scholarly journals Molecular characterization of the mitochondrial DNA of a new stopper mutant ER-3 of Neurospora crassa.

Genetics ◽  
1988 ◽  
Vol 120 (4) ◽  
pp. 935-945
Author(s):  
A Almasan ◽  
N C Mishra
Keyword(s):  
Mitochondrial Dna ◽  
Neurospora Crassa ◽  
Molecular Level ◽  
Wild Type ◽  
Type Molecule ◽  
Nd5 Gene ◽  
Nucleotide Repeats ◽  
Two Populations ◽  
Mitochondrial Chromosome

Abstract An ethidium bromide-induced stopper mutant of Neurospora crassa is characterized at the molecular level. The mutant has two populations of mitochondrial DNA: a defective predominant mutant molecule and a basal level of the wild-type molecule. The aberrant DNA resulted after a 25-kbp deletion from the wild-type mitochondrial chromosome, which included major genes such as cytb, co1 and oli2. The deletion endpoints are located in the second intron of the ND5 gene, and in a sequence 250 nucleotides upstream of the co2 gene. The recombination has taken place between two nine nucleotide repeats CCCCGCCCC, one of which is close to a PstI palindrome at its 5' end. Thus the mutant ER-3 differs from all the other stopper mutants described previously in the extent and location of the deletions in the mtDNA.

scholarly journals Characterization of Neurospora crassa cyclic AMP phosphodiesterase activated by calmodulin

1985 ◽  
Vol 232 (2) ◽  
pp. 425-430 ◽  
Author(s):  
M T Téllez-Iñón ◽  
R M Ulloa ◽  
G C Glikin ◽  
H N Torres
Keyword(s):  
Cyclic Amp ◽  
Neurospora Crassa ◽  
Cyclic Nucleotide ◽  
Neuroleptic Drugs ◽  
Wild Type ◽  
Cyclic Amp Phosphodiesterase ◽  
Aerial Hyphae

Activation of cyclic AMP phosphodiesterase I by brain or Neurospora calmodulin was studied. The stimulation required micromolar concentrations of Ca2+, and it was observed at cyclic AMP concentrations between 0.1 and 500 microM. Activation was blocked by EDTA and some neuroleptic drugs such as chlorpromazine and fluphenazine. These drugs inhibit the elongation of N. crassa wild-type aerial hyphae. These results reinforce the evidence towards the recognition of Ca2+-calmodulin as one of the systems controlling cyclic nucleotide concentrations in Neurospora.

Chromosomal and Mitochondrial DNA Characterization of a Population of Dwarf Cisco (Coregonus artedii) in Minnesota

1990 ◽  
Vol 47 (8) ◽  
pp. 1562-1569 ◽  
Author(s):  
Barbara A. Shields ◽  
Kevin S. Guise ◽  
James C. Underhill
Keyword(s):  
Mitochondrial Dna ◽  
Chromosome Complement ◽  
Dna Profile ◽  
Normal Populations ◽  
Study Population ◽  
Coregonus Artedii ◽  
Mtdna Diversity ◽  
Two Populations ◽  
Restriction Site Polymorphisms

This study compared the chromosome complement and mitochondrial DNA profile of a population of dwarf cisco in Ten Mile Lake, Cass County, Minnesota, to two populations of normal-sized Coregonus artedii in order to determine if the dwarf fish were conspecific with C. artedii. Chromosomal analysis revealed a diploid chromosome number (2n = 80) the same as reported for C. artedii. Mitochondrial DNA (mtDNA) analysis revealed unexpected levels of intrapopulational diversity in each study population, with the dwarf fish displaying the highest level of mtDNA diversity. Heteroplasmy, restriction site polymorphisms, and three size variants were documented. Each population was characterized by a unique profile of mtDNA clonal types, yet interpopulational comparisons revealed more similarities between the dwarf fish in Ten Mile Lake and the two normal populations than between the populations of normal-sized fish. Based on these data, the dwarf fish of Ten Mile Lake should be considered conspecific with Coregonus artedii.

scholarly journals REGULATION OF PHOSPHATE METABOLISM IN NEUROSPORA CRASSA. CHARACTERIZATION OF REGULATORY MUTANTS

Genetics ◽  
1973 ◽  
Vol 75 (1) ◽  
pp. 61-73
Author(s):  
John F Lehman ◽  
Mary K Gleason ◽  
Sandra K Ahlgren ◽  
Robert L Metzenberg
Keyword(s):  
Alkaline Phosphatase ◽  
Neurospora Crassa ◽  
Control Systems ◽  
Double Mutant ◽  
Phosphorus Metabolism ◽  
Phosphate Metabolism ◽  
Wild Type Allele ◽  
Wild Type ◽  
High Affinity

ABSTRACT A mutant of Neurospora crassa, called UW-6, differs from wild type in being partially constitutive for synthesis of a species of alkaline phosphatase, and also for a species of phosphate permease that has a high affinity for phosphate at high pH. UW-6 is possibly allelic with a mutant called nuc-2 that was previously isolated by Ishikawa. nuc-2 has the converse phenotype, in that it cannot be derepressed for either of these two activities. UW-6 is co-dominant with its wild-type allele in heterokaryons and in partial diploids. An unlinked mutant, nuc-1, is like nuc-2 in that it fails to make the alkaline phosphatase or the permease referred to above. nuc-1 is epistatic to UW-6 in the double mutant. The control of phosphorus metabolism is discussed, and is compared with some other control systems in filamentous fungi.

scholarly journals A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA

1971 ◽  
Vol 51 (1) ◽  
pp. 249-264 ◽  
Author(s):  
David D. Wood ◽  
David J. L. Luck
Keyword(s):  
Ionic Strength ◽  
Neurospora Crassa ◽  
Soluble Form ◽  
Wild Type ◽  
Immunological Properties ◽  
End To End ◽  
Single Polypeptide ◽  
The One

A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine. This paracrystal has been isolated and partially characterized. It appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the pH and ionic strength. When aggregated, the polypeptide forms oligomers which are arranged end-to-end into fibers. During the characterization of the polypeptide, it was found that the polypeptide's electrophoretic and immunological properties could be used as assays. Using these methods it was found that the polypeptide normally accumulates in a soluble form in the cytoplasm of wild-type Neurospora at the end of the log-phase of growth.

A “Stopper” mutant of Neurospora crassa containing two populations of aberrant mitochondrial DNA

1981 ◽  
Vol 3 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Hans de Vries ◽  
Jenny C. de Jonge ◽  
Peter van't Sant ◽  
Etienne Agsteribbe ◽  
Annika Arnberg
Keyword(s):  
Mitochondrial Dna ◽  
Neurospora Crassa ◽  
Two Populations

scholarly journals RNA Multimerisation in the DNA Packaging Motor of Bacteriophage φ29

2005 ◽  
Vol 6 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Jonathan P. A. Wood ◽  
Stephanie A. Capaldi ◽  
Mark A. Robinson ◽  
Andrew J. Baron ◽  
Nicola J. Stonehouse
Keyword(s):  
Analytical Ultracentrifugation ◽  
Molecular Level ◽  
Viral Assembly ◽  
Partial Specific Volume ◽  
Wild Type ◽  
Rna Molecules ◽  
Dna Packaging Motor ◽  
Self Association

The use of bacteriophages as experimental tools allows the investigation of interactions between components at the molecular level that are often not possible in more complex virus systems. The bacteriophage φ29 acts as a molecular machine to package its own genomic DNA during viral assembly. Self-associating RNA molecules, called pRNA, have an essential role in the function of this machine. This paper reports the characterization of this self-association (which leads to multimerisation of wild-type and truncated variant pRNAs) by analytical ultracentrifugation (including determination of the partial specific volume of the pRNA), together with an investigation into the domains of the molecule important for multimerisation by the use of complementary DNA probes.

Characterization of mat A-2, mat A-3 and ΔmatA Mating-Type Mutants of Neurospora crassa

Genetics ◽  
1998 ◽  
Vol 148 (3) ◽  
pp. 1069-1079 ◽  
Author(s):  
Adlane V-B Ferreira ◽  
Zhiqiang An ◽  
Robert L Metzenberg ◽  
N Louise Glass
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Sexual Development ◽  
Vegetative Growth ◽  
Double Mutant ◽  
Sexual Cycle ◽  
Wild Type ◽  
Type Locus ◽  
Isolation And Characterization

AbstractThe mating-type locus of Neurospora crassa regulates mating identity and entry into the sexual cycle. The mat A idiomorph encodes three genes, mat A-1, mat A-2, and mat A-3. Mutations in mat A-1 result in strains that have lost mating identity and vegetative incompatibility with mat a strains. A strain containing mutations in both mat A-2 and mat A-3 is able to mate, but forms few ascospores. In this study, we describe the isolation and characterization of a mutant deleted for mat (ΔmatA), as well as mutants in either mat A-2 or mat A-3. The ΔmatA strain is morphologically wild type during vegetative growth, but it is sterile and heterokaryon compatible with both mat A and mat a strains. The mat A-2 and mat A-3 mutants are also normal during vegetative growth, mate as a mat A strain, and produce abundant biparental asci in crosses with mat a, and are thus indistinguishable from a wild-type mat A strain. These data and the fact that the mat A-2 mat A-3 double mutant makes few asci with ascospores indicate that MAT A-2 and MAT A-3 are redundant and may function in the same pathway. Analysis of the expression of two genes (sdv-1 and sdv-4) in the various mat mutants suggests that the mat A polypeptides function in concert to regulate the expression of some sexual development genes.

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