Liquid Chromatographic Method for Determination of Piperine in Piper nigrum (Black and White Pepper)

1988 ◽  
Vol 71 (1) ◽  
pp. 53-55 ◽  
Author(s):  
Kenneth M Weaver ◽  
Michael E Neale ◽  
Ann Laneville
Keyword(s):  
Chromatographic Method ◽  
Piper Nigrum ◽  
Chromatographic System ◽  
Reverse Phase ◽  
White Pepper ◽  
Black And White ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A method was developed for the detection and quantitation of piperine in Piper nigrum. A reverse-phase liquid chromatographic system equipped with a C18 column with detection at 340 nm was used. A rapid 1 h acetone extraction followed by solvent dilution was used to avoid sample cleanup. The detection limit is 3 ng injected piperine, with 97.5-100.5% recovery of added piperine.

Determination of Thiamphenicol in Bovine Plasma by Liquid Chromatography

1988 ◽  
Vol 71 (6) ◽  
pp. 1156-1157 ◽  
Author(s):  
Lawrence Felice ◽  
El Hassane Abdennebi ◽  
Muhammed Ashraf
Keyword(s):  
Liquid Chromatography ◽  
Chromatographic Method ◽  
Reverse Phase ◽  
Reverse Phase Liquid Chromatography ◽  
Bovine Plasma ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method is described for the measurement of thiamphenicol in bovine plasma. The plasma (1 mL) is extracted with ethyl acetate. After the solvent is evaporated under a stream of nitrogen, the residue is reconstituted in methanol-water and analyzed by reverse-phase liquid chromatography with UV detection at 224 nm. The intra-day recoveries for bovine plasma spiked with 5 and 50 μg/mL of thiamphenicol were 102 and 101%, respectively, with coefficients of variation of 2.40 and 0.28%, respectively. The interday recoveries for the 5 and 50 μg/mL samples were 103 and 101%, respectively, with coefficients of variation of 3.40 and 0.94%, respectively. The sensitivity of the method allows quantitation to at least the 100 ng/mL level

Liquid Chromatographic Determination of Methyldopa and Methyldopa-Thiazide Combinations in Tablets: Collaborative Study

1984 ◽  
Vol 67 (6) ◽  
pp. 1118-1120
Author(s):  
Ting Susan ◽  
◽  
R L Brown ◽  
L A Dougherty ◽  
J B Schepman ◽  
...  
Keyword(s):  
Chromatographic Method ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Repeatability And Reproducibility ◽  
Liquid Chromatographic

Abstract A reverse phase liquid chromatographic method for the determination of methyldopa, methyldopa-hydrochlorothiazide, and methyldopachlorothiazide in tablets was collaboratively studied by 8 laboratories. Each collaborator received 20 samples that included drug substance, synthetic and commercial tablet compositions. The overall repeatability and reproducibility standard deviations for commercial tablets were 1.11 and 1.75% for methyldopa, 0.96 and 1.62% for chlorothiazide, and 1.21 and 2.15% for hydrochlorothiazide, respectively. The overall recoveries of methyldopa, chlorothiazide, and hydrochlorothiazide added to synthetic tablets were 100.78, 100.70, and 101.34%, respectively. The method has been adopted official first action.

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