High-Pressure Liquid Chromatographie Determination of Resmethrin and Ratios of the cis-trans Isomers

1976 ◽  
Vol 59 (5) ◽  
pp. 1101-1103
Author(s):  
James M Zehner ◽  
Richard A Simonaitis
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Liquid Phase ◽  
High Pressure Liquid Chromatography ◽  
Internal Standard ◽  
Trans Isomers

Abstract Total resmethrin ((5-benzyl-3-furyl)methyl cis, trans-(±)-2,2-dimethyl-3-(2-methylpropenyl)cyclopropanecarboxylate) in formulations and cis-trans ratios were determined by high-pressure liquid chromatography on a column of octadecylsilane on Zipax with methanol-water liquid phase. Pentachlorobenzene was the internal standard. The cis-trans ratio can be determined directly without hydrolysis to chrysanthemumic acid as in some other methods.

A Simple Procedure for the Determination of Carbamazepine in Plasma by High Pressure-Liquid Chromatography

1979 ◽  
Vol 16 (1-6) ◽  
pp. 213-216 ◽  
Author(s):  
C. K. Johnston ◽  
G. H. Lester
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Mobile Phase ◽  
Simple Procedure ◽  
Internal Standard ◽  
Reverse Phase ◽  
Coefficients Of Variation ◽  
Initial Results

We describe a simple, rapid procedure for the estimation of carbamazepine in plasma. Protein is precipitated, and extraction is achieved by the addition of acetonitrile containing the internal standard N-acetyltryptophan ethyl ester. Separation is by reverse-phase high-pressure liquid chromatography with an acetonitrile: water mobile phase, and detection is by UV absorption at 280 nm. Total retention time is less than 7 minutes. Initial results gave within-batch and between-batch coefficients of variation of less than 2 %, and mean recovery of 97 %. The method is free from interference by other common anticonvulsant drugs.

Determination of acetaminophen concentrations in serum by high-pressure liquid chromatography.

1977 ◽  
Vol 23 (9) ◽  
pp. 1596-1598 ◽  
Author(s):  
R A Horvitz ◽  
P I Jatlow
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Diethyl Ether ◽  
High Pressure Liquid Chromatography ◽  
Internal Standard ◽  
Reversed Phase ◽  
Single Extraction ◽  
Toxic Concentrations

Abstract We describe a method for determination of serum acetaminophen concentrations in serum by reversed phase high-pressure liquid chromatography. The homolog N-propionyl-p-aminophenol was used as an internal standard. The procedure, which requires only a single extraction with diethyl ether, can be optimized to be linear over the ranges of 10 to 100 or 1 to 20 mg/liter. Within-run CV was 1.2%; between-run CV was 4.4% and 4.9% at two different concentrations. Many commonly used drugs were tested and found not to interfere. The procedure is simple and rapid enough for use on an emergency basis in cases of overdosage, and can be optimized for measurement of either therapeutic or toxic concentrations.

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