Towards non-surgical therapy for uterine fibroids: catechol-O-methyl transferase inhibitor shrinks uterine fibroid lesions in the Eker rat model

Uterine fibroid tissues are often compared to their matched myometrium in an effort to understand their pathophysiology, but it is not clear whether the myometria of uterine fibroid patients represent truly non-disease control tissues. We analyzed the transcriptomes of myometrial samples from non-fibroid patients (M) and compared them with fibroid (F) and matched myometrial (MF) samples to determine whether there is a phenotypic difference between fibroid and non-fibroid myometria. Multidimensional scaling plots revealed that M samples clustered separately from both MF and F samples. A total of 1169 differentially expressed genes (DEGs) (false discovery rate < 0.05) were observed in the MF comparison with M. Overrepresented Gene Ontology terms showed a high concordance of upregulated gene sets in MF compared to M, particularly extracellular matrix and structure organization. Gene set enrichment analyses showed that the leading-edge genes from the TGFβ signaling and inflammatory response gene sets were significantly enriched in MF. Overall comparison of the three tissues by three-dimensional principal component analyses showed that M, MF, and F samples clustered separately from each other and that a total of 732 DEGs from F vs. M were not found in the F vs. MF, which are likely understudied in the pathogenesis of uterine fibroids and could be key genes for future investigation. These results suggest that the transcriptome of fibroid-associated myometrium is different from that of non-diseased myometrium and that fibroid studies should consider using both matched myometrium and non-diseased myometrium as controls.

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“Second hit” of Tsc2 gene in radiation induced renal tumors of Eker rat model

International Congress Series ◽

10.1016/s0531-5131(02)00155-3 ◽

2002 ◽

Vol 1236 ◽

pp. 163-174 ◽

Cited By ~ 7

Author(s):

Okio Hino ◽

Hiroaki Mitani ◽

Junko Sakaurai

Keyword(s):

Rat Model ◽

Renal Tumors ◽

Tsc2 Gene ◽

Second Hit ◽

Eker Rat ◽

Radiation Induced

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TO THE QUESTION ON UTERINE FIBROIDS DURING PREGNANCY

Scientific digest of association of obstetricians and gynecologists of Ukraine ◽

10.35278/2664-0767.1(47).2021.242698 ◽

2021 ◽

pp. 54-59

Author(s):

T. F. TATARCHUK ◽

N. V. KOSEY ◽

S. I. REGEDA ◽

O. V. ZANKO ◽

K. D. PLAKSIIEVA

Keyword(s):

Statistical Analysis ◽

Cesarean Section ◽

Live Birth ◽

Uterine Fibroid ◽

Postpartum Period ◽

Uterine Fibroids ◽

Premature Delivery ◽

Pelvic Ultrasound ◽

Natural Childbirth ◽

Increased Risk

Uterine fibroids is an extremely common tumor of the female reproductive system, among whose manifestations are infertility, spontaneous abortions, incorrect fetal position, placenta previa, premature delivery, bleeding during and after delivery, and an increased risk of cesarean section. According to the literature, myomas are changing in size during pregnancy and in the postpartum period. Aim of the study. To assess the dynamics of uterine fibroid size change during pregnancy and the effect of an existing uterine fibroid on the course of pregnancy and labor. Materials and methods. Outpatient records of patients aged 24 to 45 years (mean age 33.36 ± 4.63 years) who were diagnosed with Pregnancy and uterine fibroids from 2016 to 2021 at Verum Medical Center were evaluated (n = 57). The size of the fibroids (volume and diameter) before, during (I or II prenatal screening) and after pregnancy (first pelvic ultrasound after delivery) was used for statistical analysis. Forty-one of the 57 patients had pelvic ultrasound before, during, and after pregnancy and were included in the statistical analysis of changes in uterine myoma size. Results. Among the patients included in the statistical analysis, uterine fibroids increased in volume by 194.38% ± 86.9 (40.98% ± 18.4 in diameter) during pregnancy, and decreased by 53.98% ± 14.93 in diameter and by 54.28% ± 29.62 of baseline in the postpartum period. A significant number of fibroids (39.47%) did undergo involutionary changes and were not visualized in the first postpartum ultrasound. The live birth rate was high at 90% (64% of deliveries were through natural childbirth and 26% through cesarean section). Conclusions. There was no effect of intramural, intramural-subserosal, and subserosal uterine fibroids with an average diameter of £20 mm on pregnancy and live birth in women. A great amount of uterine fibroids nearly triple in size during pregnancy, but after delivery they return to their original size and even halve in size. This can be regarded as a confirmation of the absence of a negative effect of pregnancy, or, possibly, a positive effect on uterine fibroid size, which requires further investigation.

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Concept of Granthi with reference to uterine fibroid: An Ayurveda review

National Journal of Research in Ayurved Science ◽

10.52482/ayurlog.v9i02.840 ◽

2021 ◽

Vol 9 (02) ◽

Author(s):

Jaya Umate ◽

Soudamini Chaudhari

Keyword(s):

Life Span ◽

Uterine Fibroid ◽

Uterine Fibroids ◽

Modern Science ◽

Reproductive Age ◽

Significant Morbidity ◽

Reproductive Life ◽

Reproductive Life Span ◽

Gynecological Disease ◽

Reproductive Age Group

Uterine fibroids are the commonest benign tumor of the uterus and also the commonest benign solid tumor in the female. It can cause significant morbidity in women of a reproductive life span. The exact cause of uterine fibroid is unknown. Prevalence of uterine fibroid 5 -20 % of women in the reproductive age group. It can cause significant morbidity in women of a reproductive life span. Not all fibroids cause symptoms. 50% of women are asymptomatic. Fibroids mainly cause symptoms like menorrhagia, dysmenorrhea and cause pressure symptoms on adjacent viscera i. e. bladder, uterus, rectum which affects the everyday activities of women. In Ayurveda all gynecological disease explained under the term yonivyapad. It can be explained as the anatomical and functional abnormalities of the female reproductive system. In Ayurveda granthi can be correlated with the uterine fibroid. In modern science uterine fibroid treats medically, surgically. But it is challenging to establish a satisfactory conservatory medical treatment to date. so in Ayurveda the main purpose is the management of granthi by samprapti vighatan by use of medicine.

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