Collaborative Study of a Spiral Vessel Count Method for Estimating Shell in the Chocolate Component of Cocoa and Related Products
Abstract Part-I: The spiral vessel count method for estimating pectic acid in cocoa has been modified to replace the saturated borax solution by 4% NaOH, which gives a clearer product to count, and to include a method for preparing the sample dry fat-free and 250 mesh before analyzing, by hand grinding or grinding by two different grinders. Counts have been made on known shell in the chocolate component mixtures. A formula usable in a 1—15% range and standard curves have been prepared for the estimation of shell in the chocolate component. Counts have been taken at 100X and 200x on a 0.2 g/100 ml concentration, and at 200X on a 0.35 g/50 ml concentration; 200X counting of the higher concentration is preferred for 1—15% shell. Cocoas of known pectic acid content were analyzed by the method and per cent shell found by spiral vessel counts compared with per cent pectic acid. These cocoas were also analyzed before being made 250 mesh for comparative purposes. An intralaboratory study was made on two samples. Part-II: A collaborative study for determining per cent shell by the spiral vessel count method was made on four samples of cocoa products by six collaborators; two samples were defatted and ground to 250 mesh, the other two samples were hand-ground. Five collaborators varied less than 1 standard deviation unit from the average on the four subdivisions; one collaborator was just outside the standard deviation on one sample. The precision of the results was within a reasonable range for a microscopic counting procedure, and the method has been recommended for adoption as official, first action.