Trace level determination of analyte is an extremely important phenomenon and highly relevant area of environmental science, biology, medicine and pharmacology. In this review we have described different type of chemosensor that have been used so far for trace level determination of different types of analyte. Applications of chemo sensor in the arsenic determination have been discussed here. Determination of amino acid by fluorescence spectroscopy has been depicted. Detection of poisonous organic analytes by chemosensor have been made. Some chemosensors have been found to be effective to the determination of selective cations. Various techniques used for determination of analyte by using chemo sensor as well as probable sensing mechanism have been explained here.