Quantitative Determination of Tremortins in Agricultural Commodities

1971 ◽  
Vol 54 (5) ◽  
pp. 1035-1036
Author(s):  
C T Hou ◽  
A Ciegler ◽  
C W Hesseltine
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Thin Layer Chromatography ◽  
Colorimetric Assay ◽  
Agricultural Commodities ◽  
Agricultural Commodity ◽  
Layer Chromatography ◽  
Chloroform Methanol

Abstract The procedure described for the quantitative determination of tremortins in agricultural commodities involves extracting the agricultural commodity with chloroform-methanol (2 + 1), followed by thin layer chromatography and colorimetric assay. This procedure was successfully applied to the analysis of tremortin A added to grains and the production of tremortins A and B on various commodities.

Simple Colorimetric Estimation of Cyclopiazonic Acid in Contaminated Food and Feeds

1984 ◽  
Vol 67 (1) ◽  
pp. 38-40
Author(s):  
A Rathinavelu ◽  
Edayathimangalam Rajabhavani B Shanmugasundaram
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Thin Layer Chromatography ◽  
Minimal Medium ◽  
Colorimetric Assay ◽  
Cyclopiazonic Acid ◽  
Assay Procedure ◽  
Contaminated Food ◽  
Layer Chromatography

Abstract A method has been developed for quantitative determination of cyclopiazonic acid, a mycotoxin produced by a common food contaminant, Penicillium cyclopium. The organism was grown successively in synthetic minimal medium, rice, corn, and wheat for 15 days. The toxin was extracted with chloroform followed by separation by thin layer chromatography. A colorimetric assay procedure has been successfully developed for the analysis of cyclopiazonic acid present in infected rice, corn, and wheat. The sensitivity of the method was tested by using recovery experiments.

Thin Layer Chromatography Reflectometric Determination of Benzoic and Sorbic Acids in Fruit Beverages

1980 ◽  
Vol 63 (3) ◽  
pp. 675-678 ◽  
Author(s):  
Bernadette Mandrou ◽  
Francoise Bressolle
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Thin Layer Chromatography ◽  
Internal Standard ◽  
Trace Level ◽  
Layer Chromatography ◽  
Chloroform Methanol ◽  
Trace Level Determination

Abstract A procedure is proposed for the quantitative determination of both benzoic and sorbic acids in fruit beverages. This method is carried out by direct ultraviolet spectrophotometric measurements in silica chromatoplates after development with chloroform-methanol-water (65+35+7) and makes use of an internal standard. Applied to samples spiked with benzoic and sorbic acids, this technique gives accurate and reliable results with good recoveries. The sensitivity (about 1 ppm) is particularly convenient for trace level determination.

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