The Gossypium stocksii genome as a novel resource for cotton improvement

Abstract Cotton is an important textile crop whose gains in production over the last century have been challenged by various diseases. Because many modern cultivars are susceptible to several pests and pathogens, breeding efforts have included attempts to introgress wild, naturally resistant germplasm into elite lines. Gossypium stocksii is a wild cotton species native to Africa, which is part of a clade of vastly understudied species. Most of what is known about this species comes from pest resistance surveys and/or breeding efforts, which suggests that G. stocksii could be a valuable reservoir of natural pest resistance. Here we present a high-quality de novo genome sequence for G. stocksii. We compare the G. stocksii genome with resequencing data from a closely related, understudied species (G. somalense) to generate insight into the relatedness of these cotton species. Finally, we discuss the utility of the G. stocksii genome for understanding pest resistance in cotton, particularly resistance to cotton leaf curl virus.

Download Full-text

The Gossypium stocksii genome as a novel resource for cotton improvement

10.1101/2021.02.23.432605 ◽

2021 ◽

Author(s):

Corrinne E. Grover ◽

Daojun Yuan ◽

Mark A. Arick ◽

Emma R. Miller ◽

Guanjing Hu ◽

...

Keyword(s):

Genome Sequence ◽

De Novo ◽

Pest Resistance ◽

Cotton Leaf ◽

High Quality ◽

Cotton Leaf Curl Virus ◽

Cotton Species ◽

Wild Cotton ◽

Leaf Curl Virus ◽

Insight Into

AbstractCotton is an important textile crop whose gains in production over the last century have been challenged by various diseases. Because many modern cultivars are susceptible to several pests and pathogens, breeding efforts have included attempts to introgress wild, naturally resistant germplasm into elite lines. Gossypium stocksii is a wild cotton species native to Africa, which is part of a clade of vastly understudied species. Most of what is known about this species comes from pest resistance surveys and/or breeding efforts, which suggests that G. stocksii could be a valuable reservoir of natural pest resistance. Here we present a high-quality de novo genome sequence for G. stocksii. We compare the G. stocksii genome with resequencing data from a closely related, understudied species (G. somalense) to generate insight into the relatedness of these cotton species. Finally, we discuss the utility of the G. stocksii genome for understanding pest resistance in cotton, particularly resistance to cotton leaf curl virus.

Download Full-text

Genomic Characterization Provides an Insight into the Pathogenicity of the Poplar Canker Bacterium Lonsdalea populi

Genes ◽

10.3390/genes12020246 ◽

2021 ◽

Vol 12 (2) ◽

pp. 246

Author(s):

Xiaomeng Chen ◽

Rui Li ◽

Yonglin Wang ◽

Aining Li

Keyword(s):

Genome Sequence ◽

Extracellular Enzymes ◽

De Novo ◽

Whole Genome Sequence ◽

Hybrid Poplars ◽

A Genome ◽

Conserved Genes ◽

Genomic Characterization ◽

Molecular Bases ◽

Insight Into

An emerging poplar canker caused by the gram-negative bacterium, Lonsdalea populi, has led to high mortality of hybrid poplars Populus × euramericana in China and Europe. The molecular bases of pathogenicity and bark adaptation of L. populi have become a focus of recent research. This study revealed the whole genome sequence and identified putative virulence factors of L. populi. A high-quality L. populi genome sequence was assembled de novo, with a genome size of 3,859,707 bp, containing approximately 3434 genes and 107 RNAs (75 tRNA, 22 rRNA, and 10 ncRNA). The L. populi genome contained 380 virulence-associated genes, mainly encoding for adhesion, extracellular enzymes, secretory systems, and two-component transduction systems. The genome had 110 carbohydrate-active enzyme (CAZy)-coding genes and putative secreted proteins. The antibiotic-resistance database annotation listed that L. populi was resistant to penicillin, fluoroquinolone, and kasugamycin. Analysis of comparative genomics found that L. populi exhibited the highest homology with the L. britannica genome and L. populi encompassed 1905 specific genes, 1769 dispensable genes, and 1381 conserved genes, suggesting high evolutionary diversity and genomic plasticity. Moreover, the pan genome analysis revealed that the N-5-1 genome is an open genome. These findings provide important resources for understanding the molecular basis of the pathogenicity and biology of L. populi and the poplar-bacterium interaction.

Download Full-text

Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2014.11.013 ◽

2016 ◽

Vol 23 (3) ◽

pp. 358-362 ◽

Cited By ~ 9

Author(s):

Sayed Sartaj Sohrab ◽

Mohammad A. Kamal ◽

Abdul Ilah ◽

Azamal Husen ◽

P.S. Bhattacharya ◽

...

Keyword(s):

Transgenic Cotton ◽

Cotton Leaf ◽

Cotton Leaf Curl Virus ◽

Leaf Curl Virus ◽

Leaf Curl

Download Full-text

High density cotton population in late sowing improves productivity and tolerance to cotton leaf curl virus under semi-arid subtropical conditions

Journal of Plant Diseases and Protection ◽

10.1007/s41348-021-00442-1 ◽

2021 ◽

Author(s):

Muhammad Iqbal ◽

Mueen Alam Khan ◽

Sami Ul-Allah

Keyword(s):

High Density ◽

Cotton Leaf ◽

Cotton Leaf Curl Virus ◽

Leaf Curl Virus ◽

Semi Arid ◽

Late Sowing ◽

Leaf Curl

Download Full-text

Exploration of Cotton Leaf Curl Virus (CLCuV) resistance genes and their screening in Gossypium arboreum by targeting resistance gene analogues

AoB Plants ◽

10.1093/aobpla/ply067 ◽

2018 ◽

Author(s):

Rakhshanda Mushtaq ◽

Khurram Shahzad ◽

Shahid Mansoor ◽

Zahid Hussain Shah ◽

Hameed Alsamadany ◽

...

Keyword(s):

Resistance Gene ◽

Resistance Genes ◽

Cotton Leaf ◽

Gossypium Arboreum ◽

Cotton Leaf Curl Virus ◽

Leaf Curl Virus ◽

Leaf Curl

Download Full-text

Widespread Occurrence of Cotton leaf curl virus on Radish in Pakistan

Plant Disease ◽

10.1094/pdis.2000.84.7.809b ◽

2000 ◽

Vol 84 (7) ◽

pp. 809-809 ◽

Cited By ~ 7

Author(s):

S. Mansoor ◽

S. Mukhtar ◽

M. Hussain ◽

I. Amin ◽

Y. Zafar ◽

...

Keyword(s):

Host Range ◽

Full Length ◽

Cotton Leaf ◽

Degenerate Primers ◽

Nylon Membrane ◽

Punjab Province ◽

Cotton Leaf Curl Virus ◽

Kitchen Gardens ◽

Leaf Curl Virus ◽

Leaf Curl

The current epidemic of cotton leaf curl disease (CLCuD) in Pakistan started in 1988 with the natural host range limited to a few plant species in the family Malvaceae. However, we have observed expansion in the host range of the virus, and several non-Malvaceous plants were found to be infected with the virus. Characteristic symptoms of CLCuD such as leaf curl and enations have been observed on radish plants, primarily in kitchen gardens. However, in 1999, levels of infection of 10 to 90% were observed both in commercial fields and kitchen gardens in the Punjab province of Pakistan. Both symptomatic and nonsymptomatic samples were collected from five different locations. Total DNA was isolated, dot-blotted on nylon membrane, and a full-length clone corresponding to DNA A of cotton leaf curl virus was labeled with 32P dCTP and used as a probe for the detection of a begomovirus. Strong signals were observed in symptomatic plants while no signals were observed in nonsymptomatic plants. Infection with a begomovirus was further confirmed by polymerase chain reaction (PCR) using degenerate primers for DNA A (1). Primers specific for the two distinct begomoviruses associated with CLCuD were also used in PCR reactions (2), and products of the expected size were obtained from all symptomatic samples, confirming infection with begomoviruses similar to those associated with CLCuD. A full-length probe of a nanovirus-like molecule associated with cotton leaf disease (3), called DNA 1 was labeled with 32P dCTP and detected the virus only in symptomatic plants. Similarly, primers specific for DNA 1 (3) amplified a product of expected size when used in PCR. On the basis of symptomatology and the detection of specific viral components associated with the disease, we confirmed that radish plants are infected with Cotton leaf curl virus (CLCuV). Since radish is a short duration crop, infection of CLCuV in radish may not serve as a direct source of infection for the next cotton crop. However, it is a potential threat to tomato crops which overlap with radish in the Punjab province. The detection of CLCuD in radish is another example of the mobilization of begomoviruses to previously unknown hosts. References: (1) M. R. Rojas et al. Plant Dis. 77:340, 1993. (2) S. Mansoor et al. Pak. J. Bot. 31:115, 1999. (3) Mansoor et al. Virology 259:190, 1999.

Download Full-text

Estimation of Genetic Effects Controlling Different Plant Traits in Cotton (Gossypium Hirsutum L.) Under Clcuv Epidemic Condition

Cercetari Agronomice in Moldova ◽

10.1515/cerce-2017-0004 ◽

2017 ◽

Vol 50 (1) ◽

pp. 47-56 ◽

Cited By ~ 1

Author(s):

A.M. Khan ◽

S. Fiaz ◽

I. Bashir ◽

S. Ali ◽

M. Afzal ◽

...

Keyword(s):

Plant Traits ◽

Fiber Strength ◽

Block Design ◽

Cotton Leaf ◽

Cotton Leaf Curl Virus ◽

Fiber Fineness ◽

Cotton Genotypes ◽

Seed Index ◽

Leaf Curl Virus ◽

Additive Genes

Abstract Nine cotton genotypes, comprised of four non Bt males (CIM-1100, CIM-506, FH-942 and FH-900), five Bt female genotypes (FH-113, FH-114, MNH- 886, AA-703 and IR-3701) and their 20 crosses were screened in order to evaluate their response to Cotton leaf curl virus symptoms through epidemiology in the field and greenhouse. The 20 crosses among their nine parents were sown in two replications under randomized complete block design, during 2013 and 2014. The mean squares were significant for all traits indicating that both additive and non-additive genes control the characters, but non-additive genes were more important because, variance of dominant genes were higher than additive genes. In our test, FH-900 showed the best performance against CLCuV, number of lobes per boll and seed yield. The CIM- 1100 genotype performed well in boll weight, fiber strength and fiber fineness, whereas CIM-506 was good for plant height, number of sympodial branches and ginning. Maximum boll number, seed index and fiber length were shown by FH-942. Among lines, MNH886, FH-113, IR-3701 and FH-114 exhibited the best general combination for many traits. Hence, parents were preferred for hybridization program to improve the majority of characters. Hybrids FH-113 × FH-942, MNH-886 × CIM-1100, MNH-886 × FH-942, IR-3701 × CIM-506, AA-703 × CIM-1100, FH-114 × FH-942, FH-114 × CIM-1100 and MNH-886 × FH- 900 was best in specific combinations for different traits, especially against CLCuV. The results indicated that to increase resistance against CLCuV, hybrids should be exploited to develop CLCuV resistance.

Download Full-text