Introduction- HPTLC uses include phytochemical and biochemical analyzes, ayurvedic medicine quantification and quantification of active ingredients, formational fingerprinting and adulterant testing of formulations. HPTLC can be used as a simple tool for tracking the consistency of plant-based raw plant materials and formulations. The source of many biomarkers is Sammoha Loha Vati. The HPTLC method has been used to classify and measure the bark of leaves and branches. Aim & Objective- Identification and authentication of raw drugs used for Sammoha Loha Vati through HPTLC. Observations- 1. Stationary phase, 2. Mobile phase, 3. Optimization, 4. Preparation of Sample and its use, 5. Separation, 6. Detection. Conclusion and Finding- The use of HPTLC for the screening of pharmaceutical compounds for antimicrobial activities is emerging. Requirements for the testing of new incoming products and their integration into regulatory frameworks are of great significance for the future of HPTLC. Rf Value having at 254 nm Chromatogram, Spot No. [1] Track T1 [0.22], Track T2 [0.22], Rf Value having at 366 nm Chromatogram, Spot No. [1] Track T1 [0.10], Track T2 [0.10], Rf Value having at 540 nm Chromatogram, Spot No.[1] Track T1 [0.10], Track T2 [0.10].