scholarly journals Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian beta-1,4-galactosyltransferase and beta-1,2-N-acetylglucosaminyltransferase II

Glycobiology ◽  
2002 ◽  
Vol 13 (1) ◽  
pp. 23-34 ◽  
Author(s):  
N. Tomiya
Keyword(s):  
Trichoplusia Ni ◽  
Insect Cells ◽  
Complex Type ◽  
Human Transferrin

scholarly journals N-glycan patterns of human transferrin produced in Trichoplusia ni insect cells: effects of mammalian galactosyltransferase

Glycobiology ◽  
2000 ◽  
Vol 10 (8) ◽  
pp. 837-847 ◽  
Author(s):  
E. Ailor ◽  
N. Takahashi ◽  
Y. Tsukamoto ◽  
K. Masuda ◽  
B. A. Rahman ◽  
...  
Keyword(s):  
Trichoplusia Ni ◽  
Insect Cells ◽  
Human Transferrin

scholarly journals Bacmid Expression of Granulovirus Enhancin En3 Accumulates in Cell Soluble Fraction to Potentiate Nucleopolyhedrovirus Infection

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1233
Author(s):  
Adriana Ricarte-Bermejo ◽  
Oihane Simón ◽  
Ana Beatriz Fernández ◽  
Trevor Williams ◽  
Primitivo Caballero
Keyword(s):  
Trichoplusia Ni ◽  
Recombinant Virus ◽  
Spodoptera Exigua ◽  
Insect Cells ◽  
Soluble Fraction ◽  
Autographa Californica ◽  
Insect Midgut ◽  
Baculovirus Infection ◽  
Occlusion Bodies ◽  
Infected Cells

Enhancins are metalloproteinases that facilitate baculovirus infection in the insect midgut. They are more prevalent in granuloviruses (GVs), constituting up to 5% of the proteins of viral occlusion bodies (OBs). In nucleopolyhedroviruses (NPVs), in contrast, they are present in the envelope of the occlusion-derived virions (ODV). In the present study, we constructed a recombinant Autographa californica NPV (AcMNPV) that expressed the Trichoplusia ni GV (TnGV) enhancin 3 (En3), with the aim of increasing the presence of enhancin in the OBs or ODVs. En3 was successfully produced but did not localize to the OBs or the ODVs and accumulated in the soluble fraction of infected cells. As a result, increased OB pathogenicity was observed when OBs were administered in mixtures with the soluble fraction of infected cells. The mixture of OBs and the soluble fraction of Sf9 cells infected with BacPhEn3 recombinant virus was ~3- and ~4.7-fold more pathogenic than BacPh control OBs in the second and fourth instars of Spodoptera exigua, respectively. In contrast, when purified, recombinant BacPhEn3 OBs were as pathogenic as control BacPh OBs. The expression of En3 in the soluble fraction of insect cells may find applications in the development of virus-based insecticides with increased efficacy.

scholarly journals Lipid composition of Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) insect cells used for baculovirus infection

FEBS Letters ◽  
1998 ◽  
Vol 441 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Kathrin Marheineke ◽  
Sylvia Grünewald ◽  
William Christie ◽  
Helmut Reiländer
Keyword(s):  
Spodoptera Frugiperda ◽  
Lipid Composition ◽  
Trichoplusia Ni ◽  
Insect Cells ◽  
Baculovirus Infection

scholarly journals Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins

2020 ◽  
pp. mcp.RA120.002295 ◽  
Author(s):  
Yong Zhang ◽  
Wanjun Zhao ◽  
Yonghong Mao ◽  
Yaohui Chen ◽  
Shisheng Wang ◽  
...  
Keyword(s):  
Insect Cells ◽  
Host Cells ◽  
Protein Subunit ◽  
Genome Sequences ◽  
Complex Type ◽  
Site Specific ◽  
Differential Processing ◽  
S Protein ◽  
Shed Light

The glycoprotein spike (S) on the surface of SARS-CoV-2 is a determinant for viral invasion and host immune response. Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides. All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences. The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunit. We identified masses that correspond to 157 N-glycans, primarily of the complex type. In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type. Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites’ location. Moreover, the N-glycan compositions were conserved among different sizes of subunits. Our study indicate that the S protein N-glycosylation occurs regularly at each site, albeit the occupied N-glycans were diverse and heterogenous. This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.

Insights into the Central Metabolism of Spodoptera frugiperda (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4(Tn-5) Insect Cells by Radiolabeling Studies

2008 ◽  
Vol 21 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Chouki Benslimane ◽  
Cynthia B. Elias ◽  
Jalal Hawari ◽  
Amine Kamen
Keyword(s):  
Spodoptera Frugiperda ◽  
Trichoplusia Ni ◽  
Insect Cells ◽  
Central Metabolism

Optimization of Critical Medium Components for the Expression of Recombinant Human Transferrin in Insect Cells Baculovirus System

2012 ◽  
Author(s):  
Clarence M. Ongkudon ◽  
Badarulhisam Abdul Rahman ◽  
Azila Abd. Aziz
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Central Composite Design ◽  
Response Surface ◽  
Insect Cells ◽  
Expression System ◽  
Proliferating Cells ◽  
Human Transferrin ◽  
Lipid Mixtures ◽  
Central Composite

Transferin manusia (hTf) memainkan peranan yang penting dalam fungsi bakteriostatik dan pengangkutan ferum dari bahagian penyimpanan ke sel–sel yang membiak melalui proses endositosis janaan reseptor. Sistem ekspresi bakulovirus sel serangga telah dipakai secara meluas sebagai sistem alternatif dalam penghasilan Transferin manusia rekombinan (rhTf). Kajian ini ditumpukan ke atas pengoptimuman glutamina, glukosa dan campuran lipid 1000x yang dapat meningkatkan penghasilan rhTf. Reka bentuk eksperimen yang melibatkan 17 eksperimen reka bentuk komposit berpusat (CCD) telah digunakan dan hasil kajian dianalisis oleh Statistika (Statsoft v. 5.0). Metodologi permukaan tindak balas (RSM) telah mengenalpasti nilai optimum parameterparameter yang dikaji iaitu glutamina=2211.20 mg/L, glukosa=1291.95 mg/L, dan campuran lipid 1000x=0.64 %v/v. Hasil optimasi menunjukkan peningkatan hasil rhTf sebanyak tiga kali ganda, iaitu daripada 19.89 μg/ml kepada 65.12 μg/ml. Kata kunci: Transferin manusia; bakulovirus sel serangga; reka bentuk eksperimen; reka bentuk komposit berpusat; metodologi permukaan tindak balas Human Transferrin (hTf) plays a big role in providing bacteriostatic functions as well as to transport iron from the storage part to all proliferating cells by receptor mediated endocytosis. Insect cells baculovirus expression system has been widely used as an alternative expression system for the production of recombinant human Transferrin (rhTf). This work focused mainly on the optimization of glutamine, glucose and lipid mixtures 1000x to increase rhTf yield. An experimental design involving 17 central composite design (CCD) experiments was employed and results were analyzed by Statistica (Statsoft v. 5.0). The response surface methodology (RSM) had identified the optimum values where glutamine=2211.20 mg/L, glucose=1291.95 mg/L, and lipid mixtures 1000x=0.64 %v/v. Using the optimized parameters, the studies demonstrated an increase in the rhTf yield by three–fold from 19.89 μg/ml to 65.12 μg/ml. Key words: Human transferrin; insect cells baculovirus; experimental design; central composite design; response surface methodology

Improving Glucose and Glutamine Metabolism of Human HEK 293 and Trichoplusia ni Insect Cells Engineered To Express a Cytosolic Pyruvate Carboxylase Enzyme

2003 ◽  
Vol 19 (1) ◽  
pp. 90-97 ◽  
Author(s):  
C.B. Elias ◽  
E. Carpentier ◽  
Y. Durocher ◽  
L. Bisson ◽  
R. Wagner ◽  
...  
Keyword(s):  
Trichoplusia Ni ◽  
Pyruvate Carboxylase ◽  
Insect Cells ◽  
Glutamine Metabolism ◽  
Hek 293

scholarly journals Glycosylation in Lepidopteran insect cells: identification of a β1→4-N-acetylgalactosaminyltransferase involved in the synthesis of complex-type oligosaccharide chains

Glycobiology ◽  
1996 ◽  
Vol 6 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Irma van Die ◽  
Angelique van Tetering ◽  
Hans Bakker ◽  
Dirk H. van den Eijnden ◽  
David H. Joziasse
Keyword(s):  
Insect Cells ◽  
Complex Type ◽  
Lepidopteran Insect

Overexpression of a functional NMDA receptor subunit (NMDAR1) in baculovirus-infected Trichoplusia ni insect cells

1996 ◽  
Vol 41 (1-2) ◽  
pp. 228-240 ◽  
Author(s):  
Sabine Sydow ◽  
Andreas K.E Köpke ◽  
Thomas Blank ◽  
Joachim Spiess
Keyword(s):  
Nmda Receptor ◽  
Trichoplusia Ni ◽  
Insect Cells ◽  
Receptor Subunit ◽  
Nmda Receptor Subunit
Sign in / Sign up

Export Citation Format

Share Document