Separation of some amino acids using ethanol-acetic acid-water: A student experiment in paper chromatography

1956 ◽  
Vol 33 (3) ◽  
pp. 140 ◽  
Author(s):  
Lynore Slaten ◽  
Myra Willard ◽  
Sister Agnes Ann Green
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Paper Chromatography ◽  
Acid Water ◽  
Ethanol Acetic Acid ◽  
Acetic Acid Water

A Screening Method for Protein Characterization and Differentiation

1976 ◽  
Vol 59 (3) ◽  
pp. 584-590
Author(s):  
Gerson F Pinto ◽  
Vera L A Costa-Carvalho ◽  
Edna R Souza ◽  
Julio S Araújo Neto
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Chromatographic Method ◽  
Screening Method ◽  
Protein Characterization ◽  
Acid Water ◽  
Acetic Acid Water ◽  
Circular Paper ◽  
Excellent Reproducibility

Abstract A circular paper chromatographic method was developed for the separation of the amino acids in proteins into 7 subgroups. Butanol-acetic acid-water (4+1+1) was used as the developing solvent. Eluted ninhydrin-stained aminograms gave rise to graphic profiles or numerical indexes based on absorbance percentages. The profiles can be used to compare protein-containing samples. Twenty different samples were studied through 190 comparisons of graphic profiles and coefficients of correlation, with only 4% misleading results. The method showed excellent reproducibility for the identification or differentiation of proteins and has the advantage of being performed with low-priced apparatus and reagents.

THE PREPARATION, PAPER CHROMATOGRAPHY, AND COLOR REACTIONS OF SEVERAL 5,6-DIHYDROXYINDOLES AND 5,6-DIACETOXYINDOLES

1961 ◽  
Vol 39 (1) ◽  
pp. 231-246 ◽  
Author(s):  
R. A. Heacock ◽  
M. E. Mahon ◽  
B. D. Scott
Keyword(s):  
Acetic Acid ◽  
Paper Chromatography ◽  
Mobile Phase ◽  
Type Systems ◽  
Acid Water ◽  
Chromatographic Behavior ◽  
Acetic Acid Water ◽  
Color Reactions

The paper chromatographic behavior of several 5,6-dihydroxyindoles has been studied. The colors given by nine dihydroxyindoles with 16 chromogenic reagents are reported. The Rf values of this group of compounds on Whatman No. 1 paper with 2% acetic acid in water or n-butanol/acetic acid/water (6:1:2) as running solvents have been determined. The 5,6-dihydroxyindoles can be chromatographed satisfactorily using Zaffaroni-type systems (i.e. formamide-treated paper and non-polar running solvents). Satisfactory Rf values can also be obtained for the 5,6-diacetoxyindoles on formamide-treated paper using a suitable non-polar mobile phase. The preparations of several new 5,6-dihydroxyindoles and 5,6-diacetoxyindoles are described and improved procedures for the syntheses of some known members of these groups are reported.

Liquid Structure of Acetic Acid−Water and Trifluoroacetic Acid−Water Mixtures Studied by Large-Angle X-ray Scattering and NMR

2007 ◽  
Vol 111 (31) ◽  
pp. 9270-9280 ◽  
Author(s):  
Toshiyuki Takamuku ◽  
Yasuhiro Kyoshoin ◽  
Hiroshi Noguchi ◽  
Shoji Kusano ◽  
Toshio Yamaguchi
Keyword(s):  
Acetic Acid ◽  
Trifluoroacetic Acid ◽  
Large Angle ◽  
Liquid Structure ◽  
Acid Water ◽  
X Ray ◽  
X Ray Scattering ◽  
Acetic Acid Water ◽  
Ray Scattering

Acetic acid/water separation by pervaporation with silica filled PDMS membrane

2011 ◽  
Vol 51 (5) ◽  
pp. 819-825 ◽  
Author(s):  
Housheng Hong ◽  
Longxiang Chen ◽  
Qingwen Zhang ◽  
Zheran Zhang
Keyword(s):  
Acetic Acid ◽  
Acid Water ◽  
Pdms Membrane ◽  
Water Separation ◽  
Acetic Acid Water

Characteristics of Piperine Solubility in Multiple Solvent

2011 ◽  
Vol 236-238 ◽  
pp. 2495-2498 ◽  
Author(s):  
Xue Song Huang ◽  
Xian Zhe Lin ◽  
Mo Ting Guo ◽  
Ya Zou
Keyword(s):  
Experimental Data ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
High Performance ◽  
Authentic Sample ◽  
Allometric Model ◽  
Experiment Data ◽  
Reverse Phase ◽  
Ethanol Acetic Acid ◽  
Acetic Acid Water

The solution of piperine in multiple solvent including ethanol, acetic acid, water and HCl were investigated to extract more piperine from piper fruit. Piperine was determined by reverse phase high-performance liquid chromatography with Diamonsil column (C18,5 μm ,250 mm×4. 6 mm) at 343 nm. Experiment data were simulated by Allometric model and the formula is Z=0.9+ 4.54×10-10×x5.675+1.8029×y2.12848+2.37×10-10×x5.675×y2.12848(Z:sample solution,mol/mL,x: the percentage of ethanol’s volume, ml/100mL,y: the acetic acid in the authentic sample solution, g/100mL), the adj·R2=0.997, the comparative deviation less than 2%. These results are good in agreement with experimental data. It reveals that the model can meet the requirements of the selection and design in extracting piperine from piper fruit.

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