Oil Flotation Extraction of Light Filth from Ground Capsicums Excluding Paprika: Collaborative Study

1978 ◽  
Vol 61 (4) ◽  
pp. 900-902
Author(s):  
Joel J Thrasher ◽  
Emma Jean Colliflower
Keyword(s):  
Collaborative Study ◽  
Fold Increase ◽  
Mineral Oil ◽  
Improved Method ◽  
Coefficients Of Variation ◽  
Filter Papers ◽  
Collaborative Tests ◽  
Wet Sieving

Abstract A collaborative study has been completed on an improved method for the isolation of light filth from ground capsicums other than paprika. The proposed method involves isopropanol pretreatment, wet-sieving, and extraction from cooled 6 0% ethanol with a mineral oil-heptane mixture. The collaborative tests by the proposed method showed an approximate 2-fold increase in recoveries of insect fragments and rodent hairs with acceptable coefficients of variation and clean filter papers. The proposed method has been adopted as official first action to replace 44.123.

Extraction of Light Filth from Tea: Collaborative Study

1981 ◽  
Vol 64 (2) ◽  
pp. 287-290
Author(s):  
Faith F Lim ◽  
◽  
J Barnett ◽  
A Bright ◽  
M P Chaput ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Collaborative Study ◽  
Tween 80 ◽  
Mineral Oil ◽  
Plant Residue ◽  
Official Method ◽  
Existing Problems ◽  
Aoac Method ◽  
Filter Papers ◽  
Wet Sieving

Abstract The present AOAC method for determining insect and rodent filth in tea is time-consuming because it produces filter papers which are heavy in plant residue and therefore requires long paper-reading times. Anew method for the analysis of light filth in tea was developed to remedy existing problems and to improve recoveries. The method consists of the following steps: sample preparation, wet sieving, dilution with 40% isopropanol, extraction with Tween 80-Na4EDTA-40% isopropanol, flotation with mineral oil-heptane, and trapping off in a Wildman trap flask. In an interlaboratory collaborative study, analysts reported combined insect fragment recoveries of 99.2% for the proposed method and 93.0% for the AOAC method; the same analysts recorded combined rodent hair recoveries of 92.2% for the proposed method and 47.6% for the official method. Average times for reading individual subsamples were 9 min for the proposed method and 27 min for the AOAC method. The proposed method has been adopted official first action.

Collaborative Study of an Enzymatic Digestion Method for the Isolation of Light Filth from Ground Beef or Hamburger

1976 ◽  
Vol 59 (1) ◽  
pp. 51-52
Author(s):  
Phillip Alioto ◽  
Mary Andreas
Keyword(s):  
Collaborative Study ◽  
Ground Beef ◽  
Enzymatic Digestion ◽  
Mineral Oil ◽  
Digestion Method ◽  
Light Mineral ◽  
Filter Papers ◽  
Wet Sieving

Abstract Collaborative results are presented for a proposed method for light filth extraction from ground beef or hamburger. The method involves enzymatic digestion, wet sieving, and extraction with light mineral oil from 40% isopropanol. Recoveries are good and filter papers are clean. This method has been adopted as official first action.

Collaborative Study of a Modified Method for the Extraction of Light Filth from Ground White Pepper, Cardamom, Celery Seed, Coriander, and Ginger

1977 ◽  
Vol 60 (1) ◽  
pp. 117-121
Author(s):  
Russell G Dent
Keyword(s):  
Collaborative Study ◽  
Mineral Oil ◽  
Improved Method ◽  
White Pepper ◽  
Modified Method ◽  
Celery Seed ◽  
Action Methods ◽  
Wet Sieving

Abstract An improved method has been developed for the extraction of light filth from 5 ground spices, ginger, white pepper, coriander, celery seed, and cardamom. The method, a modification of 44.120, utilizes a cold isopropanol defatting, followed by wet sieving and flotation of light filth from 40% isopropanol with HCl and mineral oil-heptane (85+15). Collaborative results show that the proposed method is more rapid to perform than the present official first action methods, 44.116 and 44.120, and yields better recoveries. The method has been adopted as official first action.

Extraction of Light Filth from Ground Allspice: Collaborative Study

1980 ◽  
Vol 63 (6) ◽  
pp. 1266-1268
Author(s):  
Russell G Dent ◽  
◽  
J G Eye ◽  
C C Freeman ◽  
E Hall ◽  
...  
Keyword(s):  
Hydrochloric Acid ◽  
Collaborative Study ◽  
Tween 80 ◽  
Mineral Oil ◽  
Improved Method ◽  
Aoac Method ◽  
Light Mineral ◽  
Wet Sieving

Abstract The present official AOAC method (44.120(a)(b), 13th Ed.) for extracting light filth from ground allspice uses flammable solvents and gives poor filth recoveries. An improved method has been developed which uses a single pretreatment with 40% isopropanol and hydrochloric acid, followed by wet sieving. After deaeration by boiling, the light filth is extracted from 40% isopropanol-Tween 80-tetrasodium EDTA with light mineral oil. Reports from all 6 collaborators showed that the proposed method resulted in 90 and 89% average recoveries of rodent hairs and insect fragments, respectively. The proposed method is recommended for adoption as official first action to replace AOAC (44.120(a)(b)) for allspice.

Collaborative Study of an Acid Autoclave Method for Mineral Oil Extraction of Light Filth from Spaghetti and Macaroni

1969 ◽  
Vol 52 (3) ◽  
pp. 463-465
Author(s):  
Joel J Thrasher
Keyword(s):  
Collaborative Study ◽  
Mineral Oil ◽  
Oil Extraction ◽  
New Method ◽  
Official Method ◽  
Plain Weave ◽  
Filter Papers ◽  
Wet Sieving ◽  
Better Than

Abstract A method for the extraction of light filth from alimentary pastes based on rapid autoclaving of acidified samples, wet sieving on a No. 230 plain weave sieve, and heating the sieve retainings in an acidic mineral oil mixture is described. This new method is faster and results in higher and more reproducible recoveries than method 36.025. Recoveries were equal or better than those achieved by the official method, and the filter papers were cleaner.

Collaborative Study of a Method for the Isolation of Light Filth from Breading of Frozen Food Products

1975 ◽  
Vol 58 (3) ◽  
pp. 441-442
Author(s):  
John E Kvenberg
Keyword(s):  
Collaborative Study ◽  
Food Products ◽  
Mineral Oil ◽  
Frozen Food ◽  
Collaborative Studies ◽  
Filter Papers ◽  
Wet Sieving

Abstract A method has been developed for the isolation of light filth from food breadings. The method involves a detergent boil, wet sieving, and flotation in an acid-alcohol, mineral oil flotation system in a Corning percolator. Collaborative studies resulted in clean filter papers and acceptable recoveries of added rodent hairs and insect fragments. The method has been adopted as official first action.

Determination of Internal Insect Infestation of Oats: Collaborative Study

1985 ◽  
Vol 68 (4) ◽  
pp. 699-700
Author(s):  
Richard L Trauba
Keyword(s):  
Collaborative Study ◽  
Tween 80 ◽  
Mineral Oil ◽  
Insect Infestation ◽  
Improved Method ◽  
Adult Insect ◽  
Light Mineral ◽  
Hydrolysis Of ◽  
Wet Sieving

Abstract An improved method has been developed for determining internal insect infestation of oat kennels. The method involves alcohol defatting and acid hydrolysis of the cracked oats, wet sieving to remove the acid, transfer to a 2 L Wildman trap flask, deaeration by boiling, and treatment with Tween 80-Na.tEDTA. Insects are extracted with light mineral oil. Reports from 6 collaborators showed that recoveries averaged 88.98% for adult insect heads and 97.22% for larvae. The method has been adopted official first action.

Thin Layer Chromatographic Method for the Detection of Uric Acid: Collaborative Study

1978 ◽  
Vol 61 (4) ◽  
pp. 903-905
Author(s):  
Joel J Thrasher ◽  
Annette Abadie
Keyword(s):  
Uric Acid ◽  
Thin Layer ◽  
Chromatographic Method ◽  
Phosphotungstic Acid ◽  
Collaborative Study ◽  
Lithium Carbonate ◽  
Improved Method ◽  
Color Development ◽  
Collaborative Tests ◽  
Layer Chromatography

Abstract A collaborative study has been completed on an improved method for the detection and confirmation of uric acid from bird and insect excreta. The proposed method involves the lithium carbonate solubilization of the suspect excreta material, followed by butanol-methanol-water-acetic acid thin layer chromatography, and trisodium phosphate-phosphotungstic acid color development. The collaborative tests resulted in 100% detection of uric acid standard at the 50 ng level and 75% detection at the 20–25 ng level. No false positives were reported during tests of compounds similar to uric acid. The proposed method has been adopted official first action; the present official final action method, 44.161, will be retained for screening purposes.

Extraction of Light Filth from Whole Peppermint Leaves: Collaborative Study

1985 ◽  
Vol 68 (4) ◽  
pp. 697-698
Author(s):  
Jack Boese ◽  
Marvin Nakashima ◽  
Larry E Glaze
Keyword(s):  
Collaborative Study ◽  
Tween 80 ◽  
Mineral Oil ◽  
Isopropanol Solution ◽  
Wet Sieving

Abstract Results are reported for a collaborative study of a method for the extraction of light filth from whole peppermint leaves. A 5 g sample is defatted with isopropanol in a simple reflux appartus. Rat hairs, insect fragments, and whole insects are isolated by wet sieving on a No. 230 sieve, a deaerating boil in 40% isopropanol solution, flotation with Tween 80-Na4edta (1 + 1) and mineral oil-heptane (85 + 15), and trappings in a Wildman trap flask. Average recoveries obtained by 6 collaborators for 3 spike levels of rat hairs (5,10,15) were 83.3, 87.5, and 82.2%, respectively. For whole insects (5,10,15) recoveries averaged 85.0, 80.0 and 77.2% respectively; for insect fragments (20, 30, 50) recoveries averaged 79.6, 88.3, and 84.8%, respectively. The average recoveries for the 3 levels of each analyte were not significantly different. The method has been adopted official first action.

Extraction of Light Filth from Oriental Sauces Containing Soy Sauce, Thickeners, and Spices: Collaborative Study

1993 ◽  
Vol 76 (1) ◽  
pp. 47-49
Author(s):  
Marvin J Nakashima ◽  
◽  
J A Gallman ◽  
R R Haynos ◽  
C E Highfield ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Steam Bath ◽  
Mineral Oil ◽  
Soy Sauce ◽  
Test Portion ◽  
Wet Sieving

Abstract Results are reported for a collaborative study of a method for the extraction of light filth from oriental sauces containing soy sauce, thickeners, and spices. A100 g test portion is pretreated in a 2% solution of Tergitol Anionic 4 over a steam bath, and oils are removed by wet-sieving on No. 230 sieve. Filth is isolated from 40% isopropanol by using Na4EDTA and mineral oil. Average recoveries by 9 collaborators for 3 spike levels of rat hairs (5,10, and 15) were 84, 78, and 79%, respectively; for insect fragments (5,15, and 30), recoveries were 92, 95, and 96%, respectively. The method was adopted first action by AOAC International.

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