scholarly journals Heterochromatic Stellate Gene Cluster in Drosophila melanogaster: Structure and Molecular Evolution

Genetics ◽  
1997 ◽  
Vol 146 (1) ◽  
pp. 253-262 ◽  
Author(s):  
Alexei V Tulin ◽  
Galina L Kogan ◽  
Dominik Filipp ◽  
Maria D Balakireva ◽  
Vladimir A Gvozdev
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Evolution ◽  
Protein Kinase Ck2 ◽  
Unknown Origin ◽  
Open Reading Frames ◽  
Β Subunit ◽  
Retrotransposon Insertion ◽  
High Level ◽  
Kinase Ck2 ◽  
Reading Frames

The 30-kb cluster comprising close to 20 copies of tandemly repeated Stellate genes was localized in the distal heterochromatin of the X chromosome. Of 10 sequenced genes, nine contain undamaged open reading frames with extensive similarity to protein kinase CK2 β-subunit; one gene is interrupted by an insertion. The heterochromatic array of Stellate repeats is divided into three regions by a 4.5-kb DNA segment of unknown origin and a retrotransposon insertion: the A region (∼14 Stellate genes), the adjacent B region (approximately three Stellate genes), and the C region (about four Stellate genes). The sequencing of Stellate copies located along the discontinuous cluster revealed a complex pattern of diversification. The lowest level of divergence was detected in nearby Stellate repeats. The marginal copies of the A region, truncated or interrupted by an insertion, escaped homogenization and demonstrated high levels of divergence. Comparison of copies in the B and C regions, which are separated by a retrotransposon insertion, revealed a high level of diversification. These observations suggest that homogenization takes place in the Stellate cluster, but that inserted sequences may impede this process.

A repetitive DNA element, associated with telomeric sequences in Drosophila melanogaster, contains open reading frames

Chromosoma ◽  
1992 ◽  
Vol 102 (1) ◽  
pp. 32-40 ◽  
Author(s):  
Olga N. Danilevskaya ◽  
Dmitri A. Petrov ◽  
Maria N. Pavlova ◽  
Akihiko Koga ◽  
Elena V. Kurenova ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Repetitive Dna ◽  
Open Reading Frames ◽  
Telomeric Sequences ◽  
Reading Frames

The crystal structure of the complex of Zea maysα subunit with a fragment of human β subunit provides the clue to the architecture of protein kinase CK2 holoenzyme

2000 ◽  
Vol 267 (16) ◽  
pp. 5184-5190 ◽  
Author(s):  
Roberto Battistutta ◽  
Stefania Sarno ◽  
Erika De Moliner ◽  
Oriano Marin ◽  
Olaf-G. Issinger ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Β Subunit ◽  
Kinase Ck2

scholarly journals An in vivo assay for the reverse transcriptase of human retrotransposon L1 in Saccharomyces cerevisiae.

1994 ◽  
Vol 14 (7) ◽  
pp. 4485-4492 ◽  
Author(s):  
B A Dombroski ◽  
Q Feng ◽  
S L Mathias ◽  
D M Sassaman ◽  
A F Scott ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Consensus Sequence ◽  
Unknown Origin ◽  
Open Reading Frames ◽  
Long Terminal Repeats ◽  
Base Pairs ◽  
In Vivo Assay ◽  
Reading Frames

L1 elements constitute a highly repetitive human DNA family (50,000 to 100,000 copies) lacking long terminal repeats and ending in a poly(A) tail. Some L1 elements are capable of retrotransposition in the human genome (Kazazian, H. H., Jr., C. Wong, H. Youssoufian, A. F. Scott, D. G. Phillips, and S.E. Antonarakis, Nature (London) 332:164-166, 1988). Although most are 5' truncated, a consensus sequence of complete L1 elements is 6 kb long and contains two open reading frames (ORFs) (Scott, A. F., B. J. Schmeckpeper, M. Abdelrazik, C. T. Comey, B. O'Hara, J. P. Rossiter, T. Cooley, P. Health, K. D. Smith, and L. Margolet, Genomics 1:113-125, 1987). The protein encoded by ORF2 has reverse transcriptase (RT) activity in vitro (Mathias, S. L., A. F. Scott, H. H. Kazazian, Jr., J. D. Boeke, and A. Gabriel, Science 254:1808-1810, 1991). Because L1 elements are so numerous, efficient methods for identifying active copies are required. We have developed a simple in vivo assay for the activity of L1 RT based on the system developed by Derr et al. (Derr, L. K., J. N. Strathern, and D. J. Garfinkel, Cell 67:355-364, 1991) for yeast HIS3 pseudogene formation. L1 ORF2 displays an in vivo RT activity similar to that of yeast Ty1 RT in this system and generates pseudogenes with unusual structures. Like the HIS3 pseudogenes whose formation depends on Ty1 RT, the HIS3 pseudogenes generated by L1 RT are joined to Ty1 sequences and often are part of complex arrays of Ty1 elements, multiple HIS3 pseudogenes, and hybrid Ty1/L1 elements. These pseudogenes differ from those previously described in that there are base pairs of unknown origin inserted at several of the junctions. In two of three HIS3 pseudogenes studied, the L1 RT appears to have jumped from the 5' end of a Ty1/L1 transcript to the poly(A) tract of the HIS3 RNA.

scholarly journals The regulatory β-subunit of protein kinase CK2 regulates cell-cycle progression at the onset of mitosis

Oncogene ◽  
2008 ◽  
Vol 27 (37) ◽  
pp. 4986-4997 ◽  
Author(s):  
C W Yde ◽  
B B Olsen ◽  
D Meek ◽  
N Watanabe ◽  
B Guerra
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Cell Cycle Progression ◽  
Β Subunit ◽  
Cycle Progression ◽  
Kinase Ck2

Overexpression of Nuclear Protein Kinase CK2 β Subunit and Prognosis in Human Gastric Carcinoma

2010 ◽  
Vol 17 (6) ◽  
pp. 1695-1702 ◽  
Author(s):  
Kai-Yuan Lin ◽  
Chia-Lang Fang ◽  
Yi Chen ◽  
Chien-Feng Li ◽  
Sheng-Hsuan Chen ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Gastric Carcinoma ◽  
Protein Kinase Ck2 ◽  
Nuclear Protein ◽  
Β Subunit ◽  
Human Gastric Carcinoma ◽  
Kinase Ck2

scholarly journals Differences in editing of mitochondrial nad3 transcripts from CMS and fertile carrots.

2001 ◽  
Vol 48 (3) ◽  
pp. 711-717 ◽  
Author(s):  
M Rurek ◽  
M Szklarczyk ◽  
N Adamczyk ◽  
B Michalik ◽  
H Augustyniak
Keyword(s):  
Amino Acids ◽  
Nucleotide Sequence ◽  
Sequence Conservation ◽  
Open Reading Frames ◽  
Male Sterile ◽  
Nucleotide Substitutions ◽  
Cytoplasmic Male Sterile ◽  
Fertile Line ◽  
High Level ◽  
Reading Frames

A high level of the nucleotide sequence conservation was found for mitochondrial nad3 gene of carrot. Three silent nucleotide substitutions differentiate nad3 open reading frames from cytoplasmic male sterile and male fertile carrots. All these differences are preserved on the RNA level. Partial and silent editing also distinguished both carrots. Three of the C to U conversions were specific to the fertile line. In the two examined carrot lines editing did not affect the mode of alteration of encoded amino acids.

scholarly journals Site-directed mutants of the β subunit of protein kinase CK2 demonstrate the important role of Pro-58

FEBS Letters ◽  
1995 ◽  
Vol 368 (2) ◽  
pp. 211-214 ◽  
Author(s):  
María Victoria Hinrichs ◽  
Marta Gatica ◽  
Catherine C. Allende ◽  
Jorge E. Allende
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Β Subunit ◽  
Kinase Ck2
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