Multiple Internal Standard Technique for the Gas-Liquid Chromatographic Determination of Indole in Shrimp
Abstract A multiple internal standard technique has been developed for the official first action gas-liquid chromatographic (GLC) method for determining indole in shrimp. The modification was developed because interfering GLC peaks are occasionally observed when 2-methylindole is used as the internal standard. An internal standard solution containing 1-methylindole, 2- methylindole, and diphenylamine was added to extracts of shrimp before silica gel cleanup and separation by GLC. All of the compounds were quantitatively recovered and were separated on the GLC column under identical experimental conditions. Extracts of acceptable shrimp to which indole was added at levels of 3–10 μg/ 100 g and extracts of decomposed shrimp were stored at room temperature for 2 weeks. Average and maximum changes (μg indole/100 g) during storage were, respectively, for each internal standard: average 0.6, 0.4, and 1.1; maximum 1.7, 0.9, and 2.9.