Yellow River Delta, i.e., Phragmites communis, Suaeda salsa, and Aeluropus sinensis, by high-throughput sequencing. The contents of total carbon, total nitrogen, and available phosphorus in rhizosphere soils of the three halophytes were significantly higher than those in bulk soils, which suggested a nutrient enrichment effect of the rhizosphere. Rhizosphere soil bacterial α-diversity of P. communis was higher than that in bulk soil, whereas bacterial α-diversity in rhizosphere soil of S. salsa and A. sinensis was lower than those in bulk soil. The dominant bacterial phyla were Proteobacteria, Actinobacteria, Chloroflexi, and Bacteroidetes, which accounted for 31, 20.5, 16.3, and 10.3%, respectively. LDA effect size (LEfSe) analysis showed that the bacterial species with significant differences in expression abundance was obviously different in the rhizosphere and bulk soil of three halophytes. The principal component analysis (PCoA) showed that bacterial community composition was greatly different between rhizosphere and bulk soils of P. communis and S. salsa, while no difference in A. sinensis. Changed bacterial community composition was mainly ascribed to salinity in rhizosphere and bulk soils. Additionally, salinity was positively correlated with Bacteroidetes and negatively correlated with Actinobacteria and Acidobacteria. Our study clarified the variation in bacterial community structure between rhizosphere and bulk soils with soil physicochemical properties, which proved a biological reference to indicate the characteristics of saline and alkaline land.

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Analysis of Microbial Community Structure Around Roots of Stipa Grandis

10.21203/rs.3.rs-500898/v1 ◽

2021 ◽

Author(s):

Ai-Min Zhu ◽

Guo-Dong Han ◽

Hai-Li Liu ◽

Yue-Hua Wang

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

Rhizosphere Soil ◽

Root Zone ◽

Diversity Index ◽

Alpha Diversity ◽

Microbial Structure ◽

Root Epidermis ◽

16S Rna

Abstract The root zone microbial structure is particularly complex for plants with rhizosheaths, which may play an important role in the future agricultural sustainable development. However, one of the important reasons for restricting our study of rhizosheath microbial structure is that there is no definite method for rhizosheath separation. The aim of this study was to explore the isolation methods of rhizosheath and the diversity and functional characteristics of microorganisms around the rhizosphere. In this study, we isolated the rhizosheath of Stipa grandis, a dominant species in desert steppe, and the microorganisms in the roots, root epidermis, rhizosheath, rhizosphere soil were extracted and sequenced by 16s RNA and ITS. The bacterial alpha diversity index was in the order rhizosphere soil > rhizosheath > root epidermis > endophytic, and the fungal alpha diversity index was rhizosphere soil and rhizosheath > root epidermis and endophytic. There were significant differences in bacterial community structure between the root epidermis and endophytic, rhizosheath, rhizosphere soil, and the sum of relative abundance of the dominant bacterial populations Actinobacteria and Proteobacteria was 73.9% in the root epidermis. Different from bacterial community structure, the community structure of root epidermis fungi was similar to endophytic, but significantly different from rhizosheath and rhizosphere soil. We suggest that the root epidermis can act as the interface between the host plant root and the external soil environment. This study will provide theoretical and technical guidance for the isolation of plant rhizosheath and the study of microorganisms in it.

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