Isolation by high-pressure liquid chromatography of cis-trans isomers of β-apo-12′-carotenal and determination of their configurations by 1H NMR spectroscopy

1995 ◽  
Vol 51 (11) ◽  
pp. 1949-1958 ◽  
Author(s):  
Ying Hu ◽  
Tadashi Mizoguchi ◽  
Yoshitaka Kurimoto ◽  
Yasushi Koyama
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
High Pressure Liquid Chromatography ◽  
1H Nmr Spectroscopy ◽  
Trans Isomers

A Study of the Metabolome of Ricinus communis for Forensic Applications

2010 ◽  
Vol 63 (1) ◽  
pp. 8 ◽  
Author(s):  
Simon P. B. Ovenden ◽  
Benjamin R. Gordon ◽  
Christina K. Bagas ◽  
Bob Muir ◽  
Simone Rochfort ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
East Coast ◽  
Ricinus Communis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Nmr Data

Investigations were undertaken to ascertain the appropriateness of studying the metabolome of Ricinus communis for cultivar and provenance determination. Seeds from 14 R. communis specimens (a total of 56 seeds) collected from the east coast of Australia were analyzed by high pressure liquid chromatography with UV detection (HPLC-UV), liquid chromatography–mass spectrometry (LC-MS), and 1H NMR spectroscopy. The collected data were then analyzed using principle component analysis (PCA). For HPLC-UV analysis, six R. communis specimens were unambiguously identified by PCA as belonging to separate classes relating to specimen. LC-MS data allowed unique ions to be identified for four specimens. Conversely 10 specimens were unambiguously segregated in the PCA of the 1H NMR data. The ratio of ricinine 1 to demethylricinine analogues 2 and 3 was found to be important for specimen determination. These combined analyses suggested that a combination of HPLC-UV and 1H NMR in conjunction with PCA could allow for specimen differentiation.

High-Pressure Liquid Chromatographie Determination of Resmethrin and Ratios of the cis-trans Isomers

1976 ◽  
Vol 59 (5) ◽  
pp. 1101-1103
Author(s):  
James M Zehner ◽  
Richard A Simonaitis
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Liquid Phase ◽  
High Pressure Liquid Chromatography ◽  
Internal Standard ◽  
Trans Isomers

Abstract Total resmethrin ((5-benzyl-3-furyl)methyl cis, trans-(±)-2,2-dimethyl-3-(2-methylpropenyl)cyclopropanecarboxylate) in formulations and cis-trans ratios were determined by high-pressure liquid chromatography on a column of octadecylsilane on Zipax with methanol-water liquid phase. Pentachlorobenzene was the internal standard. The cis-trans ratio can be determined directly without hydrolysis to chrysanthemumic acid as in some other methods.

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