A Screening Method for Protein Characterization and Differentiation

1976 ◽  
Vol 59 (3) ◽  
pp. 584-590
Author(s):  
Gerson F Pinto ◽  
Vera L A Costa-Carvalho ◽  
Edna R Souza ◽  
Julio S Araújo Neto
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Chromatographic Method ◽  
Screening Method ◽  
Protein Characterization ◽  
Acid Water ◽  
Acetic Acid Water ◽  
Circular Paper ◽  
Excellent Reproducibility

Abstract A circular paper chromatographic method was developed for the separation of the amino acids in proteins into 7 subgroups. Butanol-acetic acid-water (4+1+1) was used as the developing solvent. Eluted ninhydrin-stained aminograms gave rise to graphic profiles or numerical indexes based on absorbance percentages. The profiles can be used to compare protein-containing samples. Twenty different samples were studied through 190 comparisons of graphic profiles and coefficients of correlation, with only 4% misleading results. The method showed excellent reproducibility for the identification or differentiation of proteins and has the advantage of being performed with low-priced apparatus and reagents.

Liquid Structure of Acetic Acid−Water and Trifluoroacetic Acid−Water Mixtures Studied by Large-Angle X-ray Scattering and NMR

2007 ◽  
Vol 111 (31) ◽  
pp. 9270-9280 ◽  
Author(s):  
Toshiyuki Takamuku ◽  
Yasuhiro Kyoshoin ◽  
Hiroshi Noguchi ◽  
Shoji Kusano ◽  
Toshio Yamaguchi
Keyword(s):  
Acetic Acid ◽  
Trifluoroacetic Acid ◽  
Large Angle ◽  
Liquid Structure ◽  
Acid Water ◽  
X Ray ◽  
X Ray Scattering ◽  
Acetic Acid Water ◽  
Ray Scattering

Acetic acid/water separation by pervaporation with silica filled PDMS membrane

2011 ◽  
Vol 51 (5) ◽  
pp. 819-825 ◽  
Author(s):  
Housheng Hong ◽  
Longxiang Chen ◽  
Qingwen Zhang ◽  
Zheran Zhang
Keyword(s):  
Acetic Acid ◽  
Acid Water ◽  
Pdms Membrane ◽  
Water Separation ◽  
Acetic Acid Water

Diagonal Chromatography for the Selective Purification of Tyrosyl Peptides

1974 ◽  
Vol 52 (11) ◽  
pp. 1013-1017 ◽  
Author(s):  
William H. Cruickshank ◽  
Barry L. Malchy ◽  
Harvey Kaplan
Keyword(s):  
Acetic Acid ◽  
Stationary Phase ◽  
Enzymatic Digestion ◽  
Acid Water ◽  
Chromatographic Purification ◽  
Amino Groups ◽  
Chromatographic System ◽  
Original Direction ◽  
Acetic Acid Water ◽  
Citraconic Anhydride

Thiolysis of an O-dinitrophenyl-tyrosyl peptide results in an increased solubility in the stationary phase of a n-butanol – acetic acid – water – pyridine (15:3:12:10) (BAWP) paper chromatographic system. It is shown that this property can be used to form the basis of a diagonal paper chromatographic purification of tyrosyl peptides from enzymatic digests of proteins. The amino groups of the protein are first reacted with citraconic anhydride and then the citraconyl protein is reacted with 1-fluoro-2,4-dinitrobenzene. The dinitrophenyl-citraconyl protein is subjected to enzymatic digestion, applied to a strip of Whatman 3 MM paper, thiolyzed with 5% 2-mercaptoethanol in acetone, and subjected to chromatography using BAWP as solvent. A guide strip is removed, thiolyzed with 5% 2-mercaptoethanol in 25% pyridine, and resubjected to chromatography in BAWP at right angles to the original direction of chromatography. The tyrosyl peptides are displaced off the diagonal towards the origin. The off-diagonal peptides are isolated from the original chromatogram by thiolysis and chromatography using the diagonal chromatogram to locate the positions of the dinitrophenyl-tyrosyl peptides.

scholarly journals Study of Regularities and Optimization of the Process of Fir Wood Peroxide Delignification in the Medium of “Acetic Acid – Water” in the Presence of Sulfuric Acid Catalyst

2019 ◽  
pp. 590-603
Author(s):  
Olga V. Yatsenkova ◽  
Andrei M. Skripnikov ◽  
Boris N. Kuznetsov
Keyword(s):  
Acetic Acid ◽  
Lignin Content ◽  
Reaction Medium ◽  
Acid Catalyst ◽  
Acid Water ◽  
High Yield ◽  
Process Conditions ◽  
Residual Lignin ◽  
Acetic Acid Water ◽  
Peroxide Delignification

The work describes a one-stage method of cellulose obtaining from fir wood based on peroxide delignification of wood under mild conditions (100 °C, atmospheric pressure) in the presence of acetic acid, water and catalyst of 2% wt. H2SO4. The possibility of obtaining cellulose with a residual lignin content <1% wt. at a low concentration of hydrogen peroxide (3% wt.) in the reaction medium was established. The optimal concentrations of reagents (Н2О2 – 3% wt., CH3COOH – 38.9% wt.) and the duration of the process (4 h) were determined by experimental and calculation methods. This conditions provide a high yield of cellulose product (≥45% wt.) with a low content of residual lignin (<1% wt.). The composition and structure of fir cellulose was studied by chemical analysis and by FTIR and SEM methods. The cellulosic product which was obtained in optimal process conditions is high-quality cellulose

Ternary liquid equilibria system: Acetic acid + water + toluene

2007 ◽  
Vol 23 (5) ◽  
pp. 323-328 ◽  
Author(s):  
P. Dakshinamurty ◽  
V. Subrahmanyam ◽  
M. Narsimha Rao
Keyword(s):  
Acetic Acid ◽  
Acid Water ◽  
Acetic Acid Water
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