Glycine transport by single human and mouse embryos

Key Points Expression of caudal-related homeobox gene 2 (CDX2) and angiotensin-converting enzyme (ACE) correlates during hematopoietic emergence. This emergence occurs in human and mouse embryos and in human acute myeloid leukemia; CDX2 homeoprotein also binds to the ACE promoter.

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De novo deletions of SNRPN exon 1 in early human and mouse embryos result in a paternal to maternal imprint switch

Nature Genetics ◽

10.1038/75629 ◽

2000 ◽

Vol 25 (1) ◽

pp. 74-78 ◽

Cited By ~ 95

Author(s):

Beata Bielinska ◽

Susan M. Blaydes ◽

Karin Buiting ◽

Tao Yang ◽

Malgorzata Krajewska-Walasek ◽

...

Keyword(s):

De Novo ◽

Mouse Embryos ◽

Exon 1 ◽

Human And Mouse ◽

Early Human

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Apoptosis during inner ear development in human and mouse embryos: an analysis by computer-assisted three-dimensional reconstruction

Anatomy and Embryology ◽

10.1007/s004290050255 ◽

1999 ◽

Vol 200 (1) ◽

pp. 19-26 ◽

Cited By ~ 35

Author(s):

T. Nishikori ◽

Toshihisa Hatta ◽

Hideyuki Kawauchi ◽

Hiroki Otani

Keyword(s):

Inner Ear ◽

Three Dimensional ◽

Mouse Embryos ◽

Computer Assisted ◽

Three Dimensional Reconstruction ◽

Ear Development ◽

Inner Ear Development ◽

Dimensional Reconstruction ◽

Human And Mouse

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RERE deficiency contributes to the development of orofacial clefts in humans and mice

Human Molecular Genetics ◽

10.1093/hmg/ddab084 ◽

2021 ◽

Author(s):

Bum Jun Kim ◽

Hitisha P Zaveri ◽

Peter N Kundert ◽

Valerie K Jordan ◽

Tiana M Scott ◽

...

Keyword(s):

Cleft Palate ◽

Cleft Lip ◽

Neurodevelopmental Disorder ◽

Mouse Embryos ◽

Deletion Syndrome ◽

Orofacial Clefts ◽

Orofacial Clefting ◽

Increased Risk ◽

The Brain ◽

Human And Mouse

AbstractDeletions of chromosome 1p36 are the most common telomeric deletions in humans and are associated with an increased risk of orofacial clefting. Deletion/phenotype mapping, combined with data from human and mouse studies, suggests the existence of multiple 1p36 genes associated with orofacial clefting including SKI, PRDM16, PAX7 and GRHL3. The arginine–glutamic acid dipeptide (RE) repeats gene (RERE) is located in the proximal critical region for 1p36 deletion syndrome and encodes a nuclear receptor co-regulator. Pathogenic RERE variants have been shown to cause neurodevelopmental disorder with or without anomalies of the brain, eye or heart (NEDBEH). Cleft lip has previously been described in one individual with NEDBEH. Here we report the first individual with NEDBEH to have a cleft palate. We confirm that RERE is broadly expressed in the palate during mouse embryonic development, and we demonstrate that the majority of RERE-deficient mouse embryos on C57BL/6 background have cleft palate. We go on to show that ablation of Rere in cranial neural crest (CNC) cells, mediated by a Wnt1-Cre, leads to delayed elevation of the palatal shelves and cleft palate and that proliferation of mesenchymal cells in the palatal shelves is significantly reduced in Rereflox/flox; Wnt1-Cre embryos. We conclude that loss of RERE function contributes to the development of orofacial clefts in individuals with proximal 1p36 deletions and NEDBEH and that RERE expression in CNC cells and their derivatives is required for normal palatal development.

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Vitrification of human and mouse embryos using the Rapid-i™

Fertility and Sterility ◽

10.1016/j.fertnstert.2010.07.471 ◽

2010 ◽

Vol 94 (4) ◽

pp. S115

Author(s):

B. Balaban ◽

A. Isiklar ◽

B. Urman ◽

D.K. Gardner ◽

M.G. Larman

Keyword(s):

Mouse Embryos ◽

Human And Mouse

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Selection of reference genes in mouse embryos and in differentiating human and mouse ES cells

The International Journal of Developmental Biology ◽

10.1387/ijdb.052130ew ◽

2006 ◽

Vol 50 (7) ◽

pp. 627-635 ◽

Cited By ~ 74

Author(s):

Erik Willems ◽

Ileana Mateizel ◽

Caroline Kemp ◽

Greet Cauffman ◽

Karen Sermon ◽

...

Keyword(s):

Reference Genes ◽

Es Cells ◽

Mouse Embryos ◽

Mouse Es Cells ◽

Human And Mouse ◽

Selection Of

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Global, Survival, and Apoptotic Transcriptome during Mouse and Human Early Embryonic Development

BioMed Research International ◽

10.1155/2018/5895628 ◽

2018 ◽

Vol 2018 ◽

pp. 1-16 ◽

Cited By ~ 3

Author(s):

D. Haouzi ◽

I. Boumela ◽

K. Chebli ◽

S. Hamamah

Keyword(s):

Embryonic Development ◽

Molecular Mechanisms ◽

Expression Profiles ◽

Mouse Embryos ◽

Signalling Pathways ◽

Human Embryos ◽

Special Focus ◽

Mammalian Embryo ◽

Human And Mouse ◽

Early Human

Survival and cell death signals are crucial for mammalian embryo preimplantation development. However, the knowledge on the molecular mechanisms underlying their regulation is still limited. Mouse studies are widely used to understand preimplantation embryo development, but extrapolation of these results to humans is questionable. Therefore, we wanted to analyse the global expression profiles during early mouse and human development with a special focus on genes involved in the regulation of the apoptotic and survival pathways. We used DNA microarray technology to analyse the global gene expression profiles of preimplantation human and mouse embryos (metaphase II oocytes, embryos at the embryonic genome activation stage, and blastocysts). Components of the major apoptotic and survival signalling pathways were expressed during early human and mouse embryonic development; however, most expression profiles were species-specific. Particularly, the expression of genes encoding components and regulators of the apoptotic machinery were extremely stable in mouse embryos at all analysed stages, while it was more stage-specific in human embryos.CASP3,CASP9, andAIFwere the only apoptosis-related genes expressed in both species and at all studied stages. Moreover, numerous transcripts related to the apoptotic and survival pathway were reported for the first time such asCASP6andIL1RAPL1that were specific to MII oocytes;CASP2,ENDOG, andGFERto blastocysts in human. These findings open new perspectives for the characterization and understanding of the survival and apoptotic signalling pathways that control early human and mouse embryonic development.

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Identification of the Lineage Markers and Inhibition of DAB2 in In Vitro Fertilized Porcine Embryos

International Journal of Molecular Sciences ◽

10.3390/ijms21197275 ◽

2020 ◽

Vol 21 (19) ◽

pp. 7275

Author(s):

Jong-Nam Oh ◽

Mingyun Lee ◽

Gyung Cheol Choe ◽

Dong-Kyung Lee ◽

Kwang-Hwan Choi ◽

...

Keyword(s):

Low Cost ◽

Expression Patterns ◽

Mouse Embryos ◽

Marker Genes ◽

Lineage Specification ◽

Marker Proteins ◽

Lineage Markers ◽

Human And Mouse

Specification of embryonic lineages is an important question in the field of early development. Numerous studies analyzed the expression patterns of the candidate transcripts and proteins in humans and mice and clearly determined the markers of each lineage. To overcome the limitations of human and mouse embryos, the expression of the marker transcripts in each cell has been investigated using in vivo embryos in pigs. In vitro produced embryos are more accessible, can be rapidly processed with low cost. Therefore, we analyzed the characteristics of lineage markers and the effects of the DAB2 gene (trophectoderm marker) in in vitro fertilized porcine embryos. We investigated the expression levels of the marker genes during embryonic stages and distribution of the marker proteins was assayed in day 7 blastocysts. Then, the shRNA vectors were injected into the fertilized embryos and the differences in the marker transcripts were analyzed. Marker transcripts showed diverse patterns of expression, and each embryonic lineage could be identified with localization of marker proteins. In DAB2-shRNA vectors injected embryos, HNF4A and PDGFRA were upregulated. DAB2 protein level was lower in shRNA-injected embryos without significant differences. Our results will contribute to understanding of the mechanisms of embryonic lineage specification in pigs.

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