scholarly journals Engineering the ovarian cycle using in vitro follicle culture

2015 ◽  
Vol 30 (6) ◽  
pp. 1386-1395 ◽  
Author(s):  
Robin M. Skory ◽  
Yuanming Xu ◽  
Lonnie D. Shea ◽  
Teresa K. Woodruff
Keyword(s):  
Ovarian Cycle ◽  
Follicle Culture ◽  
In Vitro Follicle Culture

Proliferation and Differentiation of Theca-Like Cells from Granulosa Cells During In Vitro Follicle Culture.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 706-706
Author(s):  
Min Xu ◽  
Erin Jackson ◽  
Lonnie D. Shea ◽  
Teresa K Woodruff
Keyword(s):  
Granulosa Cells ◽  
Follicle Culture ◽  
Proliferation And Differentiation ◽  
In Vitro Follicle Culture

scholarly journals Ovarian Follicles Rescued 3 Days after Cyclophosphamide Treatment in Adolescent Mice: An Experimental Study Aiming at Maximizing Methods for Fertility Preservation through In Vitro Follicle Culture

2019 ◽  
Vol 20 (24) ◽  
pp. 6190
Author(s):  
Amandine Anastácio ◽  
Max Waterstone ◽  
Xia Hao ◽  
Catherine Poirot ◽  
Kenny A. Rodriguez-Wallberg
Keyword(s):  
Fertility Preservation ◽  
Survival Rates ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Controlled Study ◽  
Phosphatase And Tensin Homolog ◽  
Follicle Culture ◽  
Tensin Homolog ◽  
Cyclophosphamide Treatment ◽  
In Vitro Follicle Culture

There is currently a lack of knowledge about the feasibility of performing procedures for fertility preservation after chemotherapy treatment has been initiated. In this experimental controlled study using adolescent mice, we aimed to investigate if the chance of rescuing and growing in vitro secondary follicles (SeF) would be affected three days after a single injection of cyclophosphamide (CPA). The main outcomes included were: (1) The number of SeF with good morphologic quality obtained per ovary 3 days after CPA injection, (2) SeF development in culture, (3) small follicle density (SFD) on histology, and (4) apoptosis markers, including terminal deoxynucleotidyl transferase dUTP nick end-labelling (TUNEL), mRNA expression, and distribution of p 53 upregulated modulator of apoptosis (Puma) and phosphatase and tensin homolog (Pten). We found a 60% reduction of SeF obtained per ovary in all CPA-treated groups vs. controls. However, in vitro survival rates at culture day 12 and antrum formation were similar among all groups. On histology, SFD was only significantly reduced in the high CPA dose group. Apoptotic cells were mainly found in large growing follicles of CPA groups. Our study indicates the feasibility of SeF isolation and in vitro follicle culture 3 days following CPA treatment and a still preserved SFD, particularly following a low-dose CPA treatment.

scholarly journals Advances in the applications of polymer biomaterials for in vitro follicle culture

2021 ◽  
Vol 140 ◽  
pp. 111422
Author(s):  
Du Xiang ◽  
Yang Liu ◽  
Encheng Zhou ◽  
Yanfeng Wang
Keyword(s):  
Follicle Culture ◽  
In Vitro Follicle Culture

Three dimensional (3-D) in vitro follicle culture system of secondary follicles in cycling mice: obesity and cycle stage may impact oocyte quality

2009 ◽  
Vol 92 (3) ◽  
pp. S8
Author(s):  
J.E. Hirshfeld-Cytron ◽  
J.K. Jozefik ◽  
M. Xu ◽  
A. Shikanov ◽  
L.D. Shea ◽  
...  
Keyword(s):  
Culture System ◽  
Three Dimensional ◽  
Oocyte Quality ◽  
Follicle Culture ◽  
In Vitro Follicle Culture

scholarly journals Co-Culturing Primary Follicles Promotes Survival and Growth During Three-dimensional In Vitro Follicle Culture.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 663-663
Author(s):  
Francesca E. Duncan ◽  
Jessica E. Hornick ◽  
Erin Jackson ◽  
Teresa Woodruff
Keyword(s):  
Three Dimensional ◽  
Follicle Culture ◽  
Survival And Growth ◽  
In Vitro Follicle Culture

The potentiality of two-dimensional preantral follicle culture as an in vitro model in predicting premature ovarian failure

2017 ◽  
Vol 69 (7) ◽  
pp. 477-484 ◽  
Author(s):  
Ting Zhang ◽  
Ying Chen ◽  
Yang Yang ◽  
Zhonghui Wang ◽  
Qi Pan ◽  
...  
Keyword(s):  
Premature Ovarian Failure ◽  
In Vitro Model ◽  
Ovarian Failure ◽  
Two Dimensional ◽  
Preantral Follicle ◽  
Follicle Culture ◽  
Vitro Model

scholarly journals The production of mature oocytes from adult ovaries following primary follicle culture in a marsupial

Reproduction ◽  
2009 ◽  
Vol 138 (2) ◽  
pp. 247-255 ◽  
Author(s):  
A Nation ◽  
L Selwood
Keyword(s):  
Normal Growth ◽  
Primary Follicle ◽  
Follicle Culture ◽  
Nuclear Maturation ◽  
Transmission Electron ◽  
Progressive Growth ◽  
Sminthopsis Macroura ◽  
First Time

A model marsupial culture system has been developed whereby individual primary follicles, obtained from adult ovaries, can be grown in vitro to the antral stage and oocytes retrieved from these follicles can achieve nuclear maturation (metaphase II) in the presence of LH. Primary follicles isolated from adult Sminthopsis macroura ovaries were cultured individually in one of four systems: microdrops under oil, upright, inverted, or roller culture. After 6 days of culture, cumulus–oocyte complexes (COCs) were excised from early antral follicles and incubated for an additional 24 h to assess meiotic competence and the effects of LH and lithium on oocyte maturation. Histology and transmission electron microscopy established normal in vivo standards and verified oocyte and follicular integrity following culture. On day 6 of culture, follicle viability was significantly greater in the inverted system (73%) than in the other three systems (10–46%). The inverted system was the most effective in supporting development with follicles demonstrating progressive growth during culture and showing antral signs by day 4. Meiotic resumption during COC culture was facilitated by LH, but hindered by lithium. The ability to resume meiosis and progress to metaphase II was equivalent in oocytes retrieved following follicle culture and those matured in vivo. This study highlights the importance of oxygen and nutrient availability during marsupial follicle culture, and demonstrates for the first time that primary follicles isolated from adult mammalian ovaries can undergo normal growth and development in vitro, to produce mature, meiotically competent oocytes.

Three-dimensional in vitro ovarian follicle culture

2011 ◽  
pp. 167-176
Author(s):  
Eugene Galdones ◽  
Lonnie D. Shea ◽  
Teresa K. Woodruff
Keyword(s):  
Ovarian Follicle ◽  
Three Dimensional ◽  
Follicle Culture
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